Determination of some properties of free and immobilized urease from Aspergillus fumigatus and its application in urea assay
No Thumbnail Available
Date
2016
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Chemical Society of Pakistan
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
Urease enzyme was extracted from Apergillus fumigatus and immobilized in calcium alginate beads. The immobilization efficiency was calculated as 82.5 %. Optimum pH and temperature for free and immobilized enzymes were found to be 7.0 and 40°C, respectively. The immobilized urease had a better Km value but, catalytic efficiencies (kcat/Km) were very similar. Immobilized enzyme maintained 44% of its initial activity after 5 repeated use of enzyme. It was found that storage stability of immobilized enzyme was better than that of the free enzyme. Immobilized urease enzyme was used for the determination of urea amounts in animal feed. © 2016, Chemical Society of Pakistan. All rights reserved.
Description
Keywords
Animal Feed; Aspergillus Fumigatus; Calcium Alginate; Immobilization; Urease
Journal or Series
Journal of the Chemical Society of Pakistan
WoS Q Value
Scopus Q Value
Q4
Volume
38
Issue
3
