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    Effects of Organophosphorus and Pyrethroid pesticides on antioxidant enzymes and reactivation effects of Pralidoxime:In vitro studies
    (Academic Publication Council, 2022) Paluzarl, Hatice; Sagiroglu, Ayten
    The present study aimed to assess the inhibition effects of organophosphate pesticides, malathion(R), dichlorvos(R); pyrethroid pesticides, deltamethrin(R), lambda-cyhaloethrin(R) on antioxidant enzymes and the reactivation ability of pralidoxime against pesticide inhibited-antioxidant enzymes. Oximes were reported by reactivation ability against organophosphate inhibited-acetylcholinesterase and we focused to investigate the reactivation effect of pralidoxime against organophosphate inhibited-antioxidant enzymes. IC50 values were determined by means of activity percentage diagrams. The concentrations of deltamethrin(R), malathion(R), dichlorvos(R), lambda-cyhaloethrin(R) that inhibited 50% of catalase were 5.2 mu M, 158 mu M, 133 mu M, 320 mu M, respectively, inhibited 50% of superoxide dismutase were 62 mu M, 240 mu M, 328 mu M, 2320 mu M, respectively and inhibited 50% of glutathione peroxidase were 0.7 mu M, 1198 mu M, 1638 mu M, 98 mu M, respectively. All pesticide doses showed an inhibition effect on antioxidant enzymes. Deltamethrin(R) wasfound to be a more potent inhibitor for the antioxidant enzymes followed by the rest of the pesticides used in this study. The reactivation effect of pralidoxime was determined for organophosphate inhibited-enzymes. Reactivation results showed that only catalase is reactivated by pralidoxime against dichlorvos(R) and malathion(R). Under the exposure of 50-800 mu M malathion(R) concentrations, the activities of catalase were calculated as 72-11%, respectively. After, inhibited catalase was incubated with 1 mM and 10 mM pralidoxime, the activities of catalase were calculated as 92-31% and 98-39%, respectively. Under the exposure of 100-1500 mu M dichlorvos(R) concentrations, the activities of catalase were calculated as 50-6%, respectively. After, inhibited catalase was incubated with 1 mM and 10 mM pralidoxime, the activities of catalase were calculated as 95-30% and 93-28%, respectively. When the results are examined, it is seen that increasing the pralidoxime concentration does not significantly affect the reactivation percentage of the catalase enzyme.