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    Chromosome aberrations, micronucleus and sperm head abnormalities in mice treated with Delvocid, a food preservative (vol 48, pg 789, 2010)
    (Pergamon-Elsevier Science Ltd, 2010) Rasgele, Pinar Goc; Kaymak, Fisun
    [Abstract Not Available]
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    Evaluation of Genotoxic and Cytotoxic Effects of Natamycin in Mice Bone Marrow Cells
    (Zoological Soc Pakistan, 2013) Rasgele, Pinar Goc; Kaymak, Fisun
    Natamycin, food preservative, is widely used in food industry against yeast and fungi. The potential genotoxicity of a commercial formulation of natamycin (Delvocid, containing 50% natamycin as the active ingredient) in mice bone marrow cells Was investigated in vivo by chromosome aberrations (CA) and micronucleus assays (MA). Animals were intraperitoneally treated with 200, 400 and 800 mg/kg of natamycin for 6, 12 and 24 h in CA assay, for 24, 48 and 72 hours in MN assay. In the present study, natamycin did not increase chromosome aberrations. It significantly induced frequency of micronucleus at all concentrations for both 24 and 48 h in female mice, at the two highest (400 and 800 mg/kg) concentrations for both 24 and 48 h in male mice when compared with negative control. Mice bone marrow erythrocytes exposed to all concentrations of natamycin showed significant. decreases in mitotic index for all treatment periods. In addition, natamycin reduced polychromatic erytrocyte/normochromatic erytrocyte ratio at all concentrations for 48h and at the highest (800 mg/kg) concentration for 24 and 72h in female mice; at all concentrations for 24 and 48h in male mice. The results of the present study show that a commercial formulation of natamycin was aneugenic and cytotoxic to mice bone marrow in vivo. For this reason it is necessary to be careful when using these chemicals in food as preservatives.
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    Genotoxic Effects of Raxil on Root Tips and Anthers of Allium cepa L.
    (Firenze Univ Press, 2009) Fisun, Kaymak; Rasgele, Pinar Goc
    The genotoxic effects of fungicide Raxil, active substance tebuconazole in both mitotic and meiotic cell divisions of Allium cepa L. were studied. The bulbs with roots of Allium cepa L. were treated with different concentrations of Raxil (1800 ppm, 2400 ppm, 4200 ppm, 6000 ppm) for 3, 6, 12 and 24 hours. For mitotic studies, the root tips of Allium cepa L. after having grown to a certain length were stained accoording to aceto orcein squash procedure. To determine the effects of Raxil on meiotic cell division was used M1 generation. All concentrations and treatment periods of Raxil induced a number of chromosomal aberrations in root tip cells and in anthers of Allium cepa L. Additionally Raxil decreased the frequency. of mitotic index and caused pollen fertility. A linear relationship was observed between increase of chromosomal abnormality with decrease of mitotic index and pollen fertility.
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    Induction of Micronuclei in Bone Marrow Cells of Mice Exposed to Acetamiprid
    (Springer, 2013) Rasgele, Pinar Goc; Muranli, Fulya Dilek Gokalp; Kekecoglu, Meral
    [Abstract Not Available]
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    INDUCTION OF MICRONUCLEI IN MICE BONE MARROW CELLS BY COBALT AND COPPER CHLORIDES
    (Polska Akad Nauk, Polish Acad Sciences, Inst Environ Eng Pas, 2013) Rasgele, Pinar Goc; Kekecoglu, Meral; Muranli, Fulya Dilek Gokalp
    The aim of our research was to investigate the genotoxic effects of cobalt chloride and copper chloride in mouse bone marrow cells using the micronucleus (MN) assay. The three different concentrations of cobalt chloride (11.2, 22.5 and 45 mg kg(-1)) and copper chloride (1.17, 2.35 and 4.70 mg kg(-1)) were injected intraperitoneally to mice for 24 and 48 hours. It was observed that both of these heavy metals induced a significant increase in frequency of micronucleated polychromatic erythrocytes (MNPCE) at different concentrations in mice for 24 and 48 hours when compared with the control. Furthermore, the significant reduction for the polychromatic erythrocyte/normochromatic erythrocyte (PCE/NCE) ratio which is indicative of bone marrow cytotoxicity was observed in bone marrow cells which were treated with copper chloride at all concentrations for 24 and 48 hours. No reduction of the PCE/NCE ratio was observed both 24 and 48 hours after all the doses of cobalt chloride tested as compared to the negative control. These results lead us to the conclusion that copper chloride may have genotoxic and cytotoxic properties due to induction in the frequency of MN and a reduction in PCE/NCE ratio in bone marrow cells of mice, whereas cobalt chloride induced only genotoxic effect in mice bone marrow.
