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    Genetic diversity in sodium azide induced wheat mutants studied by ssr markers
    (Trakya Üniversitesi Fen Bilimleri Enstitüsü, 2018) Şen, Ayşe; Sarsu, Fatma
    Mutationsinduced artificially way are one of the tools used to increase genetic variation in populations where genetic variation has been shrinking especially due to various reasons one of which is domestication. In this study, Simple Sequence Repeats (SSRs) markers were used to screen genetic diversity in sodium azide (NaN3) induced fourteen fourth-generation advanced wheat mutant lines. The mean values of polymorphism rate (29.44%), polymorphic information content (PIC; 0.82), marker index (MI; 1.95) and resolving power (Rp; 1.31) were calculated according to SSR marker profiles. Two SSRs, Xwmc170 and Xcfd6, were detected as the most polymorphic markers, Xgwm626 proved the highest PIC and MI values, and Xcfd6 gave the highest Rp value. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrogram classified 15 plants into four groups. The Principle Component Analysis (PCA) showed 88.9% of the total genetic variation. The results obtained in the present study might be useful for determining the efficiency of NaN3 for creating mutant wheat lines with enough genetic variability to implement wheat-breeding programs as germplasm resources.
  • Küçük Resim
    Öğe
    Obtaining candidate salt tolerant wheat mutant lines derived from combination of sodium azide mutagenesis and somatic embryogenesis
    (Trakya Üniversitesi Fen Bilimleri Enstitüsü, 2019) Şen, Ayşe; Sarsu, Fatma
    Plant mutants are important bio-resources for crop breeding and functional gene studies. In the present study, conventional chemical mutagenesis technique was combined with somatic embryogenesis to obtain candidate salt tolerant mutant wheat lines. For this purpose, 0-5 mM Sodium Azide (NaN3) was applied for 30 minutes to embryonic calli under in vitro conditions to produce genetic variations in the bread wheat (Triticum aestivum L. cv. Adana 99). Treated and non-treated calli were put in somatic embryo induction media, and 3 and 4 mM NaN3 were determined as optimum mutation doses for somatic embryo induction. The obtained somatic embryos from these optimum mutagen doses were then screened for tolerance in regeneration media containing 125 mM NaCl to be used to improve tolerance to salt stress. In NaN3 treatment, 14 mutants with moderate salt tolerance were obtained. The results suggest that the in vitro technique in combination with chemical mutagenesis may be a useful approach for accelerating breeding strategies to create enough genetic variation in populations and to get fourth generation putative salt tolerant wheat mutant lines in less than 1.5 years.