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Öğe Comparison of two different enzyme immunoassays in the diagnosis of fasciola hepatica infections(2004) Şakru N.; Korkmaz M.; Kuman H.A.In this study, the serum samples of 37 patients who were diagnosed as fascioliasis (=fasciolosis) by parasitologic, pathologic and/or radiographic methods, 51 patients who were previously infected with parasites other than Fasciola hepatica, and 31 healthy subjects, have been investigated for the presence of F.hepatica specific lgG antibodies with two different enzyme immunoassay (ELISA) methods. For this purpose, in-house DOT-ELISA and excretory-secretory (ES)-ELISA techniques have been performed by using F.hepatica ES antigens. All of the 37 fasciolosis patients found to be seropositive with ES-ELISA, while 36 were found positive with DOT-ELISA method. ES-ELISA method yielded 3 (5.9%) positive results for the patients with other parasitosis (2 toxocariasis, 1 hydatic cyst), and DOT-ELISA method gave positive results for 2 (3.9%) toxocariasis patients. In 31 healthy subjects, the seropositivity rates were detected as 3.2% and 16.1% for ES-ELISA and DOT-ELISA methods, respectively. As a result, the sensitivity and specificity of DOT-ELISA method were found as 97.3% and 93.3%, respectively, while these rates were 100% and 97.0% for ES-ELISA, respectively. The highest positive predictive value was detected for ES-ELISA (90.2%), while both of the methods showed high negative predictive values (100% for ES-ELISA, 99.1% for DOT-ELISA). In conclusion, ELISA methods would be useful especially for the laboratory diagnosis of fasciolosis, in the clinically suspected patients where the etiologic agent could not be identified.Öğe The factors affecting the organizational silence of the nurses and the other clinical-care providers in Turkey(American-Eurasian Network for Scientific Information, 2014) Toker F.; Korkmaz M.; Kılıç B.; Yücel A.S.; Gümüş S.The organizational silence of nurses, midwives, and health-care assistants who play a key role to establish the communication in the health-care team is relatively more significant when compared to other occupations. The results of organizational silence can be examined in organizational, individual and social scale. The purpose of this study is to find out the reasons of organizational silence and the relations among these reasons. In the scope of analysis, descriptive statistcs, reliability analysis, correlation analysis, ANOVA analysis and Regression analyses have been used by using PASW 18.0 package software to analyze the data gathered from 256 nurses, midwives and health-care assistants in a university hospital. In this study it has been found out that the organizational silence reasons differ according to age, education status, working year, occupation, working department and gender. It has also been determined that the administrative and organizational reasons, the fears related to work, the lack of experience, the fear of isolation and the fear of disturbing relations differ according to demographical variables. Since there is generally not a compensantion for a mistake in health-care service, to remove the damages of the reasons of organizational silence in health-care sector which is relatively more significant than the other sectors is possible when the necessary precautions are taken and the reasons are identified correctly. The relations, similarities and the differences among the sectors can be presented, if the organizational silence studies both in health-care sector and in different sectors are carried out more. © 2014 AENSI Publisher All rights reserved.Öğe Factors affecting the perceived hospital quality(American-Eurasian Network for Scientific Information, 2014) Toker F.; Korkmaz M.; Yücel A.S.; Kılıç B.Quality perception, which is related to a number of factors and variables, has come to the fore both because of the features of health services and because of the hospital quality rating practices of the Ministry of Health in Turkey. Although there are national and international studies to assess the perceived quality, with the thought that countryspecific studies are not enough, this study has been thought necessary in order to learn the main factors which affect public's hospital quality perception in Turkey due to the facts that culture is an important determinant on quality perception and the results of the year-to-date studies are different from each other. In Turkey's 3 biggest cities, 2500 people have been asked 28 questions with demographical and descriptive features in the questionaire in order to define hospital quality and these questions have been perceived in 4 dimensions. Non-parametric and parametric statistical tests have been applied on the data gathered. In the scope of analysis, descriptive statistics, reliability analysis, ANOVA analysis, and Regression analyses have been used. According to the results of this study, the idea of a high quality hospital changes according to age, income, education and the city; the tendency to the idea of a well managed hospital changes according to gender, income, education, and the city and the idea of low-quality changes according to age, gender, income, education and the city. Whereas education is the most effective variable in high-quality hospital aspect, gender is the most effective variable on low-quality hospital. In this study, it has been determined that hospital quality perception changes according to the demographical features as age, income, education and the city resided. This study has been reinforced with the fact that there has been a significant difference in terms of age groups and level of education in Devebakan and Aksarayli's study in a private hospital with SERVQUAL scale. We are made to think that these researches has to be carried out more owing to the fact that there has been similar and different results with different scales in different organizations. © 2014 AENSI Publisher All rights reserved.Öğe Fasciola hepatica infection in echinococcosis suspected cases.(2011) Sakru N.; Korkmaz M.; Demirci M.; Kuman A.; Ok U.Z.Fasciola hepatica, a liver fluke of sheep and cattle, may accidentally infect humans. The main signs and symptoms of the fasciolosis are eosinophilia, abdominal pain and hepatomegaly and may also be attributed to Cystic Echinococcosis (CE) caused by Echinococcus granulosus, which is a prevalent infection in Turkey. Sera samples of 226 CE suspected cases were tested for anti-F. hepatica antibodies by an excretory secretory ELISA (ES-ELISA) and for anti-E. granulosus antibodies by ELISA and indirect hemagglutination (IHA) tests. Cases which were seropositive for fasciolosis were further evaluated radiologically and examined for F. hepatica eggs. Five (2.2%) and 96 (42.4%) of the 226 CE suspected cases were found seropositive for fasciolosis and CE, respectively. Although the radiological findings strongly suggested that there was fasciolosis in three cases, F. hepatica eggs were detected in two patients only. These data suggest that human fasciolosis is not as rare as previously reported in Turkey. F. hepatica infection should be suspected especially in the presence of eosinophilia, abdominal pain and liver lesions.Öğe Serological monitoring of paediatric visceral leishmaniasis By IFA and ELISA methods.(2011) Şakru N.; Töz S.O.; Korkmaz M.; Özbel Y.Visceral leishmaniasis (VL) is endemic in all Mediterranean countries including Turkey, and children are at greater risk than adults in endemic areas. In VL patients, serological assays are considered to be sensitive for the diagnosis and/or follow up. The aim of this study was to assess the usefulness of serology for following up of childhood VL in Turkey. Sera obtained from twenty parasitologically confirmed children with VL were tested using IFAT and ELISA. The patients were monitored clinically and serologically (range: 20-500 days) during and after treatment. All VL patients were treated with meglumine antimonate. Anti-Leishmania antibodies in successfully treated VL patients showed a steep decline but, in three patients who had relapsed, an increase was detected. Significantly lower values were observed after treatment with both serological techniques. Mean ELISA optical density values before and after treatment were: 0.78 ± 0.36 (0.26-1.76) and 0.38 ± 0.24 (0.09-0.83) respectively, (p < 0.001) and mean IFAT values (log10 transformed titers) before and after treatment were: 3.02 ± 0.90 (1.81-4.51) and 2.16 ± 0.75 (1.20-3.90) respectively, (p < 0.001). ELISA and IFAT are valuable not only for diagnosis but also for monitoring of drug therapy in childhood visceral leishmaniasis as rapid and non-invasive techniques.
