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Öğe Association of MG53 with presence of type 2 diabetes mellitus, glycemic control, and diabetic complications(Public Library Science, 2023) Andac, Burak; Ozgun, Eray; Bulbul, Buket Yilmaz; Colak, Serpil Yanik; Okur, Mine; Yekdes, Ali Cem; Ocal, EftalObjectivesMitsugumin 53 (MG53) is a myokine that acts as a membrane repair protein in tissues. Data on the effect of MG53 on insulin signaling and type 2 diabetes mellitus (T2 DM) are still unknown; most are from preclinical studies. Nevertheless, some researchers have argued that it may be a new pathogenic factor, and therapies targeting MG53 may be a new avenue for T2 DM. Our study aims to evaluate the relationship of circulating MG53 levels with the presence of diabetes, diabetic complications, and glycemic control.MethodsWe conducted a case-control study with 107 patients with T2 DM and 105 subjects without insulin resistance-related disease. Concurrent blood samples were used for serum MG53 levels and other biochemical laboratory data. MG53 concentration was measured using Human-MG53, an enzyme-linked immunosorbent assay kit (Cat# CSB-EL024511HU).ResultsWe found no difference in MG53 levels between the diabetic and control groups (p = 0.914). Furthermore, when the subjects were divided into tertiles according to their MG53 levels, we did not find any difference between the groups in terms of the presence of diabetes (p = 0.981). Additionally, no correlation was observed between weight, BMI, waist circumference, systolic and diastolic blood pressure, fasting blood glucose, HbA1c, albumin excretion in the urine, e-GFR levels, and MG53. Finally, MG53 levels were similar between the groups with and without microvascular and macrovascular complications of diabetes.ConclusionOur research finding provides insightful clinical evidence of lack of association between the levels of MG53 and T2 DM or glycemic control, at least in the studied population of Turkeys ethnicity. However, further clinical studies are warranted to establish solid evidence of the link between MG53, insulin resistance and glycemic control in a wider population elsewhere in the world.Öğe Comparative Investigation of Anti-Inflammatory Effect of Platelet-Rich Fibrin after Mandibular Wisdom Tooth Surgery: A Randomized Controlled Study(Mdpi, 2023) Karaca, Gamze Tanan; Duygu, Gonca; Er, Nilay; Ozgun, ErayThis study evaluated the anti-inflammatory effect of platelet-rich fibrin (PRF) applied to the extraction socket after impacted mandibular third molar surgery with subjective and objective parameters. Forty-eight patients with impacted wisdom teeth in bilateral and similar positions were included in the study. The control group was formed with the standard surgery and the PRF group was formed with local PRF application in addition to standard procedure (n = 96). The anti-inflammatory activity of PRF on postoperative 2nd and 7th days was evaluated subjectively by clinical parameters and objectively by biochemical parameters. Postoperative 2nd- and 7th-day follow-up data of pain, edema, and trismus in the PRF group were found to be statistically significantly lower. Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were found to be statistically significantly lower in the PRF group than the control in the postoperative 2nd-day follow-up period (p < 0.001). There was no statistically significant difference in interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-& alpha;) parameters when the PRF group and the control group were compared in both follow-up periods (p > 0.05). The study has demonstrated the effectiveness of locally applied PRF after ITM surgery via clinical parameters and objective data. The quantitative analysis of CRP and ERS can be an effective parameter in determining the amount of inflammation after ITM surgery.Öğe The effect of L-carnitine on nitric oxide metabolism in streptozotocin-induced diabetic rats(Walter De Gruyter Gmbh, 2014) Ozgun, Gulben Sayilan; Ozgun, Eray; Eskiocak, Sevgi; Sut, NecdetObjective: The aim of this study is to investigate the effect of L-carnitine on plasma and liver nitric oxide metabolism in streptozotocin-induced diabetic rats. Methods: Sprague Dawley female rats were divided randomly into following groups: control, L-carnitine, diabetes and diabetes+L-carnitine. Diabetes and diabetes+L-carnitine groups were intra-peritonally injected with a single dose of streptozotocin (40 mg/kg) prepared in the citrate buffer (pH 4.5). Other groups were injected with only citrate buffer. 72 hours after the streptozotocin injection, L-carnitine (500 mg/kg/day) was given intraperitoneally to L-carnitine and diabetes+L-carnitine groups for 15 days. Physiological saline was given intraperitoneally to the other groups for 15 days. Blood sugar (at 72 hours and the end of experiment), liver nitric oxide and inducible nitric oxide synthase, plasma nitric oxide and nitrotyrosine levels were measured. Results: Blood glucose levels in diabetic groups were higher compared with other groups. Percentage change of blood glucose in diabetes+L-carnitine group was lower compared with other groups. Also diabetes+L-carnitine group's plasma nitric oxide levels were higher than control group. Plasma nitrotyrosine levels of L-carnitine injected groups were lower than diabetes group. There was no significant difference between the levels of liver inducible nitric oxide synthase and nitric oxide in groups. Conclusion: As a result, our study showed that plasma and liver nitric oxide and liver inducible nitric oxide synthase levels aren't changed significantly but plasma nitrotyrosine levels are increased at the end of 15th day of experimental diabetes. On the other hand, our results also showed that L-carnitine causes an increase in plasma nitric oxide levels and a decrease in plasma nitrotyrosine levels whereas it has no effect on liver nitric oxide and inducible nitric oxide synthase levels.Öğe Effect of L-carnitine on serum paraoxonase, arylesterase and lactonase activities and oxidative status in experimental colitis(Walter De Gruyter Gmbh, 2013) Ozgun, Eray; Ozgun, Gulben Sayilan; Eskiocak, Sevgi; Yalcin, Omer; Gokmen, Selma SuerAim: Oxidative stress plays an important role in the pathogenesis of inflammatory bowel disease. We investigated antioxidant L-carnitine effect on activities of paraoxonase 1 enzyme which is also synthesized in colon and oxidative status in experimental colitis. Material and Methods: Wistar albino female rats were divided into four groups randomly: control, colitis, pre-treatment and treatment groups. To induce colitis, single dose of 1 mL acetic acid (%4) was given intrarectally to colitis, pre-treatment and treatment groups. Single dose of 500 mg/kg L-carnitine was given intraperitoneally 1 hour before inducing colitis to pre-treatment group and 24 hours after inducing colitis to treatment group. All groups were sacrificied 48 hours after intrarectally administration. Existence of colitis was confirmed by histopathological changes. Paraoxonase, arylesterase and lactonase activities, total oxidant and antioxidant status, malondialdehyde, and total sialic acid were measured in serum. Oxidative stress index was calculated from the formula. Results: While serum malondialdehyde, total sialic acid, total oxidant status and oxidative stress index were significantly elevated, serum paraoxonase, arylesterase and lactonase activities and total antioxidant status were significantly decreased in acetic-acid induced experimental colitis. In acetic-acid induced experimental colitis, L-carnitine caused a significant decrease in serum malondialdehyde, total sialic acid, total oxidant status and oxidative stress index but a significant increase in serum arylesterase and lactonase activities of treatment group only. Conclusion: L-Carnitine has an increasing effect on serum arylesterase and lactonase activities and decreasing effect on oxidative stress in acetic acid-induced experimental colitis. Therefore, L-carnitine may be useful for the treatment of inflammatory bowel disease.Öğe Effect of lipoic acid on paraoxonase-1 and paraoxonase-3 protein levels, mRNA expression and arylesterase activity in liver hepatoma cells(General Physiol And Biophysics, 2017) Ozgun, Eray; Ozgun, Gulben Sayilan; Tabakcioglu, Kiymet; Gokmen, Selma Suer; Sut, Necdet; Eskiocak, SevgiParaoxonase-1 (PON1) and paraoxonase-3 (PON3) are anti-atherosclerotic enzymes, synthesized primarily in liver and bound to HDL in circulation. The aim of the present study was to investigate the effects of therapeutic doses of lipoic acid on PON1 and PON3 protein levels, mRNA expression and arylesterase activity in liver. We treated HepG2 cells with 10, 40 and 200 mu M lipoic acid for 72 h. Cell viability was evaluated by 3-(4,5-dimethy1-2-thiazoly1)-2,5-dipheny1-2Htetrazolium bromide assay. PON1 and PON3 protein levels were measured by Western blotting, their mRNA expression was measured by quantitative PCR and arylesterase activity was measured spectrophotometrically. 200 mu M lipoic acid caused a significant increase on PON1 and PON3 protein levels and arylesterase activity as compared with control, 10 mu M and 40 mu M lipoic acid treated cells. 200 mu M lipoic acid also caused a significant decrease on PON1 mRNA expression whereas on a significant increase PON3 mRNA expression as compared with control, 10 mu M and 40 mu M lipoic acid-treated cells. Our study showed that although lip oic acid up-regulates PON3 but down-regulates PON1 mRNA expression, it increases both PON1 and PON3 protein levels and arylesterase activity in HepG2 cells. We can report that lipoic acid may be useful for preventing atherosclerosis at therapeutic doses.Öğe Effects of acrylamide on protein degradation pathways in human liver-derived cells and the efficacy of N-acetylcysteine and curcumin(Taylor & Francis Ltd, 2022) Al-Hajm, Abdullah Yahya Salih; Ozgun, ErayAcrylamide is a harmful chemical, and its metabolism occurs mainly in the liver. Acrylamide can form adducts on proteins. Protein homeostasis is vital for metabolic and secretory functions of the liver. No study has investigated the effect of acrylamide on the ubiquitin-proteasome system (UPS). Also, the effect of acrylamide on autophagy and its regulation is not fully known. We aimed to investigate the effects of acrylamide on the UPS, autophagy, mammalian target of rapamycin (mTOR), and heat shock protein 70 (HSP70) in HepG2 cells as well as to examine the effects of N-acetylcysteine and curcumin on these parameters in acrylamide-treated cells. HepG2 cells were initially treated with variable concentrations of acrylamide (0.01-0.1-1-10 mM) for 24 hours. Then, HepG2 cells were treated with 5 mM N-acetylcysteine and 6.79 mu M curcumin in the presence of 10 mM acrylamide for 24 hours. Cell viability was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Ubiquitinated protein, mTOR, microtubule-associated proteins 1 A/1B light chain 3B-II (LC3B-II), and HSP70 levels were measured by immunoblotting. Acrylamide at 10 mM concentration, without any significant change at lower concentrations, caused an increase in ubiquitinated protein, LC3B-II, and HSP70 levels and a decrease in mTOR phosphorylation. Furthermore, 5 mM N-acetylcysteine caused a decrease in ubiquitinated protein and HSP70 levels; however, 6.79 mu M curcumin did not affect 10 mM in acrylamide-treated cells. Our study showed that acrylamide at high concentration inhibits UPS and mTOR, activates autophagy, and increases HSP70 levels in HepG2 cells, and N-acetylcysteine reduces UPS inhibition and HSP70 levels in acrylamide-treated cells.Öğe Falsely Elevated Thyroid Stimulating Hormone in Two Cases Requiring Special Follow-up(Galenos Publ House, 2023) Colak, Serpil Yanik; Ozgun, Eray; Andac, Burak; Okur, Mine; Bulbul, Buket Yilmaz; Celik, MehmetInappropriate thyroid stimulating hormone (TSH) refers to an elevation in TSH levels that does not match the clinical findings and free T3 and free T4 levels. Several conditions can cause this, such as pituitary tumors that produce TSH, resistance to thyroid hormones, macro-TSH, and antibody interference. Macro-TSH is a condition that causes TSH to be measured high in the blood for a long time by forming a complex with immunoglobulins, mostly IgG. However, patients are clinically euthyroid because macro-TSH is not a bioactive complex. It is essential to exclude the diagnosis of falsely elevated TSH to protect patients from unnecessary or high -dose levothyroxine therapy. In our first case, we presented a patient in whom subclinical hypothyroidism was detected during in vitro fertilization treatment, and levothyroxine was started. The other case was an operated papillary thyroid cancer patient. In both cases, although the dose of levothyroxine was increased, insufficient TSH response to increased fT4/fT3 levels suggested inappropriate TSH elevation. The polyethylene glycol (PEG) precipitation method was used to detect the assay variability. TSH recovery after PEG was 0.96016 and 21016, respectively, supporting the diagnosis of macro-TSH. In both cases, detecting Macro-TSH was crucial in preventing thyrotoxicosis caused by excessive levothyroxine dosage. In addition, delay in treatment for infertility was prevented in the first case.Öğe Increased fibrinogen to albumin ratio in ischemic retinal vein occlusions(Sage Publications Ltd, 2017) Guclu, Hande; Ozal, Sadik A.; Gurlu, Vuslat Pelitli; Ozgun, Gulben Sayilan; Ozgun, ErayPurpose: To demonstrate the relationship between ischemia and plasma fibrinogen and serum albumin levels in cases of retinal vein occlusion (RVO). Methods: This study included 44 patients with central RVO (CRVO), 68 patients with branch RVO (BRVO), and 54 age- and sex-matched controls, for a total of 166 subjects. All of the subjects underwent full ophthalmologic examinations and complete physical examinations, including a detailed medical history and blood count, and biochemical parameters. Results: The mean fibrinogen to albumin ratios were 92.5 +/- 36.1 for the patients with CRVO, 84.5 +/- 31.5 for the patients with BRVO, and 68.4 +/- 12.2 for the control group. Overall, the patients with CRVO and patients with BRVO with ischemia had higher fibrinogen to albumin ratios and higher fibrinogen levels. Moreover, significant positive correlations were found between ischemia and the fibrinogen to albumin ratio (r = 0.732, p = 0.001) and the fibrinogen level (r = 0.669, p = 0.001). Conclusions: The fibrinogen to albumin ratio is significantly associated with ischemic RVO. Instead of complicated and invasive methods, such as a retinal angiogram, the fibrinogen to albumin ratio could be a useful initial diagnostic test to predict ischemia in RVO.Öğe Investigation of the effects of ellagic, vanillic and rosmarinic acid on reperfusion-induced renal injury(Clinics Cardive Publ Pty Ltd, 2023) Gurmen, Alper; Guclu, Orkut; Huseyin, Serhat; Can, Nuray; Ozgun, Eray; Buyukadali, Mursel; Reyhancan, AdemIntroduction: The aim of this study was to investigate the effects of ellagic, vanillic and rosmarinic acid on reperfusion-related kidney damage, developed in an experimental lower-extremity ischaemia/reperfusion (I/R) model.Methods: Forty-eight female Sprague-Dawley rats were divid-ed into six groups. A median laparotomy and dissection were performed. In the I/R group, 60 minutes of ischaemia followed by 120 minutes of reperfusion was achieved. In addi-tion one group was given 100 mg/kg ellagic acid, one group was given 12 mg/kg vanillic acid, one group was given 50 mg/ kg rosmarinic acid and one group was given all three drugs 15 minutes before clamp removal. Bilateral kidney and blood samples were taken in all groups.Results: Tubular epithelial degeneration, necrosis of the tubule epithelium and vessel wall thickening were significantly higher in the I/R group. Some parameters in the groups that were given drugs were found to be lower than in the I/R group and close to that of the control group. Total oxidant status (TOS) and oxidative stress index (OSI) were significantly higher and total antioxidant status (TAS) was significantly lower in the I/R group. Although not statistically significant in the groups given drugs, TAS was higher, and TOS and OSI were lower than in the I/R group.Conclusion: The antioxidant effect of ellagic, vanillic and rosmarinic acid administration may have beneficial effects on renal damage after reperfusion in acute lower-extremity ischaemia. This study is expected to provide information for future clinical trials.