Quercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activation

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dc.contributor.authorTopcu-Tarladacalisir, Yeter
dc.contributor.authorSapmaz-Metin, Melike
dc.contributor.authorMercan, Zeynep
dc.contributor.authorErcetin, Deniz
dc.date.accessioned2024-06-12T11:19:28Z
dc.date.available2024-06-12T11:19:28Z
dc.date.issued2024
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground: The inflammatory bowel diseases (IBD) are significantly influenced by apoptosis and endoplasmic reticulum (ER) stress. Aims: To investigate the effects of quercetin on ER stress-mediated apoptosis in a trinitrobenzene sulfonic acid (TNBS) induced experimental IBD model. Study Design: In vivo animal experimental study.Methods: To demonstrate the effect of quercetin in an experimental colitis model, Control, TNBS, and TNBS+quercetin groups were created with 24 Wistar Albino rats. Colitis was induced by intrarectal administration of 25 mg TNBS. In the TNBS+quercetin group, intragastrically 100 mg/kg quercetin was given for 7 days, immediately after colitis induction. In the TNBS-induced experimental IBD model, we evaluated the effects of quercetin on colonic epithelial cell apoptosis, oxidative stress, ER stress, the mitogen-activated protein kinase c-Jun N-terminal kinase, and the nuclear factor kappa B immunoreactivities, the levels of myeloperoxidase and tumor necrosis factor-alpha, the disease activity index with colonic histopathologic changes.Results: TNBS administration induced an elevated level of disease activity and oxidative stress indices, inflammation markers, and an increase in the immunoreactivities of nuclear factor kappa B and the mitogen-activated protein kinase c-Jun N-terminal kinase in the colon of the colitis group. Glucose regulatory protein 78, caspase-12 immunoreactivities, and epithelial cell apoptosis also were shown in the colon. However, quercetin improved TNBS-induced histopathological alterations, apoptosis, inflammation, oxidative stress, and ER stress.Conclusion: This study suggests that quercetin has a regulatory effect on ER stress-mediated apoptosis, and thus may be beneficial in treating IBD.en_US
dc.description.sponsorshipTrakya University Scientific Research Committee [2018/123]en_US
dc.description.sponsorshipFunding: The study was supported by a grant provided by the Trakya University Scientific Research Committee (project number: 2018/123) .en_US
dc.identifier.doi10.4274/balkanmedj.galenos.2023.2023-10-9
dc.identifier.endpage37en_US
dc.identifier.issn2146-3123
dc.identifier.issn2146-3131
dc.identifier.issue1en_US
dc.identifier.pmid38173174en_US
dc.identifier.scopus2-s2.0-85181776014en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage30en_US
dc.identifier.urihttps://doi.org/10.4274/balkanmedj.galenos.2023.2023-10-9
dc.identifier.urihttps://hdl.handle.net/20.500.14551/25210
dc.identifier.volume41en_US
dc.identifier.wosWOS:001146244200004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofBalkan Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNf-Kappa-Ben_US
dc.subjectIntestinal Epithelial-Cellsen_US
dc.subjectInflammatory-Bowel-Diseaseen_US
dc.subjectOxidative Stressen_US
dc.subjectEr Stressen_US
dc.subjectNitric-Oxideen_US
dc.subjectMacrophagesen_US
dc.subjectExpressionen_US
dc.subjectCaspase-12en_US
dc.subjectPathwayen_US
dc.titleQuercetin Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis by Inhibiting the Glucose Regulatory Protein 78 Activationen_US
dc.typeArticleen_US

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