Effects of Urtica dioica on oxidative stress, proliferation and apoptosis after partial hepatectomy in rats
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Sage Publications Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The present study was performed to investigate the effect of Urtica dioica (UD) on liver regeneration after partial hepatectomy (PH) in rats. A total of 24 male Sprague Dawley rats were divided into three groups: sham-operated, PH and PH+UD; each group contains eight animals. The rats in UD-treated groups were given UD oils (2ml/kg/day) once a day orally for 7days starting 3days prior to hepatectomy operation. At day 7 after resection, liver samples were collected. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) were estimated in liver homogenates. Moreover, histopathological examination, mitotic index (MI), proliferating cell nuclear antigen labeling, proliferation index (PI), transferase-mediated deoxyuridine triphosphate nick end-labeling assay, apoptotic index (AI) were evaluated at day 7 after hepatectomy. As a result, UD significantly increased MI and PI, significantly decreased AI and also attenuated hepatic vacuolar degeneration and sinusoidal congestion in PH rats. UD treatment significantly decreased the elevated tissue MDA level and increased the reduced SOD activity and GSH level in the tissues. These results suggest that UD pretreatment was beneficial for rat liver regeneration after partial hepatectomy.
Açıklama
Anahtar Kelimeler
UD, Partial Hepatectomy, Oxidative Stress, Immunohistochemistry, TUNEL, Rat, Cell Nuclear Antigen, Liver-Regeneration, Lipid-Peroxidation, Hepatocellular-Carcinoma, Superoxide-Dismutase, Growth-Factor, Vitamin-E, Antioxidant, Hepatocyte, Pathology
Kaynak
Toxicology And Industrial Health
WoS Q Değeri
Q2
Scopus Q Değeri
Q3
Cilt
31
Sayı
5