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    Investigation of genotoxic effects of rhododendron honey using three mammalian bioassays in vivo
    (Taylor & Francis Ltd, 2022) Rasgele, Pinar Goc; Gokalp, Fulya Dilek; Kaya, Salih Tunc; Kekecoglu, Meral; Acar, Merve Kambur
    Rhododendron honey (RH) is obtained from the rhododendron plants are grown in many regions around the world, causes poisoning in humans due to the grayanotoxin (GTX) compound in its structure. It is used by the public as a therapeutic for some diseases. It was aimed to study the genotoxic and cytotoxic effects of RH in mouse bone-marrow and sperm cells by using three mammalian bioassays. 25, 50 and 75 mg kg(-1) concentrations of RH given to male mice via gavage for 24 and 48 h treatment periods and its active ingredient Grayanatoxin (GTX-III) 0.01 mg kg(-1) by i.p. injection. Chromosome aberrations (CA), polychromatic erythrocytes (PCE)/normochromatic erythrocytes (NCE), micronucleated polychromatic erythrocytes (MNPCE) and sperm abnormalities were investigated. The results demonstrated that all the tested concentrations of RH significantly induced total abnormal cell frequency including chromosomal breaks for two time periods. In the MN assay, 75 mg kg(-1) RH and 0.01 mg kg(-1) GTX-III significantly increased % MNPCE and significantly reduced PCE/NCE ratios after 24 and 48 h treatments on mice demonstrating potential genotoxic and cytotoxic effect. Although there was a concentration-related increase in the percentage of total sperm abnormalities, this increase was not statistically significant compared to control. As a result, microscopic genotoxicity and cytotoxicity marker tests showed that RH and its active ingredient GTX-III have potential genotoxic and cytotoxic effect on mice bone marrow cells. It is understood that RH that is used to treat some diseases by public, should be handled carefully and used in a controlled manner.
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    POTENTIAL GENOTOXICITY OF ACETAMIPRID AND PROPINEB SINGLY OR IN COMBINATION IN CULTURED HUMAN PERIPHERAL BLOOD LYMPHOCYTES BY USING MN ASSAY
    (Parlar Scientific Publications (P S P), 2015) Muranli, Fulya Dilek Gokalp; Rasgele, Pinar Goc; Kekecoglu, Meral; Kanev, Martin; Ozdemir, Kezban
    Acetamiprid, a neonicotinoid insecticide, and Propineb, a dithicarbamate fungicide, are used in agriculture singly or in combination with other pesticides. The aim of the study is to investigate the single and combined genotoxic effect two pesticides on cultured human lymphocytes by using the Micronucleus (MN) assay. The lymphocytes were treated with three different concentrations of pesticides singly and in combination (a mix), Acetamiprid (0.625, 1.25, 2.5 mu g mL(-1)) Propineb (12.5, 25, 50 mu g mL(-1)) and Acetamiprid+Propineb (0.625+12.5, 1.25+25, 2.5+50 mu g mL(-1)), for 24 and 48 hours. MN frequency significantly increased at a 48-hour exposure period when the lymphocytes were exposed to the combined pesticides. The data demonstrated that these pesticides may have a synergistic effect and may have potential genotoxic activity in cultured human lymphocytes. Also, it was shown that when they are combined and exposed to lymphocytes these pesticides have a cytotoxic effect.