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Öğe The alteration of asymmetric dimetilarginine (ADMA) levels in cardiac and gastrocnemius muscles following radioactive iodine application in guinea pigs and the effect of L-carnitine on this alteration(Taylor & Francis Ltd, 2011) Vardar, Selma Arzu; Gunduz, Ozgur; Altun, Gulay Durmus; Aydogdu, Nurettin; Karadag, Hakan; Torun, Nese; Kaya, OktayPurpose: Tissue levels of asymmetric dimetilarginine (ADMA) and symmetric dimetilarginine (SDMA) were investigated in cardiac ventricle and gastrocnemius muscles of guinea pigs treated with radioactive iodine (RAI) alone or in combination with L-carnitine (LC). Material and methods: Group 1 received no treatment (control group). Group 2 received a total dose of 30 mCi(-1)kg(-1) body weight iodine-131 alone. Group 3 received 200 mg(-1)kg(-1) of LC for 10 days alone. Group 4 received 200 mg(-1)kg(-1) of LC plus RAI therapy. Free thyroid hormones, ADMA and SDMA concentrations were measured. Results: Serum free thyroid hormone concentrations were found decreased in the RAI and LC-RAI groups after RAI application. A significant decrease in ADMA and SDMA concentration was observed in ventricle muscle following RAI application. The LC-RAI group had significantly decreased ADMA levels in ventricle muscle compared with those of the control group. Similarly, SDMA concentrations in ventricle and gastrocnemius muscles of the LC-RAI groups were significantly lower than those of the control groups. Conclusions: Our results indicated that RAI appears to exert an inhibitory effect on ADMA and SDMA levels of ventricular muscle. LC administration when given adjuvant to RAI therapy may cause a marked decrease in ADMA concentrations of both ventricular and gastrocnemius muscles.Öğe Ameliorative Effect of Omega-3 in Carbon Tetrachloride Toxicity(Kafkas Univ, Veteriner Fakultesi Dergisi, 2017) Karaman, Musa; Ozen, Hasan; Dag, Serpil; Atakisi, Onur; Cigsar, Gulsen; Kaya, OktayOmega-3 is a polyunsaturated fatty acid known to have immunomodulatory functions. In the present study, ameliorative potential of omega-3 in experimental carbon tetrachloride (CCl4) toxicity was investigated. Total of 40 adult male Wistar albino rats were allocated into five groups and were subcutaneously given once every two days for 6 weeks the followings: Group 1 (Control): 0.5 ml/kg serum physiologic, Group 2 (Omega): 0.5 g/kg omega-3, Group 3 (Vehicle): 0.5 ml/kg pure olive oil, Group 4 (CCl4): 0.5 ml/kg CCl4, Group 5 (CCl4 + Omega): 0.5 ml/kg CCl4 plus 0.5 g/kg omega-3. At the end of the treatments, blood samples were collected and necropsy was performed for collection of liver tissues. Serum AST, ALT, GGT, TAC, TOC, triglyceride, and visfatin levels were detected. Liver morphology and immunoreactivities against TGF-alpha, TGF-beta, PPAR-alpha, and PPAR-gamma were assessed. Serum AST, ALT, GGT, and TOC levels significantly increased while TAC level decreased in CCl4 given animals as compared to the control group. No significant changes were observed in triglyceride and visfatin levels. Immunohistochemical staining revealed increased TGF-alpha and TGF-beta expressions and decreased PPAR-alpha and PPAR-gamma expressions in liver of CCl4 given animals. Omega-3 supplementation has prominent effects in correcting the biochemical and immunohistochemical parameters studied as well as the tissue morphology. The results of the investigation indicated that omega-3 has ameliorative effects on the oxidative tissue degeneration and inflammatory processes induced by CCl4 treatment in rats.