Flow Cytometric Evaluation of Pregnancy-Induced Anti-Donor Immunization

Basit Öğe Kaydı
dc.authoridgürtekin, başak/0000-0001-8806-2270
dc.authoridIsitmangil, Gulbu/0000-0003-4243-8003
dc.authorwosidIşıtmangil, Gülbu/JVN-8197-2024
dc.authorwosidCiftci, Hayriye Senturk/AAD-3814-2020
dc.authorwosidgürtekin, başak/AAA-6231-2020
dc.contributor.authorAyna, T. K.
dc.contributor.authorCiftci, H. S.
dc.contributor.authorTozkir, H.
dc.contributor.authorSagiroglu, T.
dc.contributor.authorIsitmangil, G.
dc.contributor.authorGurtekin, B.
dc.contributor.authorGurtekin, M.
dc.date.accessioned2024-06-12T11:19:58Z
dc.date.available2024-06-12T11:19:58Z
dc.date.issued2012
dc.departmentTrakya Üniversitesien_US
dc.description8th Congress of the Turkish-Transplantation-Centers-Coordination-Association (TTCCA) -- OCT 12-16, 2011 -- Antalya, TURKEYen_US
dc.description.abstractBackground. Living donor kidneys from spouses and children (from offspring to parents) are currently considered to be important organ sources. However, pregnancy-induced alloimmunization may provoke acute rejection episodes after kidney transplantation, being flow cytometry cross-match (FCXM) we studied donor-specific antibodies (DSAs) in the sera of recipients planned for living kidney transplantation from their spouse or children. When the FCXM was positive, we confirmed the existence of anti-human leukocyte antigen (H LA) antibodies using flow cytometry panel-reactive antibody (flow-PRA). Patients and Methods. Between March 2005 and November 2010, we tested 85 pretransplantation sera from renal transplant recipients for DSAs. The recipients included 37 wives (group I) and 48 husbands (group II). FCXM-positive sera were tested using a flow-PRA screening method using HLA class I and class II antigen-coated beads. The mean recipient age was 48.1 +/- 9.8 (range, 28-69) years and the mean donor age was 45.1 +/- 11.1 (range, 23-69 years). Results. Among group I were 18 (48.6%) FCXM-positive cross-matches; for group II, 5 (10.4%) cases (P = .001). Sensitized patients were 37.9% FCXM-positive, whereas nonsensitized patients were 3.7% positive (P = .001). FCXM-positive patients were re-evaluated for anti-HLA antibodies using flow-PRA. Seventeen of 18 group I tests (94.4%) were FCXM-positive, whereas 3 of 5 (60%) were positive among group II. Conclusions. We concluded that flow cytometry-based cross-match and PRA techniques can be used to detect anti-HLA antibodies using spousal or children donors for kidney transplantation.en_US
dc.description.sponsorshipTurkish Transplantat Ctr Coordinat Assoc (TTCCA)en_US
dc.identifier.doi10.1016/j.transproceed.2012.04.027
dc.identifier.endpage1659en_US
dc.identifier.issn0041-1345
dc.identifier.issue6en_US
dc.identifier.pmid22841238en_US
dc.identifier.scopus2-s2.0-84864402257en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1656en_US
dc.identifier.urihttps://doi.org/10.1016/j.transproceed.2012.04.027
dc.identifier.urihttps://hdl.handle.net/20.500.14551/25391
dc.identifier.volume44en_US
dc.identifier.wosWOS:000307433400053en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTransplantation Proceedingsen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSingle-Center Experienceen_US
dc.subjectOffspring-To-Motheren_US
dc.subjectRenal-Transplantationen_US
dc.subjectKidney-Transplantationen_US
dc.subjectSpousalen_US
dc.titleFlow Cytometric Evaluation of Pregnancy-Induced Anti-Donor Immunizationen_US
dc.typeConference Objecten_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu