The therapeutic effects of transferring remote ischemic preconditioning serum in rats with neuropathic pain symptoms

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dc.authoridKaya, Oktay/0000-0001-9639-8022
dc.authoridGunduz, Ozgur/0000-0002-2470-3021
dc.authoridOrhan, Abdullah Erkan/0000-0001-6532-5840
dc.authoridUlugol, Ahmet/0000-0003-4643-1124
dc.authorwosidKaya, Oktay/JGL-9169-2023
dc.authorwosidGunduz, Ozgur/A-2351-2016
dc.authorwosidOrhan, Abdullah Erkan/A-6237-2017
dc.contributor.authorGunduz, Ozgur
dc.contributor.authorYurtgezen, Zekiye Gulfem
dc.contributor.authorTopuz, Ruhan Deniz
dc.contributor.authorSapmaz-Metin, Melike
dc.contributor.authorKaya, Oktay
dc.contributor.authorOrhan, Abdullah Erkan
dc.contributor.authorUlugol, Ahmet
dc.date.accessioned2024-06-12T11:09:11Z
dc.date.available2024-06-12T11:09:11Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground and objectives: Neuropathic pain is defined as pain caused by damage to the nerve as a result of a lesion or disease. It has been shown that ischemic preconditioning exerts a protective role in various tissue injuries; however, the effect of transplantation of remote ischemic preconditioning serum (RIPCs) on neuropathic pain symptoms has not been studied. The aim of this project is to investigate the effect of RIPCs transfusion by different routes of administration on neuropathic pain symptoms. Our secondary aim was to demonstrate the role of Schwann cells in the regeneration of sciatic nerve injury and to evaluate the change in the number of glial cells in the spinal cord dorsal horn.Methods: The sciatic nerve partial ligation method was used to induce neuropathic pain. Changes in neuropathic pain symptoms were assessed by measuring thermal hyperalgesia and mechanical allodynia. To determine the possible therapeutic site, alterations in the number of spinal cord lumbar posterior horn microglia and astrocytes were evaluated by ionized calcium-binding adapter molecule 1 (iba1) and glial fibrillary acidic protein (GFAP) immunostaining. Myelin basic protein immunohistochemistry was also used to assess Schwann cell immunoreactivity in the sciatic nerve.Results: In rats that underwent partial sciatic nerve ligation, neuropathic pain symptoms developed on average on day 12 and persisted up to day 21 (p < 0.0001). RIPCs administered intravenously for five days reduced thermal hyperalgesia more than intraperitoneal and subcutaneous administration (p < 0.05). Both central glial cells appear to play a role in the effect of RIPCs. RIPCs treatment increases Schwann cell remyelination.Conclusions: Our results showed that intravenously administered RIPCs remarkably improved the neuropathic pain symptoms, thermal hyperalgesia and mechanical allodynia. Further studies are needed to evaluate the role of RIPCs transfusion on glial cells.en_US
dc.description.sponsorshipTrakya University Research Council [TUBAP-2020/111]en_US
dc.description.sponsorshipFunding his work was supported by a grant from Trakya University Research Council (TUBAP-2020/111) .en_US
dc.identifier.doi10.1016/j.heliyon.2023.e20954
dc.identifier.issn2405-8440
dc.identifier.issue10en_US
dc.identifier.pmid37867836en_US
dc.identifier.scopus2-s2.0-85173696469en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.heliyon.2023.e20954
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22700
dc.identifier.volume9en_US
dc.identifier.wosWOS:001114647100001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCell Pressen_US
dc.relation.ispartofHeliyonen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNeuropathic Painen_US
dc.subjectRemote Ischemic Preconditioningen_US
dc.subjectHyperalgesiaen_US
dc.subjectAllodyniaen_US
dc.subjectGlial Cellsen_US
dc.subjectTactile Allodyniaen_US
dc.subjectSpinal Microgliaen_US
dc.subjectNerve Injuryen_US
dc.subjectAstrocytesen_US
dc.subjectModelsen_US
dc.subjectCellsen_US
dc.subjectGliaen_US
dc.titleThe therapeutic effects of transferring remote ischemic preconditioning serum in rats with neuropathic pain symptomsen_US
dc.typeArticleen_US

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