Immobilization of Aspergillus oryzae ?-Galactosidase on Low-pressure Plasma-modified Cellulose Acetate Membrane Using Polyethyleneimine for Production of Galactooligosaccharide

Basit Öğe Kaydı
dc.authoridAlbayrak, Nedim/0000-0002-6356-5805
dc.authoridMUTLU, MEHMET/0000-0001-9610-9817
dc.authoridMutlu, Mehmet/0000-0001-7146-1937
dc.authorwosidAlbayrak, Nedim/AFA-5818-2022
dc.authorwosidMUTLU, MEHMET/B-3240-2013
dc.authorwosidMutlu, Mehmet/S-5351-2019
dc.contributor.authorGulec, Haci Ali
dc.contributor.authorGurdas, Sevim
dc.contributor.authorAlbayrak, Nedim
dc.contributor.authorMutlu, Mehmet
dc.date.accessioned2024-06-12T11:00:28Z
dc.date.available2024-06-12T11:00:28Z
dc.date.issued2010
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe aim of this study was to produce galacto-oligosaccharides (GOS) from lactose using beta-galactosidase from Aspergillus oryzae immobilized on a low-pressure plasma-modified cellulose acetate (CA) membrane Specifically, a novel method was developed for multilayer enzyme immobilization involving polyethyleneimine (PEI)-enzyme aggregate formation and growth on a CA membrane A large amount of enzyme (997 mu g/cm(2) membrane) was immobilized with 66% efficiency The K(m) value for the immobilized enzyme was estimated to be 48 mM, which indicates decreased affinity for the substrate, whereas the Vmax value was smaller The immobilized enzyme showed good storage and operational stability The half-life of the immobilized enzyme on the membrane was about 1 month at 30 degrees C and similar to 60 h at 60 degrees C Maximum GOS production of 27% (w/w) was achieved with 70% lactose conversion from 320 g/L of lactose at pH 4 5 and 60 degrees C Trisaccharides were the major types of GOS formed and accounted for about 75% of the total GOS produced Based on these results, immobilized enzyme technology could be applied to GOS production from lactoseen_US
dc.description.sponsorshipYuzuncu Yil University [2008-FBE-D011]; Scientific and Technological Research Council of Turkey [1060116]en_US
dc.description.sponsorshipThis work was supported in part by the Scientific Research Fund of Yuzuncu Yil University (No 2008-FBE-D011) and The Scientific and Technological Research Council of Turkey (Grant No 1060116)en_US
dc.identifier.doi10.1007/s12257-010-0046-7
dc.identifier.endpage1015en_US
dc.identifier.issn1226-8372
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-79952669000en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1006en_US
dc.identifier.urihttps://doi.org/10.1007/s12257-010-0046-7
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20850
dc.identifier.volume15en_US
dc.identifier.wosWOS:000285862900016en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherKorean Soc Biotechnology & Bioengineeringen_US
dc.relation.ispartofBiotechnology And Bioprocess Engineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnzyme Immobilizationen_US
dc.subjectPolyethyleneimineen_US
dc.subjectLow-Pressure Plasma Polymerizationen_US
dc.subjectCellulose Acetate Membraneen_US
dc.subjectGalactooligosaccharideen_US
dc.subjectBacillus-Circulansen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectOligosaccharidesen_US
dc.subjectLactoseen_US
dc.subjectPolymerizationen_US
dc.subjectHydrolysisen_US
dc.subjectSeparationen_US
dc.subjectReactoren_US
dc.subjectSurfaceen_US
dc.subjectBeadsen_US
dc.titleImmobilization of Aspergillus oryzae ?-Galactosidase on Low-pressure Plasma-modified Cellulose Acetate Membrane Using Polyethyleneimine for Production of Galactooligosaccharideen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu