Yazar "Ozdemir, Kezban" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    Genotoxic effects of diazinon on human peripheral blood lymphocytes
    (Inst Medical Research & Occupational Health, 2015) Muranli, Fulya Dilek Gokalp; Kanev, Martin; Ozdemir, Kezban
    The aim of this study was to evaluate the genetic damage in human peripheral blood lymphocytes following 24 and 48-hour exposure to a commercial diazinon formulation Basudin 60EM (R) at concentrations between 0.01 and 40 mu g mL(-1). For this purpose we used the micronucleus (MN), fluorescence in situ hybridization (FISH), and alkaline single cell gel electrophoresis (comet) assay. Diazinon significantly increased the frequency of micronucleated cells compared to control. Forty-eight-hour exposure increased this frequency even at lower concentrations (0.01-10 mu g mL(-1)). The FISH results revealed aneugenic effects at 10 mu g mL(-1). The comet assay also confirmed DNA damage at concentrations between 10 and 40 mu g mL(-1). Our findings have confirmed the genotoxic potential of diazinon and its cytotoxic effect on human lymphocytes. The increased DNA damage in our study raises concern about the current assessment of the health risk posed by this pesticide and calls for a high level of caution in agricultural and household use.
  • Küçük Resim Yok
    Öğe
    POTENTIAL GENOTOXICITY OF ACETAMIPRID AND PROPINEB SINGLY OR IN COMBINATION IN CULTURED HUMAN PERIPHERAL BLOOD LYMPHOCYTES BY USING MN ASSAY
    (Parlar Scientific Publications (P S P), 2015) Muranli, Fulya Dilek Gokalp; Rasgele, Pinar Goc; Kekecoglu, Meral; Kanev, Martin; Ozdemir, Kezban
    Acetamiprid, a neonicotinoid insecticide, and Propineb, a dithicarbamate fungicide, are used in agriculture singly or in combination with other pesticides. The aim of the study is to investigate the single and combined genotoxic effect two pesticides on cultured human lymphocytes by using the Micronucleus (MN) assay. The lymphocytes were treated with three different concentrations of pesticides singly and in combination (a mix), Acetamiprid (0.625, 1.25, 2.5 mu g mL(-1)) Propineb (12.5, 25, 50 mu g mL(-1)) and Acetamiprid+Propineb (0.625+12.5, 1.25+25, 2.5+50 mu g mL(-1)), for 24 and 48 hours. MN frequency significantly increased at a 48-hour exposure period when the lymphocytes were exposed to the combined pesticides. The data demonstrated that these pesticides may have a synergistic effect and may have potential genotoxic activity in cultured human lymphocytes. Also, it was shown that when they are combined and exposed to lymphocytes these pesticides have a cytotoxic effect.