Öğe Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis(Mdpi, 2023) Gunduz, Ozgur; Sapmaz-Metin, Melike; Topuz, Ruhan Deniz; Kaya, Oktay; Karadag, Cetin Hakan; Ulugol, AhmetBackground and Objectives: Allergic contact dermatitis is a common type IV hypersensitivity reaction characterised by redness, itching, oedema and thickening of the skin. It occurs in about 7% of the population and its incidence is increasing. It has been observed that the preconditioning of tissues by exposing them to transient ischemia increases resistance to subsequent permanent ischemia, and this phenomenon is called ischemic preconditioning. It has been shown that conditioning in one organ can also protect other organs. The protective effect of remote ischemic preconditioning is thought to be based on the induction of anti-inflammatory responses. The aim of this project was to investigate the anti-inflammatory and antipruritic effects of remote ischemic postconditioning in a mouse model of experimental allergic contact dermatitis. Methods: Experimental allergic contact dermatitis was induced with 1-fluoro-2,4-dinitrobenzene. Remote ischemic postconditioning was performed at 3 and 25 h after the challenge. Ear thickness and number of scratches 24 and 48 h after challenge, as well as cytokine levels and the infiltration of mast cells, neutrophils, CD4(+) and CD8(+ )T lymphocytes in serum and ear tissue at 48 h were measured to determine the effect of RIPsC. Results: Remote ischemic postconditioning decreased ear thickness, one of the symptoms of allergic contact dermatitis (p < 0.0001). It had no significant effect on the number of scratches. It reduced serum IL-17 levels (p < 0.01). It alleviated local inflammation by suppressing CD8+ T lymphocyte and neutrophil infiltration. Conclusions: It was concluded that remote ischemic postconditioning may alleviate the symptoms of allergic contact dermatitis by suppressing CD8(+ )T lymphocyte and neutrophil infiltration and reducing IL-17 secretion.Öğe Blood pressure, autonomic stress, and inflammatory markers during sleep deprivation and recovery in healthy men(Turkish Soc Cardiology, 2021) Bozer, Ozge; Kaya, Oktay; Ozturk, Gulnur; Bulut, Erdogan; Zorkun, Cafer; Ozturk, LeventObjective: Recent community-based studies have identified sleep deprivation (SD) as an important modifiable risk factor for hypertension However, the underlying mechanisms linking SD to hypertension remain elusive. Thus, this study investigates blood pressure (BP) responses to cardiac autonomic stress tests in the presence of SD. Furthermore, we analyzed vascular inflammatory biomarkers as a possible underlying factor linking SD to increased BP. Methods: Ten healthy male volunteers (age, 21.6 +/- 1.2 years) underwent repeated autonomic stress tests for three consecutive days (baseline, SD, and recovery). The autonomic stress tests included the Valsalva maneuver, mental arithmetic, isometric handgrip, and cold pressor tests. Each day, resting BPs were measured, venous blood samples were collected for intercellular adhesion molecule 1, vascular cell adhesion molecule 1, and E-selectin measurements, and stress tests were performed between 0900 and 1100. Ambulatory BP was recorded during the entire SD period (24 h). Results: One-night SD abolished BP reactivity to the Valsalva maneuver, isometric hand grip, and cold pressor tests, which returned after recovery sleep. Ambulatory BP monitoring showed that the mean systolic and diastolic BPs were 121.1 +/- 8.5 mm Hg and 72.8 +/- 6.3 mm Hg, respectively, between 0700 and 2300 and 120.3 +/- 9.6 mm Hg and 74.1 +/- 6.1 mm Hg, respectively, between 2300 and 0700 during the SD day (p>0.05 for both). Vascular inflammatory markers seemed unrelated to BP changes. Conclusion: Acute SD altered BP responses to cardiac autonomic stress tests in healthy men without affecting resting BP levels. SD led to a non-dipping pattern in BP oscillation. Collectively, these findings highlight the importance of sleep in regulating BP.Öğe Circadian/Homeostatic Regulation of Body Temperature and Sleep(Wiley, 2019) Kaya, Oktay[Abstract Not Available]Öğe The Comparison of the Perceived Exertion of Physically Active and Non-Active Men During High Intensity Cycling Intervals(Wiley, 2018) Vardar, Selma A.; Doganlar, Zeynep B.; Kaya, Oktay; Tayfur, Pinar; Sut, Necdet; Merakli, Meryem A.; Doganlar, Oguzhan[Abstract Not Available]Öğe Deneysel miyoglobinürik akut böbrek hasarında kannabinoid reseptör agonist ve antagonistlerinin etkileri(Sağlık Bilimleri, 2021) Erge, Ümmühan; Kaya, OktayEndokannabinoid sistemin böbrek fizyolojisi ve patofizyolojisinde önemli rol oynadığı bildirilmiştir. Çalışmamızda kannabinoid reseptörü agonist ve antagonistlerinin miyoglobinürik akut böbrek hasarında biyokimyasal ve histopatolojik parametreler üzerine etkilerini ve bu etkilere oksidatif stresin olası katkısını incelemeyi amaçladık. Çalışmamızda 7 grupta toplam 56 adet Wistar dişi sıçan kullanıldı. Kontrol grubuna serum fizyolojik diğer gruplara %50’lik gliserolün intramüsküler enjeksiyonları ile model oluşturuldu. Gruplara 60. ve 75. dakikalarda intraperitoneal enjeksiyonlar yapıldı [sırasıyla (kontrol: sf,sf), (ABH: sf,sf), (AM251: AM251,sf), (SR 144528: SR144528,sf), (WIN+AM251: WIN,AM251), (WIN+SR144528: WIN, SR144528)]. İntramüsküler enjeksiyonlardan sonra metabolik kafese alınan sıçanların 24 saatlik idrarları toplandı. Ardından anestezi altında kan ve böbrekleri alındı. Böbrek dokusunda glutatyon, malondialdehit düzeyleri, histopatolojik değişiklikler; serumda üre, kreatinin, sodyum düzeyleri; idrarda kreatinin, sodyum düzeyleri incelendi. Çalışmamızda gliserol uygulanan grupların tamamında kontrol grubuna göre serum kreatinin ve üre seviyelerinde anlamlı bir artış gözlenirken idrar sodyum seviyelerinde anlamlı bir azalma gözlendi. WIN+SR144528 grubunda ABH grubuna göre serum kreatinin düzeyinin anlamlı olarak daha düşük olduğu görüldü. SR144528 grubunda WIN grubuna göre serum üre düzeyi daha düşüktü. MDA düzeyi WIN+AM251 grubunda SR144528 grubuna göre anlamlı olarak daha yüksekti. Histolojik olarak kontrol grubuyla karşılaştırıldığında gliserol verilen grupların böbrek kesitlerinde değişik şiddette tübüler nekroz ve kast birikimi ile kortikal peritübüler ödem gözlendi. Hasar skoru WIN ve WIN+SR144528 gruplarında ABH grubuna göre anlamlı olarak düşüktü. 48 Tüm bu sonuçlar topluca değerlendirildiğinde eksojen olarak kannabinoid sistemi etkileyecek ajanların uygulanmasının böbrek fonksiyon ve histolojisi üzerinde önemli etkiler ortaya çıkardığı görüldü. Böbrek fonksiyon ve histolojisi üzerine olan bu etkilerde CB2 reseptörlerinin blokajının daha önceki çalışmaların aksine olumlu sonuçları olduğu, CB1 reseptörlerinin blokajının ise olumsuz sonuçlara yol açtığı görüldü. Bu etkilerin ortaya çıkmasında oksidatif stresin önemli bir faktör olarak göz önünde bulundurulması gerektiği düşünüldü.Öğe Deneysel miyoglobinürik akut böbrek yetmezliğinde losartanın etkileri(Trakya Üniversitesi, 2010) Kaya, Oktay; Aydoğdu, NurettinMiyoglobinürik akut böbrek yetmezliği iskelet kaslarının hasarlanmasıyla oluşan üremik bir sendromdur. Serbest radikaller ve nitrik oksidin miyoglobinürik akut böbrek yetmezliği patogenezinde önemli rol oynadığı gösterilmiştir. Losartanın antioksidan etki gösterdiği, nitrik oksit düzeyini artırdığı bildirilmiştir. Çalışmamızda, losartanın deneysel miyoglobinürik akut böbrek yetmezliğindeki etkilerini araştırmayı amaçladık.Çalışmamızda; 370-450 gram ağırlığında 34 adet Spraque-Dawley erkek sıçan kullanıldı. Sıçanlar enjeksiyondan 24 saat önce susuz bırakıldı. 1. ve 2. gruba fizyolojik serum, 3. ve 4. gruba 10 ml/kg dozunda % 50'lik gliserol solüsyonu intramüsküler enjekte edildi. 1 ve 24 saat sonra 1. ve 3. gruba distile su, 2. ve 4. gruba 10 mg/kg losartan oral yolla verildi. Gliserol enjeksiyonundan 48 saat sonra idrarları toplandı ve anestezi altında kan ve böbrekleri alındı. Böbrek dokusunda nitrik oksit, arginin, asimetrik dimetilarginin, simetrik dimetilarginin, glutatyon, malondialdehit düzeyleri, histopatolojik değişiklikler; serumda üre, kreatinin, sodyum, potasyum, arginin, asimetrik dimetilarginin, simetrik dimetilarginin düzeyleri; idrarda nitrik oksit, kreatinin, sodyum düzeyleri incelendi.Çalışmamızda 1. grup ile karşılaştırıldığında 3. grupta serumda üre, kreatinin, potasyum, simetrik dimetilarginin ile böbrek dokusunda malondialdehit ve asimetrik dimetilarginin düzeylerinde artma; serum sodyum, arginin ile böbrek dokusunda simetrik dimetilarginin ve arginin düzeylerinde istatistiksel olarak anlamlı azalma gözlendi. 3. grup ile karşılaştırıldığında 4. Grupta serum kreatinin ve böbrek dokusunda simetrik dimetilarginin düzeylerinde artış, böbrek dokusunda asimetrik dimetilarginin düzeylerinde istatistiksel olarakanlamlı azalma tespit edildi. Diğer bulgular arasında istatistiksel olarak anlamlı bir farklılık görülmedi. 3. grupta 7, 4. grupta 6 sıçan öldü.Bu sonuçlar göz önüne alındığında losartanın miyoglobinürik akut böbrek yetmezliği patogenezi üzerinde olumlu etkilerinin olmadığını düşünüyoruz.Anahtar Kelimeler: Losartan, Miyoglobinürik Akut Böbrek Yetmezliği, Serbest Radikaller, Nitrik Oksit, Asimetrik DimetilargininÖğe The Effect of Exogenous Melatonin on Blood Glucose in A Model of Experimental Chronic Sleep Deprivation(Wiley, 2023) Gul, Tugba Kizil; Kaya, Oktay; Puyan, Fulya Oz; Ozturk, Levent[Abstract Not Available]Öğe The Effect of Music on Subjective Time Perception(Wiley, 2019) Gul, Tugba Kizil; Ozturk, Gulnur; Kaya, Oktay; Sen, Burcu; Memi, Gulsun; Ozturk, Levent[Abstract Not Available]Öğe The effect of platelet-rich plasma in inactive form on the burn zone of stasis in rats(Taylor & Francis Ltd, 2019) Orhan, Erkan; Sapmaz-Metin, Melike; Tarladacalisir-Topcu, Yeter; Gunduz, Ozgur; Kaya, OktayThe protection of the burn stasis zone tissues (BSZT) reduces the width and depth of the burn injury. In this study, it is aimed to show the effect of platelet-rich plasma (PRP) on the burn zone of stasis. Seventy-two Wistar rats were used in the study. PRP was obtained from the blood taken from eight rats. The remaining 64 rats were divided into four groups. In Group 1, only the burn procedure was performed. In Group 2, 0.3 cc of physiological saline solution, in Group 3, 0.3 cc of platelet-poor plasma and in Group 4, 0.3 cc of PRP were intradermally injected into BSZT after burn procedure. 21.5% of the tissues in Group 1, 20.8% in Group 2, 27.0% in Group 3, and 69.6% in Group 4 were found to be alive. The autophagic cell number average was calculated as 340 in Group 1, 340 in Group 2, 335 in Group 3 and 450 in Group 4, while the average number of cells stained with Nrf2 was calculated as 225 in Group 1, 245 in Group 2, 250 in Group 3 and 370 in Group 4. When the groups were compared in terms of the living tissue ratio, autophagy and number of cells stained with Nrf2, the values in Group 4 were found to be statistically significantly higher compared to Group 1, Group 2 and Group 3, while there was no difference between Groups 1, 2 and 3. This study has shown that PRP has a protective effect on BSZT.Öğe Effects of Cannabinoid Modulation on Hypothalamic Nesfatin-1 and Insulin Resistance(Wolters Kluwer Medknow Publications, 2019) Kaya, Oktay; Yilmaz, Makbule Elif; Bayram, Sinasi; Gunduz, Ozgur; Kizilay, Gulnur; Ozturk, LeventBoth nesfatin-1 and cannabinoid systems involved in the regulation of sleep, metabolism, and food intake. The relationship between cannabinoid system and nesfatin-1 levels remains to be elucidated. This study investigated nesfatin-1 and insulin resistance in 72-h rapid eye movement (REM) sleep-deprived mice under the effects of cannabinoid, and cannabinoid receptors CB1R and CB2R blocking. Sixty mice were exposed to 72-h sleep deprivation. Groups and drug administrations were as follows: Group 1 (control) received injection of vehicle. Group 2 