Lung Oxidative Stress, DNA Damage, Apoptosis, and Fibrosis in Adenine-Induced Chronic Kidney Disease in Mice

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dc.authoridKARACA, Turan/0000-0002-2500-7781
dc.authoridYUVARAJU, PRIYADHARSHINI/0000-0003-2383-457X
dc.authoridPATHAN, JAVED YASIN/0000-0002-5867-2833
dc.authoridNemmar, Abderrahim/0000-0002-0699-1015
dc.authorwosidPATHAN, JAVED YASIN/GOE-4396-2022
dc.authorwosidKARACA, Turan/ABD-6669-2020
dc.contributor.authorNemmar, Abderrahim
dc.contributor.authorKaraca, Turan
dc.contributor.authorBeegam, Sumaya
dc.contributor.authorYuvaraju, Priya
dc.contributor.authorYasin, Javed
dc.contributor.authorAli, Badreldin H.
dc.date.accessioned2024-06-12T10:50:41Z
dc.date.available2024-06-12T10:50:41Z
dc.date.issued2017
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIt is well-established that there is a crosstalk between the lung and the kidney, and several studies have reported association between chronic kidney disease (CKD) and pulmonary pathophysiological changes. Experimentally, CKD can be caused in mice by dietary intake of adenine. Nevertheless, the consequence of such intervention on the lung received only scant attention. Here, we assessed the pulmonary effects of adenine (0.2% w/w in feed for 4 weeks)-induced CKD in mice by assessing various physiological histological and biochemical endpoints. Adenine treatment induced a significant increase in urine output, urea and creatinine concentrations, and it decreased the body weight and creatinine clearance. It also increased proteinuria and the urinary levels of kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin. Compared with control group, the histopathological evaluation of lungs from adenine-treated mice showed polymorphonuclear leukocytes infiltration in alveolar and bronchial walls, injury, and fibrosis. Moreover, adenine caused a significant increase in lung lipid peroxidation and reactive oxygen species and decreased the antioxidant catalase. Adenine also induced DNA damage assessed by COMET assay. Similarly, adenine caused apoptosis in the lung characterized by a significant increase of cleaved caspase-3. Moreover, adenine induced a significant increase in the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) in the lung. We conclude that administration of adenine in mice induced CKD is accompanied by lung oxidative stress, DNA damage, apoptosis, and Nrf2 expression and fibrosis.en_US
dc.description.sponsorshipUnited Arab Emirates University-Sultan Qaboos university joint grant; CMHS; UPAR; Center-based interdisciplinary grantsen_US
dc.description.sponsorshipThis work was supported by a United Arab Emirates University-Sultan Qaboos university joint grant, CMHS, UPAR, and Center-based interdisciplinary grants. We would like to thank Dr. Abderrahim Oulhaj from the Institute of Public Health, CMHS, UAEU for his statistical advices.en_US
dc.identifier.doi10.3389/fphys.2017.00896
dc.identifier.issn1664-042X
dc.identifier.pmid29218013en_US
dc.identifier.scopus2-s2.0-85034978768en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3389/fphys.2017.00896
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18088
dc.identifier.volume8en_US
dc.identifier.wosWOS:000415992200001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherFrontiers Media Saen_US
dc.relation.ispartofFrontiers In Physiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAdenineen_US
dc.subjectChronic Renal Diseaseen_US
dc.subjectLungen_US
dc.subjectOxidative Stressen_US
dc.subjectApoptosisen_US
dc.subjectDNA Damageen_US
dc.subjectNrf2en_US
dc.subjectChronic-Renal-Failureen_US
dc.subjectDiesel Exhaust Particlesen_US
dc.subjectAirway-Resistanceen_US
dc.subjectComet Assayen_US
dc.subjectMouse Modelen_US
dc.subjectGum Acaciaen_US
dc.subjectRatsen_US
dc.subjectPulmonaryen_US
dc.subjectInflammationen_US
dc.subjectMechanismsen_US
dc.titleLung Oxidative Stress, DNA Damage, Apoptosis, and Fibrosis in Adenine-Induced Chronic Kidney Disease in Miceen_US
dc.typeArticleen_US

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