Investigation of Polymerase Chain Reaction Method in Patients with Suspected Chronic Cutaneous Leishmania of Negative Microscopy

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dc.authorwosidUrhan-Küçük, Meral/ADN-8729-2022
dc.contributor.authorCulha, Gulnaz
dc.contributor.authorKaya, Tugba
dc.contributor.authorGulbol Duran, Gulay
dc.contributor.authorUrhan Kucuk, Meral
dc.contributor.authorCigdem Dogramaci, Asena
dc.contributor.authorTiyekli Celik, Dilek
dc.date.accessioned2024-06-12T10:56:31Z
dc.date.available2024-06-12T10:56:31Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractLeishmaniasis is a parasitic disease that is transmitted to humans by the bites of infected female phlebotomine sandflies. In the diagnosis of cutaneous leishmaniasis (CL), in the smear samples, the demonstration of the parasite by microscope remains a gold standard method. However, it becomes difficult to diagnose the parasite since the number of amastigotes in chronic cases with a lesion of one year or longer is very low. Due to many factor such as patients primarily do not to take any notice these lesions in their bodies, do not apply to health institutions or late applied, receive wrong treatment; the diagnosis and treatment are delayed. In addition, it is been worse prognosis by add secondary infection to lesions and wounds become chronic. For this reason, molecular methods are used in addition to microscopic examination in chronic suspected CL cases. It was aimed to reveal of the molecular diagnostic value in chronic suspected CL cases by polymerase chain reaction (PCR) in the smear belonging to Turkish patients that reported to be evaluated clinically because it can not be seen Leishmania amastigotes in microscopic examination. Smear of 50 Turkish patients who were clinically reported of the evaluation of chronic CL were selected. These samples were smears belonging to suspected CL patients that applied Hatay Mustafa Kemal University, Faculty of Medicine, Parasitology laboratory from different polyclinics and were decided to be evaluated clinically as a result of microscopic examination because they came from endemic regions (such as Hassa, Altinozu, Yayladagi). DNA was isolated from selected samples and PCR was performed using 13A, 13B primers targeting the kinetoplastid DNA (kDNA) region. The samples found positive by PCR were typed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using LITSR and L5.8S primers targeting internal transcribed spacer (ITS-1) region. Of the 50 smear samples, 17 (34%) were determined positive with 13A, 13B primers targeting the kinetoplastid DNA (kDNA) region. Positive samples were also found to be positive with LITSR and L5.8S primers targeting ITS-1 region. The PCR products obtained from PCR with ITS-1 gene region were digested with the restriction endonucleases BsuRl (Haelll). As a result of PCR-RFLP analysis, it was determined that 11 of Leishmania tropica, one of Leishmania major and five of Leishmania infantum/donovani out of 17 samples. Chronic CL can be confused with skin diseases such as sarcoidosis, tuberculosis, malignant tumors. In particular, chronic CL cases can be escaped the attention for many reasons such as failure to diagnose correctly, insufficient microscope experience, fail to see due to low number of parasites. For this reason, it was concluded that PCR, which is a molecular method, should be used in chronic suspected CL samples which are negative for the parasite by microscopic examination.en_US
dc.identifier.doi10.5578/mb.68692
dc.identifier.endpage418en_US
dc.identifier.issn0374-9096
dc.identifier.issue4en_US
dc.identifier.pmid31709938en_US
dc.identifier.scopus2-s2.0-85074742386en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage408en_US
dc.identifier.trdizinid377141en_US
dc.identifier.urihttps://doi.org/10.5578/mb.68692
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/377141
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19817
dc.identifier.volume53en_US
dc.identifier.wosWOS:000490256400006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChronic Suspected Leishmaniasisen_US
dc.subjectDiagnosisen_US
dc.subjectPolymerase Chain Reactionen_US
dc.subjectRestriction Fragment Length Polymorphismen_US
dc.subjectEndemic Regionen_US
dc.subjectPcr-Rflpen_US
dc.subjectDiagnosisen_US
dc.subjectIdentificationen_US
dc.subjectSamplesen_US
dc.subjectParasitesen_US
dc.subjectProvinceen_US
dc.titleInvestigation of Polymerase Chain Reaction Method in Patients with Suspected Chronic Cutaneous Leishmania of Negative Microscopyen_US
dc.typeArticleen_US

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