Evaluation of miRNAs Related with Nuclear Factor Kappa B Pathway in Lipopolysaccharide Induced Acute Respiratory Distress Syndrome

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dc.authoridBayrak, Gülsen/0000-0002-1397-7203
dc.authoridAzizoğlu, Mustafa/0000-0002-8266-5203
dc.authoridAzizoğlu, Mustafa/0000-0002-8266-5203
dc.authoridCoskun Yilmaz, Banu/0000-0002-7723-9146
dc.authorwosidAyaz, Lokman/K-6716-2013
dc.authorwosidBayrak, Gülsen/HJP-9011-2023
dc.authorwosidAzizoğlu, Mustafa/G-9035-2016
dc.authorwosidMutlu, Gülşen/KCJ-8141-2024
dc.authorwosidAzizoğlu, Mustafa/ABD-1206-2021
dc.authorwosidCoşkun Yılmaz, Banu/KAQ-6923-2024
dc.contributor.authorAzizoglu, Mustafa
dc.contributor.authorAyaz, Lokman
dc.contributor.authorBayrak, Gulsen
dc.contributor.authorYilmaz, Banu Coskun
dc.contributor.authorBirbicer, Handan
dc.contributor.authorDoruk, Nurcan
dc.date.accessioned2024-06-12T10:50:37Z
dc.date.available2024-06-12T10:50:37Z
dc.date.issued2020
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThis study aimed to determine the expression of nuclear factor kappa B (NF-kappa B) pathway related miRNAs in experimental acute respiratory distress syndrome (ARDS) induced by lipopolysaccharide (LPS) in rats, and to elucidate the underlying molecular mechanism. Twenty four sprague dawley rats were randomly divided into two groups; LPS (n = 12) and control (n = 12). Experimental ARDS was induced by intraperitoneal injection of E. coli LPS in LPS group. Intraperitoneal saline was administered in control group. Serum and lung samples were collected from both groups. Inununohistochemistry staining was performed for interleukin 1 beta (IL-beta), tumor necrosis factor alpha (TNF-alpha), CD 68, and caspase-3 in lung samples. Intensity of staining was scored as strong, moderate, weak, and no for evaluation of IL-1 beta and TNF-alpha. In addition, caspase-3 and CD68-positive stained cells were counted in sections. Expressions of 9 miRNAs were determined by quantitative real-time PCR in serum samples. IL-1 beta and TNF-alpha staining scores were significantly higher in the LPS group in comparison with the control group (P = 0.04 and P = 0.02, respectively). In addition, caspase-3 and CD68-positive stained cells were significantly higher in the LPS group (P = 0.02). Expressions of seven miRNAs were significantly changed in the LPS group in comparison with the control group. While six miRNAs (miR-7a-5p, miR-7b, miR9a-5p, miR-21-5p, miR-29a-3p, and miR-138-5p) were up regulated, only miR-124-3p was down regulated. This study suggests that these miRNAs may have a role in the pathogenesis of ARDS related to NF-kappa B. However, this relationship needs to be examined in new studies by evaluation of pathways and target genes.en_US
dc.description.sponsorshipMersin University Scientific Researsch Project Unit [2016-2-AP3-1914]en_US
dc.description.sponsorshipThis study was supported by Mersin University Scientific Researsch Project Unit (Grant: 2016-2-AP3-1914).en_US
dc.identifier.doi10.22088/IJMCM.BUMS.9.2.130
dc.identifier.endpage139en_US
dc.identifier.issn2251-9637
dc.identifier.issn2251-9645
dc.identifier.issue2en_US
dc.identifier.pmid32934950en_US
dc.identifier.startpage130en_US
dc.identifier.urihttps://doi.org/10.22088/IJMCM.BUMS.9.2.130
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18070
dc.identifier.volume9en_US
dc.identifier.wosWOS:000605926700003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCellular & Molecular Biology Research Centeren_US
dc.relation.ispartofInternational Journal Of Molecular And Cellular Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAcute Respiratory Distress Syndromeen_US
dc.subjectARDSen_US
dc.subjectMicrornaen_US
dc.subjectNuclear Factor Kappa Ben_US
dc.subjectAcute Lung Injuryen_US
dc.subjectMicrornasen_US
dc.subjectExpressionen_US
dc.subjectNeutrophilsen_US
dc.subjectModelen_US
dc.titleEvaluation of miRNAs Related with Nuclear Factor Kappa B Pathway in Lipopolysaccharide Induced Acute Respiratory Distress Syndromeen_US
dc.typeArticleen_US

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