received WIN 55,212,2. Group 3 received AM251 (CB1R antagonist) followed by WIN 55,212,2 injection. Group 4 received SR144528 (CB2R antagonist) followed by WIN 55,212,2 injection. Group 5 received only AM251. Group 6 received only SR144528. Blood samples were collected 1 h after drug administration and prepared for biochemical measurements. Glucose levels were measured by glucometer, whereas insulin and nesfatin-1 levels were measured by ELISA. Central nesfatin-1 was also assessed using immunohistochemistry. One-way analysis of variance together with post hoc Tukey's test was used for inter-group comparisons. Serum nesfatin-1 levels were comparable in all study groups. Brain nesfatin-1 immune-positive cell count was lower in WIN group compared to controls. The administration of CB1R or CB2R antagonist prevented reduction in nesfatin-1-positive cell count. Insulin resistance was higher in WINCB2 and CB2 groups than in control and WINCB1 groups. Cannabinoid treatment reduced nesfatin-1 immunoreactivity in the central nervous system and this effect was prevented by either CB1R or CB2R antagonist pretreatment. Insulin resistance might be related to CB2 receptor activation which was independent from central nesfatin-1 immunoreactivity.Öğe The Effects of Cannabinoid Receptor Agonist and Antagonist in Experimental Myoglobinuric Acute Kidney Injury(Wiley, 2022) Erge, Ummuhan; Arslan, Enver; Metin, Melike Sapmaz; Deger, Ecem Busra; Gunduz, Ozgur; Kaya, Oktay[Abstract Not Available]Öğe Effects of Cannabinoid Receptor Blockers on Serum Nesfatin-1/Nucb2 Levels in REM sleep Deprived Mice(Wiley-Blackwell, 2016) Kaya, Oktay; Yilmaz, M. Elif; Bayram, Sinasi; Gunduz, Ozgur; Ozfidan, Gulnur Kizilay; Ozturk, Levent[Abstract Not Available]Öğe The effects of epidermal growth factor on early burn-wound progression in rats(Wiley, 2020) Kaya, Oktay; Orhan, Erkan; Sapmaz-Metin, Melike; Topcu-Tarladacalisir, Yeter; Gunduz, Ozguer; Aydin, BilgehanAfter burns, protecting tissues by medicines in the zone of stasis reduces the width and depth of injury. This study's goal was to reduce burned tissue damage in the zone of stasis using epidermal growth factor (EGF). Forty-eight Wistar rats were separated into three groups. In all groups, the burn procedure was applied following the comb burn model. In Group 1, no postburn treatment was administered. In Group 2, physiological saline solution (0.3 cc) was injected intradermally and in Group 3, EGF (0.3 cc) was injected intradermally into stasis zone tissues after the burn procedure. Surviving tissue rates were 24.0% in Group 1, 25.3% in Group 2, and 70.2% in Group 3. The average numbers of cells stained with Nrf2, HO-1, and the number of apoptotic cells were 230, 150, and 17.5 in Group 1, 230, 145, and 15.0 in Group 2, and 370, 230, and 0 in Group 3, respectively. Values in Group 3 were found to be statistically significantly different than those of Groups 1 and 2; there was no difference between Groups 1 and 2. This study shows that EGF protects zone of stasis tissue from burn damage.Öğe The Effects of Irisin on Experimental Hypertension in Rats(Wiley, 2017) Aydogdu, Nurettin; Tastekin, Ebru; Tayfur, Pinar; Yavuz, Ozlem Yalcinkaya; Kaya, Oktay; Kandemir, Nihayet[Abstract Not Available]Öğe The Effects of Irisin on N?-Nitro-L-arginine Methyl Ester Hydrochloride-Induced Hypertension in Rats(2019) Aydoğdu, Nurettin; Yavuz, Özlem Yalçınkaya; Taştekin, Ebru; Tayfur, Pınar; Kaya, Oktay; Kandemir, NihayetBackground: The cause of about 95% of hypertension, an important public health problem, is unknown. Intensive studies are underway to understand the physiopathology of hypertension. Irisin, a newly discovered hormone, has been reported to dilate vascular smooth muscle and lower blood pressure acutely. Aims: To investigate the effects of chronic irisin treatment on blood pressure and renal functions in a hypertension model established by nitric oxide synthase inhibition by treatment with N?