Ecarin clotting time is sensitive to heparinoids: Comparison of two different techniques

Basit Öğe Kaydı
dc.contributor.authorDemir, M
dc.contributor.authorIqbal, O
dc.contributor.authorUntch, B
dc.contributor.authorHoppensteadt, DA
dc.contributor.authorGaikwad, BS
dc.contributor.authorFareed, J
dc.date.accessioned2024-06-12T11:03:07Z
dc.date.available2024-06-12T11:03:07Z
dc.date.issued2001
dc.departmentTrakya Üniversitesien_US
dc.description.abstractEcarin clotting time (ECT) is currently developed for the specific monitoring of antithrombin drugs, such as hirudin, argatroban, and hirulog. Aqueous reagent and dry chemistry technology have become available for ECT monitoring of antithrombin agents. Currently, many heparinoids and heparinomimetic drugs are being developed. These agents activate heparin cofactor II (HCII) and primarily mediate their effects by inhibiting thrombin. Although the test is specific for antithrombin agents, heparin cofactor II-mediated thrombin inhibitors are capable of prolonging the ECT. In order to study the relative effects of some of these agents, ECT was measured in human plasma supplemented with PI-88 (a sulfated pentomanose; Progen Industries Limited, Sydney, Australia), aprosulate, pentosan polysulfate, dermatan sulfate, unfractionated heparin (UFH), and recombinant hirudin (r-hirudin). All agents were supplemented to the citrated-pooled plasma prepared from 10 healthy volunteers at a graded dosage of 0 to 100 mug/ml. These techniques gave comparable results for all of the agents used (PI-88, r(2) = 0.99; r-hirudin, r(2) = 0.98; UFH, r(2) = 0.98; dermatan sulfate, r(2) = 0.95; aprosulate, r(2) = 0.95; pentosan polysulfate, r(2) = 0.94). The relative anticoagulant effects of various agents used on ECT varied widely, exhibiting their potency in the following order: r-hirudin = pentosan polysulfate > dermatan sulfate > PI-88 > aprosulate > UFH. The sensitivity of ECT was adjusted by varying the concentration of the ecarin reagent. The results suggest that HCII-mediated inhibition of thrombin can be detected by using ECT reagents.en_US
dc.identifier.doi10.1177/107602960100700109
dc.identifier.endpage43en_US
dc.identifier.issn1076-0296
dc.identifier.issue1en_US
dc.identifier.pmid11190903en_US
dc.identifier.scopus2-s2.0-0035143010en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage38en_US
dc.identifier.urihttps://doi.org/10.1177/107602960100700109
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21543
dc.identifier.volume7en_US
dc.identifier.wosWOS:000166031700009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.ispartofClinical And Applied Thrombosis-Hemostasisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHeparinoidsen_US
dc.subjectEcarin Clotting Timeen_US
dc.subjectHeparin Cofactor IIen_US
dc.subjectPI-88en_US
dc.subjectRecombinant Hirudinen_US
dc.subjectHirudinen_US
dc.subjectAnticoagulanten_US
dc.titleEcarin clotting time is sensitive to heparinoids: Comparison of two different techniquesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu