Role of L-carnitine in the prevention of seminiferous tubules damage induced by gamma radiation: a light and electron microscopic study

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dc.authorwosidUZAL, Mustafa Cem/AAH-2683-2019
dc.contributor.authorTopcu-Tarladacalisir, Yeter
dc.contributor.authorKanter, Mehmet
dc.contributor.authorUzal, Mustafa Cem
dc.date.accessioned2024-06-12T11:02:26Z
dc.date.available2024-06-12T11:02:26Z
dc.date.issued2009
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe present study, we hypothesized that l-carnitine can minimize germ-cell depletion and morphological features of late cell damage in the rat testis following gamma (gamma)-irradiation. Wistar albino male rats were divided into three groups. Control group received physiological saline 0.2 ml intraperitoneally (i.p.), as placebo. Radiation group received scrotal gamma-irradiation of 10 Gy as a single dose plus physiological saline. Radiation + l-carnitine group received scrotal gamma-irradiation plus 200 mg/kg i.p. l-carnitine. l-carnitine starting 1 day before irradiation and 21 days (three times per week) after irradiation. Testis samples of the all groups were taken at day 21, 44 and 70 post-irradiation. All samples were processed at the light and electron microscopic levels. Morphologically, examination of gamma-irradiated testis revealed presence of marked disorganization and depletion of germ cells, arrest of spermatogenesis, formation of multinucleated giant cells, and vacuolization in the germinal epithelium. The type and extent of these changes varied at different post-treatment intervals. The damage was evident at the 21st day and reached maximum level by the 44th day. By day 44 post-irradiation, the changes were most advanced, and were associated with atrophied seminiferous tubules without germ cells, the increase in the number and size of vacuolizations in germinal epithelium, and the absent multinucleated giant cells due to spermatids had completely disappeared. The increase in nucleus invaginations, the dilatation of smooth endoplasmic reticulum cysternas and the increase in the number and size of lipid droplets in the Sertoli cells were determined at the electron microscopic level. In conclusion, l-carnitine supplementation during the radiotherapy would be effective in protecting against radiation-induced damages in rat testis, and thereby may improve the quality of patient's life after the therapy.en_US
dc.identifier.doi10.1007/s00204-008-0382-y
dc.identifier.endpage746en_US
dc.identifier.issn0340-5761
dc.identifier.issn1432-0738
dc.identifier.issue8en_US
dc.identifier.pmid19015832en_US
dc.identifier.scopus2-s2.0-68149098462en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage735en_US
dc.identifier.urihttps://doi.org/10.1007/s00204-008-0382-y
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21286
dc.identifier.volume83en_US
dc.identifier.wosWOS:000268543300001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofArchives Of Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGamma-Radiationen_US
dc.subjectL-Carnitineen_US
dc.subjectTestisen_US
dc.subjectRaten_US
dc.subjectBlind Randomized-Trialen_US
dc.subjectL-Acetylcarnitine Lacen_US
dc.subjectMouse Spermatogenesisen_US
dc.subjectEpididymal Spermen_US
dc.subjectSeminal Plasmaen_US
dc.subjectFlow-Cytometryen_US
dc.subjectGerm-Cellen_US
dc.subjectIn-Vitroen_US
dc.subjectSertolien_US
dc.subjectRecoveryen_US
dc.titleRole of L-carnitine in the prevention of seminiferous tubules damage induced by gamma radiation: a light and electron microscopic studyen_US
dc.typeArticleen_US

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