Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells

Basit Öğe Kaydı
dc.contributor.authorGrıgorova, Natalia
dc.contributor.authorCza, Elen G
dc.contributor.authorVachkova, Ekaterina
dc.date.accessioned2024-06-12T10:13:13Z
dc.date.available2024-06-12T10:13:13Z
dc.date.issued2020
dc.departmentTrakya Üniversitesien_US
dc.description.abstractCardiovascular diseases are still one of the most common reasons for mortality in humans. Mesenchymal stem cells (MSCs) are preferable in cardiac regeneration cell-based therapies because of their allogeneic and high proliferative potential. The electrophysiological properties of the rabbit heard is closer to human than the mouse. The current study aimed to trace mRNA expression changes of two stemness/cardiogenic differentiation ability-related transcriptionala factors OCT4 and GATA4 in rabbit MSCs during early stages of induced cardiomyocyte differentiation in vitro. The mesenchymal stem cell originated from different anatomical areas-subcutaneous, visceral, bone marrow and pericardial tissue. The cardiac differentiation protocol for mouse embryonic stem cells in hanging drop was adopted in rabbit MSCs. The best formed embryonal bodies (EBs) like structures were collected and cultivated on gelatin-coated plates. The total mRNA was obtained before cardiac differentiation and on the 6th day after it. SYBER based real-time PCR was performed to evaluate the mRNA expression fold-changes of OCT4 and GATA4. The cultivation of MSCs in hanging drops during cardiac differentiation induced EBs formation, without any contractile activity up to the 6th day of the differentiation in all cell types. The applied differentiation protocol significantly downregulated GATA4 expression in ADSCs - EBs, while in BMSCs, both target genes were significantly upregulated. In conclusion, the adopted cardiac differentiation protocol from mouse embryonic stem cells could be a useful approach for rabbit bone marrow mesenchymal stem cells. Since the rest of the cells revealed weak cardiogenic capability at this early stage, some modifications of induction protocols should be considered.en_US
dc.identifier.endpage412en_US
dc.identifier.issn1300-0861
dc.identifier.issn1308-2817
dc.identifier.issue4en_US
dc.identifier.startpage407en_US
dc.identifier.trdizinid1109838en_US]
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1109838
dc.identifier.urihttps://hdl.handle.net/20.500.14551/13860
dc.identifier.volume67en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofAnkara Üniversitesi Veteriner Fakültesi Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titlePilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cellsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu