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Öğe An Investigation on the Anticancer Effect of Spider Web in Human Cervical Cell Line(Trakya Üniversitesi, 2019) Özkan, Aslı Nur; Doğanlar, OğuzhanAims: The aim of this study is to investigate the anti-cancer and anti-proliferative effect of spider web extract, and mitochondrial apoptosis signaling response via gene expression levels belonging to caspase-3 depended apoptosis in both healthy Astrocyte and cancerous HeLa cell lines. Methods: We used HeLa and C8-D1A Astrocyte type I clone cell lines as the human cervical cancer and healthy cell line, respectively. We treated the cells with spider web extract and performed MTT assay. IC50 was calculated by probit analysis. Molecular fluorescence staining and TALI cytometer measurements were also applied. The response of the mitochondrial apoptosis pathway to spider web extract treatment was analyzed by the qRT-PCR assay. Results: The spider web extract administered in different concentrations to the HeLa cell line reduced cell viability at a statistically significant level after 24 hours of application. In the astrocyte cell line, the spider web extract did not cause statistically significant cell death. In the analysis of gene expressions, a strong mitochondrial apoptosis signaling was observed. Conclusion: While spider web extract does not cause any cytotoxic effects on healthy cell lines, it causes a strong fatal effect in cancer cell line. In addition, IC50 dose of the spider web extract is satisfying compared to many other natural products in the market which have anti-cancer effects. Spider web extract causes a programmed cell death by following death signaling pathwaysÖğe The Investigation of University Students’ Knowledge on Nutrition and Eating Habits(Trakya Üniversitesi, 2018) Koçak, Ertuğrul; Parlak, Atakan Muhammet; Kızılkaya, Orkun Salih; Bardakçı, Burak; Kılıç, Musa Eralp; Eskiocak, SevgiAims: The aim of this study is to analyze Trakya University School of Medicine students’ knowledge on nutrition and their eating habits. In addition, it is aimed to determine whether there is a relation between knowledge and nutrition habits and whether medical education is enough to learn the correct information. Methods: The study conducted in May 2018 was carried out by questionnaires given to 681 students, which were composed of 240 first graders, 98 second graders, 78 third graders, 143 fourth graders, and 110 fifth graders. Chi-square method was used to determine the relation between the class and the gender. Results: Out of all students 30.4% of them thought that it is harmful to eat fish and yogurt together. 80.3% of them had the idea that consuming calcium in their diet does not increase iron absorption. 66.9% of the participants declared that they drink tea without sugar.Conclusion: It was determined that the students do not apply the information they know correctly to their daily lives. In some questions, it was observed that the students’ knowledge about nutrition is insufficient. In order to correct the eating habits and to create public awareness of nutrition, courses about nutrition should be included in the curriculum of medical students.Öğe The Effect of 5-Fu and Ruxolitinib on Mitochondrial Apoptosis in Glioblastoma U87 Cell Line(Trakya Üniversitesi, 2020) Aksu, Gonca; Doğanlar, Oğuzhan; Doğanlar, Zeynep BanuAims: The aim of this study is to carry out the effect of 5-Fluorouracil alone or combined with Ruxolitinib on both apoptosis and JAK/STAT pathway in U87 glioblastoma cells. Methods: We used U87 glioblastoma cell lines as the human brain cancer cells. We treated the cells with 5-Fluorouracil (3.125 ?M-400 ?M) alone and with a combination of Ruxolitinib (100 ?M or 400 ?M of Ruxolitinib with 3.125-25 ?M 5-Fluorouracil), and performed the MTT test for calculating IC50 value. Molecular fluorescence staining was performed with Hoechst and acridine orange/ethidium bromide probes. The alteration in mitochondrial apoptosis and JAK/STAT pathways to drug treatment was analyzed by the qRT-PCR assay. Results: Decrease in cell viability was more prominent in U87 cells treated with a combination of 5-Fluorouracil and Ruxolitinib compared to those treated with 5-Fluoro- uracil alone. In gene expression analysis, apoptosis signals were observed in cells treated with 5-Fluorouracil alone and 5-Fluo- rouracil+Ruxolitinib treatment. Conclusion: Treatment with 5-Fluorouracil alone and 5-Fluorouracil+Ruxolitinib combination increased apoptosis in U87 glioblastoma cells. However, it is difficult to mention an evident difference between treatments. Therefore, further studies are needed.Öğe Naringin sensitizes human prostate cancer cells to paclitaxel therapy(Elsevier, 2018) Erdogan, Suat; Doganlar, Oguzhan; Doganlar, Zeynep B.; Turkekul, KaderBackground: The aim of the study was to evaluate whether the use of chemotherapy in combination with naringin, a dietary plant polyphenolic flavonoid, could enhance the therapeutic efficacy of paclitaxel treatment in human prostate cancer (PCa) cells. Materials and methods: DU145, PC3, and LNCaP cells were treated with various concentrations of paclitaxel, naringin, and their combinations. Methylthiazolyldiphenyl-tetrazolium bromide (MTT), image-based cytometer, quantitative reverse transcription PCR (RT-qPCR), Western blot, and transwell assay were used to evaluate cell viability, apoptosis and cell cycle, the mRNA expression, protein expression, and cell migration, respectively. Results: Naringin treatment inhibited cell survival in a dose- and time-dependent manner by inducing apoptosis and cell cycle arrest in G1 phase. Among the pathways evaluated, naringin (150 mu M) significantly induced the mRNA expressions of BAX, BID, caspase 3, cytochrome c, p53, p21(Cip1), and p27(Kip1) and down-regulated the expressions of survivin and livin in DU145 cells. The combination of naringin and paclitaxel treatments synergistically increased the cytotoxic effects of paclitaxel in androgen-independent DU145 and PC3 cells, as well as in androgen-sensitive LNCaP cells. The combination of naringin with docetaxel has almost the same inhibitory effect on cell proliferation as the paclitaxel combination in androgen-independent cells, whereas there is no similar effect in LNCaP cells. Naringin exhibits significant inhibitory effects on the cell migration ability. The flavonoid either alone or in combination with paclitaxel therapy resulted in an increase in tumor suppressor PTEN (phosphatase and tensin homolog deleted on chromosome 10) protein expression and decrease in nuclear factor-kB p50 protein level in DU145 cells. Conclusion: In conclusion, naringin acts as a chemosensitizer which synergistically strengths the cytotoxic effect of paclitaxel in PCa cells. Therefore, naringin therapy alone or in combination with paclitaxel may be useful in the treatment of PCa. However, there is a need for more detailed in vivo studies of the mechanism of action. (C) 2017 Asian Pacific Prostate Society, Published by Elsevier Korea LLC.