Öğe Pericardial SCUBE1 levels may help predict postoperative results in patients operated on for coronary artery bypass graft surgery(Clinics Cardive Publ Pty Ltd, 2021) Ozkara, Taha; Yuksel, Volkan; Guclu, Orkut; Huseyin, Serhat; Ozgun, Eray; Turan, Fatma Nesrin; Canbaz, SuatIntroduction: Signal peptide-CUB epidermal growth factor like domain-containing protein (SCUBE1) is a newly described, secretable and measurable cellular surface protein associated with atherosclerotic lesions in humans, which may be involved in hypertension and cardiovascular pathologies. We aimed to detect normal SCUBE1 levels in pericardial fluid and investigate the effects of SCUBE1 values on postoperative outcomes after coronary artery bypass surgery. Methods: Between February 2016 and March 2017, 184 consecutive patients were included in the study. Group 1 consisted of patients with unstable angina pectoris, group 2 of patients with non-ST-elevation myocardial infarction, group 3 of patients with ST-elevation myocardial infarction, and group 4 consisted of patients operated on due to non -coronary reasons. Pericardial fluid and arterial blood SCUBE1 values, demographic variables and postoperative results were noted and compared. Results: Normal SCUBE1 level in pericardial fluid was 0.049 +/- 0.061 ng/ml. Arterial SCUBE1 levels of smokers were higher. Pericardial SCUBE1 levels were higher in patients requiring postoperative intra-aortic balloon pump support and patients needing peri-operative temporary cardiac pacing. High pericardial SCUBE1 values did not correlate with postoperative stroke, prolonged intensive care unit stay and mortality. Conclusion: High levels of pericardial SCUBE1 may help us predict the need for postoperative intra-aortic balloon pump support and the need for temporary cardiac pacing, however they were not helpful in predicting prolonged intensive care unit stay and early postoperative mortality.Öğe Protective effects of ?-lipoic acid and L-carnitine in liver ischemia/reperfusion injury(Turkish Biochem Soc, 2014) Ozgun, Gulben Sayilan; Ozgun, Eray; Basaran, Usmit Nusret; Altaner, Semsi; Sut, Necdet; Eskiocak, SevgiObjective: Oxidative stress plays an important role in the pathogenesis of liver ischemia/reperfusion injury. Thus, antioxidant treatment can be protective against to liver ischemia/reperfusion injury. The aim of this study to investigate the effects of alpha-lipoic acid and L-carnitine on liver total oxidant status, lipid peroxidation, protein oxidation, neutrophil infiltration and hepatic necrosis in liver ischemia/reperfusion model. Methods: Wistar albino male rats were divided into four groups randomly: Sham (n=7), ischemia/reperfusion (n=7), alpha-lipoic acid (n=8) and L-carnitine (n=8). alpha-Lipoic acid (100 mg/kg) and L-carnitine (100 mg/kg) were given intraperitoneally to alpha-lipoic acid group 15 minutes before and to L-carnitine group 30 minutes before ischemia/reperfusion protocol, respectively. To induce hepatic ischemia/reperfusion injury, ischemia (60 minutes) and reperfusion (30 minutes) were applied to all groups except sham group. Total oxidant status, malondialdehyde, advanced oxidation protein products and myeloperoxidase levels were measured in ischemic lobes of liver tissues. Hepatic necrosis was scored microscopically. Results: There was no significant change in myeloperoxidase levels as an indicator of neutrophil infiltration after reperfusion procedure. Both L-carnitine and alpha-lipoic acid caused a significant decrease in hepatic necrosis. While L-carnitine prevents an increase in total oxidant status, lipid peroxidation and protein oxidation, alpha-lipoic acid prevents only an increase in lipid peroxidation of the liver in hepatic ischemia/reperfusion injury. Conclusion: As a result; we can report that L-carnitine and alpha-lipoic acid have protective effects against to hepatic ischemia/reperfusion injury.
