Ultrasensitive Impedimetric Biosensor Fabricated by a New Immobilisation Technique for Parathyroid Hormone

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dc.authoridAYDIN, TUBA/0000-0002-7653-6480
dc.authoridAsav, Engin/0000-0002-6232-3388;
dc.authorwosidAYDIN, TUBA/W-4227-2017
dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.authorwosidAsav, Engin/AAG-1170-2021
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorOzcan, Hakki Mevlut
dc.contributor.authorYildiz, Kubra
dc.contributor.authorCakar, Cansu
dc.contributor.authorAydin, Tuba
dc.contributor.authorAsav, Engin
dc.contributor.authorSagiroglu, Ayten
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2024-06-12T11:12:04Z
dc.date.available2024-06-12T11:12:04Z
dc.date.issued2015
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThis paper presents a novel ultrasensitive and rapid impedimetric biosensor with new immobilisation materials for parathyroid hormone (PTH) with the aim to determine the PTH level in serum for the diagnosis and monitoring of parathyroid diseases such as hyperparathyroidism, adenoma, and thyroid cancer. The interaction between PTH and the biosensor was investigated with an electrochemical method. The biosensor was based on the gold electrode modified by mercaptohexanol (6-MHL). Anti-parathyroid hormone (anti-PTH) was covalently immobilised onto a self-assembled monolayer (SAM) by using epiclorhidrina (EPI) with ethanolamine (EA). The EPI-EA interaction represents the first use of these for the construction of biosensors in published reports. The immobilisation of the anti-PTH was monitored by electrochemical impedance spectroscopy, cyclic voltammetry and scanning electron microscopy (SEM) techniques. After the optimisation studies of immobilisation materials such as 6-MHL, EPI, EA and glutaraldehyde, linearity, repeatability and sensitivity of biosensor were evaluated as the performance of biosensor. PTH was detected within a linear range of 0.1-0.6 pg/ml, and the detection limit was 0.1 fg/ml. The specificity of the biosensor was also investigated. Finally, the described biosensor was used to detect the PTH levels in artificial serum samples.en_US
dc.description.sponsorshipTrakya University Scientific Research Foundation [TUBAP-2001/195]; TUBITAK (The Scientific and Technological Research Council of Turkey) [113 Z 678]en_US
dc.description.sponsorshipSupport from the Trakya University Scientific Research Foundation (project number: TUBAP-2001/195) and TUBITAK (The Scientific and Technological Research Council of Turkey; project number: 113 Z 678) are greatly acknowledged.en_US
dc.identifier.doi10.1007/s12010-015-1643-x
dc.identifier.endpage1262en_US
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.issue5en_US
dc.identifier.pmid25935225en_US
dc.identifier.scopus2-s2.0-84937971512en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1251en_US
dc.identifier.urihttps://doi.org/10.1007/s12010-015-1643-x
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23033
dc.identifier.volume176en_US
dc.identifier.wosWOS:000358379400002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofApplied Biochemistry And Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectParathyroid Hormoneen_US
dc.subjectBiosensoren_US
dc.subjectEpiclorhidrinaen_US
dc.subjectElectrochemical Impedance Spectroscopyen_US
dc.subject6-Mercaptohexanolen_US
dc.subjectSelf-Assembled Monolayersen_US
dc.subjectImpedance Biosensoren_US
dc.subjectProteinen_US
dc.subjectSamen_US
dc.titleUltrasensitive Impedimetric Biosensor Fabricated by a New Immobilisation Technique for Parathyroid Hormoneen_US
dc.typeArticleen_US

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