Optimization and immobilization of urease enzyme isolated from Proteus mirabilis on alginate beads

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dc.contributor.authorTetiker, A. T.
dc.contributor.authorErtan, F.
dc.date.accessioned2024-06-12T10:51:10Z
dc.date.available2024-06-12T10:51:10Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractUrease enzyme was isolated from Proteus mirabilis and immobilized in alginate beads. Various parameters such as optimum pH, optimum temperature, pH stability, thermal stability, reusability, storage stability and substrate concentration were investigated and the findings were compared with free urease enzyme. immobilization yield was calculated as 85 %. Optimum temperature was found to be 40 degrees C for free and immobilized urease. Thermal stability of the immobilized urease enzyme was significantly better than the free enzyme. Optimum pH for free and immobilized urease was be 7.0. Immobilized and free urease enzymes protected their stability at pH 7.0 and 8.0 in a similar way. Immobilized enzyme maintained 55% of their initial activity after 12 repeated use of enzyme. It was found that storage stability of immobilized enzyme was better than that of free enzyme. K-m and V-max values from the Lineweaver-Burk plots were calculated.en_US
dc.description.sponsorshipTrakya University Research Foundation [TUBAP-2013-147]en_US
dc.description.sponsorshipThis research was supported by Trakya University Research Foundation (Project Number: TUBAP-2013-147).en_US
dc.identifier.doi10.22438/jeb/40/5/PRN-f18
dc.identifier.endpage1128en_US
dc.identifier.issn0254-8704
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85076379119en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1123en_US
dc.identifier.urihttps://doi.org/10.22438/jeb/40/5/PRN-f18
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18269
dc.identifier.volume40en_US
dc.identifier.wosWOS:000495358000020en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.ispartofJournal Of Environmental Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlginateen_US
dc.subjectImmobilizationen_US
dc.subjectProteus Mirabilisen_US
dc.subjectUreaseen_US
dc.subjectAlpha-Amylaseen_US
dc.titleOptimization and immobilization of urease enzyme isolated from Proteus mirabilis on alginate beadsen_US
dc.typeArticleen_US

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