Production and properties of ?-amylase from Penicillium chrysogenum and its application in starch hydrolysis

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dc.authoridErtan, Figen/0000-0002-7882-3993
dc.contributor.authorBalkan, B
dc.contributor.authorErtan, F
dc.date.accessioned2024-06-12T10:51:19Z
dc.date.available2024-06-12T10:51:19Z
dc.date.issued2005
dc.departmentTrakya Üniversitesien_US
dc.description.abstractFungi were screened for their ability to produce a-amylase by a plate culture method. Penicillium chrysogenum showed high enzymatic activity. alpha-Amylase production by P. chrysogenum cultivated in liquid media containing maltose (2%) reached its maximum at 6-8 days, at 30 degrees C, with a level of 155 U ml(-1). Some general properties of the enzyme were investigated. The optimum reaction pH and temperature were 5.0 and 30-40 degrees C, respectively. The enzyme was stable at a pH range from 5.0-6.0 and at 30 degrees C for 20 min and the enzyme's 92.1% activity's was retained at 40 degrees C for 20 min without substrate. Hydrolysis products of the enzyme were maltose, unidefined oligosaccharides, and a trace amount of glucose. alpha-Amylase of P. chrysogenum hydrolysed starches from different sources. The best hydrolysis was determined (98.69%) in soluble starch for 15 minute at 30 degrees C.en_US
dc.identifier.doi10.1081/PB-200054740
dc.identifier.endpage178en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue2en_US
dc.identifier.pmid15881598en_US
dc.identifier.scopus2-s2.0-17844361929en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage169en_US
dc.identifier.urihttps://doi.org/10.1081/PB-200054740
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18317
dc.identifier.volume35en_US
dc.identifier.wosWOS:000228669200008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectProductionen_US
dc.subjectPropertiesen_US
dc.subjectStarchen_US
dc.subjectPenicillium Chrysogenumen_US
dc.subjectAmylolytic Enzymesen_US
dc.subjectPurificationen_US
dc.subjectStrainen_US
dc.subjectFungien_US
dc.titleProduction and properties of ?-amylase from Penicillium chrysogenum and its application in starch hydrolysisen_US
dc.typeArticleen_US

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