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    The efficacy of vitamin E in the prevention of lung ischemia-reperfusion injury after cardiopulmonary bypass in open heart surgery
    (Turkiye Klinikleri, 2013) Acipayam M.; Sunar H.; Canbaz S.; Hüseyinova G.; Erbaş H.; Erten O.; Duran E.
    Objective: The purpose of the study was to investigate the effects of vitamin E in the protection of the lung from potential ischemia-reperfusion injury during elective coronary artery bypass graft surgery. Material and Methods: This controlled randomized single-center study included patients who underwent elective coronary bypass grafting (CABG) operation. Forty-nine patients were randomly divided into 2 groups. Water soluble Vitamin E (100 mg) in tepid saline (n=25) or tepid saline alone (n=24) was administered via the jugular vein before the aortic cross clamping. Serum total antioxidant capacity (TAC) levels and serum malonedialdehyde levels (MDA) were measured. Pulmonary biopsies were obtained before the aortic cross clamping and 60 minutes after removing the cross clamp. Biopsies were examined histopathologically under electron microscopy. Results: Serum MDA levels at T1 (15 minutes after removal of the cross clamp) and T2 (30 minutes after removal of the cross clamp) were higher in the control group compared to the Vitamin group. Serum TAC levels at T1, T2 and T3 (60 minutes after removal of the cross clamp) were higher in the Vitamin E group compared to the control group. Histopathologic injury grade was lower in the Vitamin E group than in the control group. Conclusion: Vitamin E was found to be protective against reperfusion induced oxidative injury in the early operative period. © 2013 by Türkiye Klinikleri.
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    Some ultrastructural observations on calcium oxalate raphide crystal idioblasts and meristematic cells of the adventive root tips of Sternbergia lutea (L.) Ker-Gawl. ex Sprengel (Amaryllidaceae)
    (2000) Dane F.; Meriç C.; Hüseyinova G.
    The adventive root tip cells of a bulbous plant. Sternbergia lutea (L.) Ker-Gawl. ex Sprengel (Amaryllidaceae), were examined by LM (light microscope) and EM (electron microscope). At the meristematic area of the root tips, which was 1 mm in length, two types of cell were observed: meristematic cells and Ca-oxalate raphide crystal idioblasts. Ca-oxalate raphide crystal idioblasts were observed to lie in two lines between the inner and outer ground meristems. Some similarities and some differences were found between those cells in their ultrastructure. It was seen that the membrane thickness of crystal idioblasts and meristematic cells were the same. They were also found to be similar in cell content, both containing nuclei, cytoplasm, vacuoles, mitochondria and proplastids. However the organization of the cell contents, the numbers, the forms and sizes of vacuoles were different between these cell types. In some meristematic cells and idioblasts of certain roots, some degenerated changes were seen due to necrosis. Those changes were in the form of plasmolysis. karyolysis, karyorrhexis. chromatin roughness, karyopyknosis and organelle loss.
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    Study of ultrastructural changes on the cochleae caused by various intonations used in classical music
    (2008) Mamedova L.; Kanter M.; Güner S.S.; Bulut E.; Mercantepe T.; Metin I.; Hüseyinova G.
    The aim of this study is to investigate the differences on ultrastructure of the cochleae caused by different classic musical opuses with different intonations. Guinea pigs were grouped into 3, one of which was the control and the other two were the experimental groups. While the first group, which was the control, was not exposed to any music, the second group was exposed to classic musical opuses with extensive intervals (40 decibel) and third group was exposed to classical music opuses with strained intonations (60 decibel) for 6 h a day with 15 min-intervals for totally 10 days. Cochleae tissue samples were taken from the guinea pigs at the end of the tenth day. They were examined at the electron microscopic level. In addition to compansatris processes on the cochleae, thickening on the stereocilias of hair cells and basal membranes and proliferation on the synaptic terminalles of afferent nerves caused by extensive intonations were observed. Extremely obvious degenerative differences such as damage in neuroepitelial cells, nerves and synaptic terminalles as well as compansatris processes caused by strained intonations were determined. As a result of all these observations it was concluded that continuously listening to the strained intonations used in musical opuses has a very harmful effect on the auditory system. © 2008 Academic Journals Inc.