Synthesis of novel dimeric compounds containing triazole using click method and their selective antiproliferative and proapoptotic potential via mitochondrial apoptosis signaling
Küçük Resim Yok
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Birkhauser
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, the main aim was synthesis of dimeric compounds, which contain lithocholic acid and a triazole structure to investigate the selective cellular and molecular antiproliferative and proapoptotic potential of these products in healthy embryonic fibroblast (MEF), cervix cancer (HeLa), and breast cancer (MCF-7) cells. Four ester (5a-d) and five dimeric (6a-d, 7) out of nine novel compounds were obtained. First of all, lithocholic acid was converted to methyl lithocholate and then it was reacted with certain alkynoic acids (a-d) to obtain its alkynoate derivatives (5a-d). Finally, these compounds were converted to dimers (6a-d) by using 2,6-bis(azidomethyl)pyridine via the click method. Our result indicate that, treatment with dimeric compounds can selectively decrease the cell viability and proliferation in cervix cancer HeLa and breast cancer MCF-7 cells, except 7 which caused a strong cytotoxicity on healthy MEF cells. According to MTT assay, Nucblue cell stain and Annexin V/Propodium iodide molecular probe staining, 100 mu M concentrations of the dimeric compounds was sufficient in inducing death and apoptotic cell ratio in HeLa and MCF-7 breast cancer cells selectively. In brief, the present study indicates that most effective dimeric compounds are 6a and 6b, which have the highest IC50 (345.8-342.6 mu M) value on healthy cell and the lowest IC50 value in both cervix (49.2-36.9 mu M) and breast (23.0-66.1 mu M) cancer cells especially long-term treatment and which triggers apoptosis pathway specifically.
Açıklama
Anahtar Kelimeler
Click Chemistry, Lithocholic Acid, Hela, MCF-7, Gene Expression, Lithocholic Acid, Derivatives, Chemistry, Activation, Therapy, Analogs, Design, Cells, Hiv
Kaynak
Medicinal Chemistry Research
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
29
Sayı
4