-nitro-L-arginine methyl ester hydrochloride. Study Design: Animal experimentation. Methods: Male Sprague?Dawley rats were divided into four groups (n=8). Control and irisin groups received an intravenous saline injection, hypertension and hypertension + irisin (hypertension + irisin) groups received 1.5 mg/100 g N?-nitro-L-arginine methyl ester hydrochloride. N?-nitro-L-arginine methyl ester hydrochloride (150 mg/L) was added to the drinking water of rats in groups hypertension and hypertension + irisin for three weeks. In the second week of the experiment, irisin (50 nmol/day) was given to rats in groups irisin and hypertension + irisin, and saline was administered to rats in groups control and hypertension for two weeks through subcutaneously placed osmotic minipumps. Blood pressure was measured by the tail-cuff plethysmography method. On the twenty-first day of the experiment, 24-hour urine, blood, and both kidneys of the rats were collected. Results: The hypertension group had elevated systolic, diastolic, and mean arterial blood pressure values compared with the control group, with decreased glutathione levels in tissue and serum, but an increase in serum oxidized glutathione level (p<0.05). Histopathologically, increased tubular injury, cast formation, glomerular sclerosis, and peritubular fibrosis levels were observed (p<0.05). Irisin treatment did not cause any significant change in blood pressure, renal functions, and injury scores. However, renal nitric oxide levels significantly increased, and endothelial nitric oxide synthase immunoreactivity was determined to be reduced (p<0.05). Conclusion: Treatment with chronic irisin at a physiological dose does not reduce blood pressure in an experimental model of hypertension. In different models of experimental hypertension, the effects of irisin administration at different doses and at different periods should be thoroughly investigated.Öğe Effects of Losartan on Glycerol-induced Myoglobinuric Acute Renal Failure in Rats(Kafkas Univ, Veteriner Fakultesi Dergisi, 2013) Kaya, Oktay; Aydogdu, Nurettin; Tastekin, Ebru; Karadag, Cetin Hakan; Gunduz, Ozgur; Sut, NecdetMyoglobinuric acute renal failure (mARF) is an uremic syndrome which develops due to damage of skeletal muscle. It was demonstrated that free radicals and nitric oxide (NO) play an important role in pathogenesis of mARF. Our aim was to investigate the effect of losartan, a drug known for its antioxidant effect, on mARF. In our study, a total of 34 male Spraque Dawley rats were divided into four groups. 1st and 2nd groups were injected with saline, 3rd and 4th groups were injected with intramuscular glycerol. One and 24 hours later, 1st and 3rd groups received saline orally and 2nd and 4th groups have taken 10 mg/kg losartan. Urine was collected; the blood samples and kidneys of the rats were taken under the anesthesia. The levels of NO, arginine, asymmetric dimethylarginine (ADMA), glutathione (GSH) and malondialdehyde (MDA) were determined, renal functions and histopathological changes examined. In our study, we found that levels of urea, creatinine, and potassium, in serum samples and MDA and ADMA in renal tissue were increased in 3rd group when it's compared with the 1st group. Levels of sodium, arginine in serum samples and arginine in renal tissue were reduced 3rd group when compared with the 1st group. When 3rd and 4th groups were compared, serum creatinine was higher in the latter group whereas ADMA level in renal tissue was lower in the same group. We think that there is no positive effect of losartan on the pathogenesis of mARF.