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    Diversity analysis of the rice cultivars (Oryza sativa L.) by utilizing SSRs rice diversity by SSRs
    (Wiley, 2021) Beser, Necmi; Mutafcilar, Zeynep Cisem; Hasancebi, Semra
    This study was carried out to analysis of genetic diversity of rice marketed in Turkey. Sixty rice varieties used as a material. A total of 50 SSR markers distributed to all 12 rice chromosomes were used for the diversity analysis of the genotypes. Out of these, 43 SSRs produced clear, distinct polymorphic bands among the rice varieties, and a total of 227 alleles were obtained. The number of alleles per locus ranged from 9 to 1. The mean number of alleles per locus was 5.25, and 22 markers (51%) had 6 or more alleles. Clustering of 60 varieties based on 43 SSR markers was carried out. While aromatic varieties were placed into an out-group Rocca and Baldo which are parents of many rice varieties and 47 varieties were placed into the same clusters. This SSR polymorphism analysis revealed genomic relationships in rice marketed in Turkey. Practical applications Results of this study could be used to diversity analysis of rice and other crops and rice breeding programs. It could be used to study genetic diversity of polished rice at the market and consumer preference by countries.
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    Identification of SSR Markers for Differentiating Rice (Oryza sativa L.) Varieties Marketed in Turkey
    (Galenos Publ House, 2020) Beser, Necmi; Mutafcilar, Zeynep Cisem
    This study was carried out to identify SSR markers for the differentiation and identification of rice cultivars marketed in Turkey between 2016 and 2017. In this study 60 registered or production permitted, some local and foreign rice varieties were used as a material. DNA was isolated from single polished rice kernels for PCR amplification and rice cultivars were genotyped by 50 SSR markers. We found that 45 out of 50 SSR markers produced reproducible and polymorphic alleles. Thirty-six rice varieties had variety specific alleles among 60 rice varieties analysed in the study. This variety specific alleles belong to only one variety and they can be used to identify rice variety among 60 rice varieties studied. Osmancik-97, Cammeo, Ronaldo and Baldo are the most import 4 varieties in Turkish rice production and milled rice market. In this study, it was found that RM152, RM144, RM259 and RM118 SSR markers can be used to differentiate and identify these most important four varieties with different combinations. Collectively, this study provided some variety specific SSR markers that can be used to differentiate and identify rice varieties sold in Turkey.