Comparison of two different enzyme immunoassays in the diagnosis of fasciola hepatica infections

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dc.authorscopusid6507955227
dc.authorscopusid7005592545
dc.authorscopusid9745231700
dc.contributor.authorŞakru N.
dc.contributor.authorKorkmaz M.
dc.contributor.authorKuman H.A.
dc.date.accessioned2024-06-12T10:28:07Z
dc.date.available2024-06-12T10:28:07Z
dc.date.issued2004
dc.description.abstractIn this study, the serum samples of 37 patients who were diagnosed as fascioliasis (=fasciolosis) by parasitologic, pathologic and/or radiographic methods, 51 patients who were previously infected with parasites other than Fasciola hepatica, and 31 healthy subjects, have been investigated for the presence of F.hepatica specific lgG antibodies with two different enzyme immunoassay (ELISA) methods. For this purpose, in-house DOT-ELISA and excretory-secretory (ES)-ELISA techniques have been performed by using F.hepatica ES antigens. All of the 37 fasciolosis patients found to be seropositive with ES-ELISA, while 36 were found positive with DOT-ELISA method. ES-ELISA method yielded 3 (5.9%) positive results for the patients with other parasitosis (2 toxocariasis, 1 hydatic cyst), and DOT-ELISA method gave positive results for 2 (3.9%) toxocariasis patients. In 31 healthy subjects, the seropositivity rates were detected as 3.2% and 16.1% for ES-ELISA and DOT-ELISA methods, respectively. As a result, the sensitivity and specificity of DOT-ELISA method were found as 97.3% and 93.3%, respectively, while these rates were 100% and 97.0% for ES-ELISA, respectively. The highest positive predictive value was detected for ES-ELISA (90.2%), while both of the methods showed high negative predictive values (100% for ES-ELISA, 99.1% for DOT-ELISA). In conclusion, ELISA methods would be useful especially for the laboratory diagnosis of fasciolosis, in the clinically suspected patients where the etiologic agent could not be identified.en_US
dc.identifier.endpage135en_US
dc.identifier.issn0374-9096
dc.identifier.issue1.Şuben_US
dc.identifier.pmid15293912en_US
dc.identifier.scopus2-s2.0-28744434413en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage129en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/17072
dc.identifier.volume38en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectElisa; Fasciola Hepatica; Fascioliasis; Fasciolosisen_US
dc.subjectHelminth Antibody; Immunoglobulin G; Animal; Article; Blood; Comparative Study; Enzyme Linked Immunosorbent Assay; Fasciola Hepatica; Fascioliasis; Human; Immunology; Methodology; Animals; Antibodies, Helminth; Enzyme-Linked Immunosorbent Assay; Fasciola Hepatica; Fascioliasis; Humans; Immunoglobulin Gen_US
dc.titleComparison of two different enzyme immunoassays in the diagnosis of fasciola hepatica infectionsen_US
dc.title.alternativeFasciola hepatica enfeksiyonunun laboratuvar tanisinda iki farkli enzim immün yönteminin karşilaştirilmasien_US
dc.typeArticleen_US

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