Öğe Evaluation of the Effects of High Intensity Interval Training on Cytokine Levels and Clinical Course in Treatment of Opioid Use Disorder(Turkiye Sinir Ve Ruh Sagligi Dernegi, 2020) Durmus, Pelin Tas; Vardar, Mehmet Erdal; Kaya, Oktay; Tayfur, Pinar; Sut, Necdet; Vardar, Selma ArzuObjective: Opioid use disorder (OpUD) is a biological and psychosocial disorder with limited treatment options. Addition of physical exercise to the pharmacological treatment has been proposed to be effective on reducing substance use and improving the quality of life. In this study we aimed to investigate the effects of a high-intensity interval training (HIIT) program on the serum levels of cortisol, insulin-like growth factor1 (IGF-1), interferon-gamma (IFN-gamma), interleukin 17 (IL-17) and the clinical progress of inpatients with OpUD. Method: Our study enrolled 22 male inpatients diagnosed with OpUD on the basis of the DSM-5 criteria. Two groups of 11 individuals were formed as the exercise (EG) and the control (CG) groups. The EG conducted 5 sessions of a HIIT. Participant data were collected with Sociodemographic Questionnaire, the Addiction Profile Index (API), and the Barratt Impulsiveness Scale (BIS-11). Also, the Hamilton Depression Rating Scale (HAM-D), the Hamilton Anxiety Rating Scale (HAM-A) and the Substance Craving Scale (SCS) were used before and after the treatment program in order to evaluate the clinical progress. Blood samples were collected on the 5th and the 21st days for estimation of the serum cortisol, IGF-1, IFN-gamma and the IL-17 levels. Results: Comparison of the pre- and the post-treatment performances of the two groups on the HAM-D, the HAM-A and the SCS indicated a significant drop in the respective scores of the EG. Also, a significant increase was observed in the post-treatment IGF-1 level of the EG as compared to the CG. No differences were observed between the cortisol, IFN-gamma and IL-17 levels of the EG and the CG. Conclusion: HIIT resulted in significant reduction in the symptoms of depression, anxiety and substance craving, and increased the serum IGF-1 levels. HIIT did not change serum cortisol, IFN-gamma and IL-17 levels. We believe this research will contribute to the literature on the treatment of opioid depencence by emphasising the effects of HIIT on patients treated for OpUD.Öğe FARELERDE SKOPOLAMİNLE BOZULMUŞ ÖĞRENME VE BELLEK ÜZERİNENÖROTENSİN AGONİSTİ PD149163’ÜN ETKİSİ(2021) Kaya, Oktay; Topuz, Ruhan DenizNörotensin (NT), santral sinir sisteminde nöromodülatör, nörotransmitter ve nörohormon olarak görev yapan bir tridekapeptittir. NT; vücut sıcaklığının düzenlenmesi, ağrı, motor aktivite, öğrenme ve bellek yapılanması gibi fizyolojik süreçlerde rol oynar. Bu çalışmada NT agonisti PD149163’ün akut ve kronik kullanımının skopolaminle oluşturulmuş bellek bozukluğu üzerine etkilerinin incelenmesi amaçlandı. Bu çalışma Trakya Üniversitesi Hayvan Deneyleri Yerel Etik kurulundan 2019.02.01 karar no ile onaylanmıştır. Çalışmada toplam 32 adet Balb/c türü erişkin erkek fare 4 gruba ayrıldı. Öğrenme ve bellek fonksiyonları Morris su labirenti testinde değerlendirildi. 7 gün boyunca skopolamin (1 mg/kg i.p.) uygulandıktan sonra akut ve kronik (7 gün, 4 mg/kg, i.p) PD149163 tedavisinin öğrenme ve bellek bozukluğunu geri döndürüp döndürmediği incelendi. Çalışmada yüzme eğitimlerinden elde edilen veriler, tekrarlayan ölçümler iki yönlü varyans analizi (ANOVA ) ile analiz edildi. Probe verileri tek yönlü varyans analizi ile değerlendirildi. Kronik PD149163 uygulamasının Morris su labirenti testinde ne öğrenme fazında ne de bellek fazında iyileştirici bir etkisi olmadı. Akut PD149163 uygulamasının skopolaminle oluşturulmuş bellek bozukluğu üzerine herhangi bir etkisi olmadı. Çalışmamızda NT agonistinin skopolaminle oluşturulan öğrenme ve bellek bozukluğunda düzeltici etkisi görülmemiştir. NT reseptörleri yeni ilaç hedefleri olarak değerlendirilmelerine karşın bu alanda yapılacak çok fazla sayıda çalışmaya ihtiyaç olduğu düşüncesindeyiz.
