Transcriptomic effects of Aspergillus alliaceus on Orobanche during its pathogenesis

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dc.contributor.authorAybeke, Mehmet
dc.date.accessioned2024-06-12T11:11:45Z
dc.date.available2024-06-12T11:11:45Z
dc.date.issued2018
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe aim of this study was to investigate the pathogenicity of Aspergillus alliaceus (Aa), a fungal bio-control agent, on Orobanche by transcriptomic tests. For this reason, the expression of several genes was analysed comparatively in the experimental (fungi applied) and control (fungi-free) groups. After fungal infection, the expression of antioxidant enzyme genes SOD (Mn-super-oxide dismutase), CAT (catalase), and SOD2 (Zn-super-oxide dismutase) and the genes GS (Glutamine synthetase), HSP70 (heat-shock protein), and apoptosis [BCL2, BAX, caspase3 (CASP3)], which are related to protein metabolism, were investigated in the both groups. In gene expression studies, gene expression of SOD was almost 2.5 times higher in the experimental group than in the control group (fungus-free, intact), while the expression levels of other antioxidant genes (CAT and SOD2) were significantly reduced. While GS, which is the N and protein metabolism balancer, was found to be relatively low as compared to the control group, however, the level of HSP70 (protein folding, chaperoning) was quite higher than the control. Gene expressions of all apoptotic genes, BAX (=apoptosis suppressive gene), CASP3 and BCL (=apoptosis inducer genes), were significantly lower than control group. All the results found for the control group were determined to be statistically significant. It was concluded that during Aa pathogenesis, (1) the increased SOD value was associated with ROS (reactive oxygen species) threats, (2) the fungi disturbed protein synthesis metabolism, and finally, (3) the inhibited antioxidant and apoptosis-based pathways compared to the control group. These conclusions were discussed in the light of slow but constantly lethal effects of Aa comparatively to other pathogenic fungus, Fusarium.en_US
dc.description.sponsorshipTUBAP (Trakya University, Scientific Researches Foundations) [TUBAP 2014-82]en_US
dc.description.sponsorshipI thank Prof. Dr. Oguzhan DOGANLAR for his valuable comments and TUBAP (Trakya University, Scientific Researches Foundations) with the Project Number TUBAP 2014-82.en_US
dc.identifier.doi10.1007/s41348-017-0122-8
dc.identifier.endpage39en_US
dc.identifier.issn1861-3829
dc.identifier.issn1861-3837
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85040766167en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage33en_US
dc.identifier.urihttps://doi.org/10.1007/s41348-017-0122-8
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22906
dc.identifier.volume125en_US
dc.identifier.wosWOS:000429380100005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofJournal Of Plant Diseases And Protectionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOrobancheen_US
dc.subjectBroomrapeen_US
dc.subjectGen Expressionen_US
dc.subjectAntioxidanten_US
dc.subjectApoptosisen_US
dc.subjectBiocontrolen_US
dc.subjectAbiotic Stress Toleranceen_US
dc.subjectBiological-Controlen_US
dc.subjectFusarium-Oxysporumen_US
dc.subjectPhytochemical Analysisen_US
dc.subjectArabidopsis-Thalianaen_US
dc.subjectCell-Deathen_US
dc.subjectAntioxidanten_US
dc.subjectInfectionen_US
dc.subjectEfficacyen_US
dc.subjectOrthocerasen_US
dc.titleTranscriptomic effects of Aspergillus alliaceus on Orobanche during its pathogenesisen_US
dc.typeArticleen_US

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