Functional and Biochemical Properties of Proteins from Safflower Seed

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dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.contributor.authorSagiroglu, Ayten
dc.contributor.authorOzcan, Hakk M.
dc.contributor.authorSatana, Aziz
dc.date.accessioned2024-06-12T10:54:36Z
dc.date.available2024-06-12T10:54:36Z
dc.date.issued2009
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThere are relatively few studies on the properties of proteins that comprise a major part of the safflower seed. The biochemical and functional properties of these proteins have not been fully discovered. In this study, safflower seed proteins were obtained by isoelectric precipitation in two fractions. One of the fractions (Fraction-1) was obtained at pH 10 and the other fraction was obtained, as the protein supernatant separate from the pH precipitate, by ultra filtration (Fraction-2). Functional and biochemical properties of both of fractions were investigated. Gel permeation chromatography (GPC) was applied to both fractions. GPC shows that high-molecular weight constituents are present only in fraction 1, whereas fraction 2 consists of proteins with lower molecular weights in comparison with protein standards. Sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) electrophoresis in the presence of mercaptoethanol and SDS with protein weight markers was applied to both of the fractions. The proteins in both of the fractions were separated and stained with Commassie Brillant blue R-250 dye on the PAGE gel. The molecular weight (Mw) of each protein band was determined graphically by plotting Log Mw and relative mobilities (Rf) using GS-300 scanning densitometry and a suitable computer program.en_US
dc.identifier.doi10.1080/10826060902800833
dc.identifier.endpage169en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue2en_US
dc.identifier.pmid19291578en_US
dc.identifier.scopus2-s2.0-65749118644en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage159en_US
dc.identifier.urihttps://doi.org/10.1080/10826060902800833
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19095
dc.identifier.volume39en_US
dc.identifier.wosWOS:000264224600006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiochemical And Functional Propertiesen_US
dc.subjectSafflower Seed (Carthamus Tintorius Len_US
dc.subject)en_US
dc.subjectSeed Proteinen_US
dc.titleFunctional and Biochemical Properties of Proteins from Safflower Seeden_US
dc.typeArticleen_US

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