Behçet hastalığında Apoptozis
Yükleniyor...
Dosyalar
Tarih
2006
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Trakya Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Apoptozis programlanmış hücre ölümü olup normal immün sistemin düzeninde ve bir çok hastalığın patogenezinde önemli işlevleri vardır. Bu çalışmada Behçet Hastalarının periferik lenfositlerinde proapoptotik bir molekül olan CD95 ve antiapoptotik bir molekül olan Bcl-2 ekspresyonu araştırılarak apoptotik mekanizmaların Behçet Hastalığındaki rolü araştırılmıştır. Uluslararası Behçet Çalışma Grubu Kriterlerine uygun olarak tanısı konmuş 21 Behçet Hastası ve 32 sağlıklı kontrol çalışmaya alındı. 21 olgunun 6'sında aktif ve inaktif dönemlerinde kanlar alındığından toplam 29 örnek üzerinde çalışma yapılmıştır Hastaların detaylı klinik muayeneleri yapılarak mevcut klinik bulgular kaydedildi. Oral aft, genital ülser, eritema nodozum, artrit, göz, tromboz, nörolojik tutulum ya da gastrointestinal tutulumdan bir ya da bir kaçının bir arada olması durumunda hasta aktif kabul edildi. Hasta ve kontrol grubundan EDTA'lı tüplere kan alınarak tam kan sayımı ve çok renkli akım sitometrisi ile periferik kan lenfositler üzerindeki CD4, CD8 ve CD 95 pozitifliği oranı ve bu markerlerin ekspresyon şiddetleri ayrı ayrı tespit edildi. Ayrıca intrasitoplazmik olarak periferik kan lenfositlerinin Bcl-2 pozitiflik oranı ve ekspresyon şiddeti belirlendi. Behçet Hastaları ile sağlıklı kontrollerin ortalama CD4+, CD8+, CD95+, CD4+CD95+ ve CD8+CD95+ hücre oranları ve markerlerin ekspresyon şiddetleri bakımından aralarında fark saptanmadı (p>0.05). Behçet Hastalarının Bcl-2+ lenfosit oranı sağlıklı kontrollere göre 1 anlamlı olarak daha yüksek saptandı ( p<0.05). Ancak Bcl-2 ekspresyon şiddeti bakımından aralarında anlamlı fark saptanmadı (p>0.05). Bu veriler Behçet Hastalığında proapoptotik moleküllerin yetersiz eksprese edildiğini ancak antiapoptotik moleküllerin ekspresyonunda artış nedeni ile proapoptotik ve antiapoptotik mekanizmalar arasında dengesizlik sebebi ile herhangi bir nedenle başlamış olan inflamasyonun devamının sağlandığını düşündürmektedir.
Apoptosis is meaning of programmed cell death that has many role on normal immune system and pathogenesis of several disease. Here in we studied expression of Fas which is a proapoptotic molecule and Bcl-2 an antiapoptotic molecule on peripheral lymphocytes of patients with Behçet?s Disease and discuss it?s role on pathogenesis of Behçet?s Disease. Twenty-one patients with Behcet's Disease in according to Internaional Study Group's cirteria and 32 healthy controls were included to the study. In patient group, the study performed totally on 29 blood specimen, because of blood specimens were taken during active and inactive periods of 6 patients. After the detailed physical examination of the contributors, clinical characteristics of the patients were recorded. Patients were accepted as active in the presencese of any of the oral aphtous ulcer, genital ulcer, erythema nodosum, ostiofolliculitis, active eye disease, active arthritis, thrombophlebitis, arterial aneurisyms and neurological or gastrointestial involvement. Blood were withdrawn into EDTA contained tubes and whole blood cell analysis and expression ratio and expression indensity of the CD4, CD8 CD95 molecules on the peripheral blood lymphocytes by colored flowcytometric analysis. Intracytoplasmic Bcl-2 expression rate and expression indensity were also determined by colored flowcytometric analysis. 1 Mean ratio of the CD4+, CD8+, CD95+, CD4+CD95+ and CD8+CD95+ peripheral T lymphocytes and mean floerosein indencity of such markers of the patients and healthy controls were not significant (p>0.05). But Bcl-2 expression rate of the patients with Behcet's disease was found significantly higher than healthy controls(p:<0.05). We concluded that disturbed balance between proapoptotic and antiapoptotic mechanisms may play role in the pathogenesis of the Behcet's disease.
Apoptosis is meaning of programmed cell death that has many role on normal immune system and pathogenesis of several disease. Here in we studied expression of Fas which is a proapoptotic molecule and Bcl-2 an antiapoptotic molecule on peripheral lymphocytes of patients with Behçet?s Disease and discuss it?s role on pathogenesis of Behçet?s Disease. Twenty-one patients with Behcet's Disease in according to Internaional Study Group's cirteria and 32 healthy controls were included to the study. In patient group, the study performed totally on 29 blood specimen, because of blood specimens were taken during active and inactive periods of 6 patients. After the detailed physical examination of the contributors, clinical characteristics of the patients were recorded. Patients were accepted as active in the presencese of any of the oral aphtous ulcer, genital ulcer, erythema nodosum, ostiofolliculitis, active eye disease, active arthritis, thrombophlebitis, arterial aneurisyms and neurological or gastrointestial involvement. Blood were withdrawn into EDTA contained tubes and whole blood cell analysis and expression ratio and expression indensity of the CD4, CD8 CD95 molecules on the peripheral blood lymphocytes by colored flowcytometric analysis. Intracytoplasmic Bcl-2 expression rate and expression indensity were also determined by colored flowcytometric analysis. 1 Mean ratio of the CD4+, CD8+, CD95+, CD4+CD95+ and CD8+CD95+ peripheral T lymphocytes and mean floerosein indencity of such markers of the patients and healthy controls were not significant (p>0.05). But Bcl-2 expression rate of the patients with Behcet's disease was found significantly higher than healthy controls(p:<0.05). We concluded that disturbed balance between proapoptotic and antiapoptotic mechanisms may play role in the pathogenesis of the Behcet's disease.
Açıklama
Yüksek Lisans Tezi
Anahtar Kelimeler
Behçet Hastalığı, Apoptozis, Fas Antijeni, Bcl-2 Geni, Behcet's Disease, Apoptosis, Fas Antigen, Bcl-2 Gene
