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Öğe Anti-inflammatory and antioxidant effects of infliximab in a rat model of intestinal ischemia/reperfusion injury(Sage Publications Inc, 2012) Pergel, Ahmet; Kanter, Mehmet; Yucel, Ahmet Fikret; Aydin, Ibrahim; Erboga, Mustafa; Guzel, AhmetThe aim of this study was to investigate the possible protective effects of infliximab on oxidative stress, cell proliferation and apoptosis in the rat intestinal mucosa after ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: sham, I/R and I/R+ infliximab; each group comprised 10 animals. Sham group animals underwent laparotomy without I/R injury. I/R groups after undergoing laparotomy, 1 hour of superior mesenteric artery ligation occurred, which was followed by 1 hour of reperfusion. In the infliximab group, 3 days before I/R, infliximab (3 mg/kg) was administered intravenously. All animals were killed at the end of reperfusion and intestinal tissues samples were obtained for biochemical and histopathological investigation in all groups. To date, no biochemical and histopathological changes have been reported regarding intestinal I/R injury in rats due to infliximab treatment. Infliximab treatment significantly decreased the elevated tissue malondialdehyde levels and increased reduced superoxide dismutase and glutathione peroxidase enzyme activities in intestinal tissues samples. I/R caused severe histopathological injury including mucosal erosions, inflammatory cell infiltration, necrosis, hemorrhage, and villous congestion. Infliximab treatment significantly attenuated the severity of intestinal I/R injury, inhibiting I/R-induced apoptosis, and cell proliferation. Because of its anti-inflammatory and antioxidant effects, infliximab pretreatment may have protective effects on the experimental intestinal I/R model of rats.Öğe Anti-inflammatory and antioxidant effects of infliximab on acute lung injury in a rat model of intestinal ischemia/reperfusion(Springer, 2012) Guzel, Ahmet; Kanter, Mehmet; Guzel, Aygul; Pergel, Ahmet; Erboga, MustafaThe purpose of this study was to investigate the role of infliximab on acute lung injury induced by intestinal ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: sham, I/R and I/R+ infliximab; each group contain 10 animals. Sham group animals underwent laparotomy without I/R injury. After I/R groups animals underwent laparotomy, 1 h of superior mesenteric artery ligation were followed by 1 h of reperfusion. In the infliximab group, 3 days before I/R, infliximab (3 mg/kg) was administered by intravenously. All animals were sacrificed at the end of reperfusion and lung tissues samples were obtained for biochemical and histopathological investigation in all groups. To date, no more biochemical and histopathological changes on intestinal I/R injury in rats by infliximab treatment have been reported. Infliximab treatment significantly decreased the elevated tissue malondialdehyde levels and increased of reduced superoxide dismutase, and glutathione peroxidase enzyme activities in lung tissues samples. Intestinal I/R caused severe histopathological injury including edema, hemorrhage, increased thickness of the alveolar wall and a great number of inflammatory cells that infiltrated the interstitium and alveoli. Infliximab treatment significantly attenuated the severity of intestinal I/R injury. Furthermore, there is a significant reduction in the activity of inducible nitric oxide synthase and arise in the expression of surfactant protein D in lung tissue of acute lung injury induced by intestinal I/R with infliximab therapy. It was concluded that infliximab treatment might be beneficial in acute lung injury, therefore, shows potential for clinical use. Because of its anti-inflammatory and antioxidant effects, infliximab pretreatment may have protective effects in acute lung injury induced by intestinal I/R.Öğe The comparison of single-dose ceftriaxone, five-day azithromycin, and ten-day amoxicillin/clavulanate for the treatment of children with acute otitis media(Turkish J Pediatrics, 2007) Biner, Betuel; Celtik, Coskun; Oner, Naci; Kucukugurluoglu, Yasemin; Guzel, Ahmet; Yildirim, Cetin; Adali, Mustafa KemalThe aim of the study was to evaluate the efficacy of short-course antimicrobial therapies [single intramuscular dose of ceftriaxone (50 mg/kg, not exceeding 1 g), 5 days of azithromycin (10 mg/kg on day 1, then 5 mg/kg daily on days 2-5) and the traditional 10-day course of amoxicillin/clavulanate (90/6.4 mg/kg/day in 2 doses)] in children with acute otitis media (AOM). The study was conducted as a prospective, comparative, open randomized trial between February 2001 and April 2003, and 104 children were enrolled, with a mean age of 3.8 (2.3) years. The clinical and otoscopic assessments of the children were made on days 0, 3, 11 and 30 after admission, and tympanometry was performed on day 30. The patients were diagnosed and followed with a scoring system. Clinical success was achieved in 29/34 patients (85.3%) in the ceftriaxone group, 27/31 patients (87.1%) in the azithromycin group and 34/39 children (87.2%) in the arnoxicillin/clavulanate group. The rate of persistence of middle-ear fluid did not differ between the three groups (p>0.05). During the one-month period, no recurrent case was observed. The most common drug-related adverse effects were associated with the gastrointestinal system. In conclusion, for the treatment of children with AOM, the clinical success of single-dose intramuscular ceftriaxone and of five-day azithromycin treatments was comparable to that of the traditional 10-day therapy with high-dose arnoxicillin/clavulanate.Öğe A cost analysis of radiologic imaging in pediatric trauma patients(Turkish Assoc Trauma Emergency Surgery, 2010) Guzel, Ahmet; Temizoz, Osman; Aksu, Burhan; Sut, Necdet; Karasalihoglu, SerapBACKGROUND The aim of this study was to examine the cost of radiologic imaging in pediatric trauma patients admitted to the pediatric emergency department. METHODS All patients were retrospectively evaluated according to age, gender, details of injury, radiological investigations ordered and their results, cost of radiologic imaging, length of stay, outcome of the injury, and hospitalization rates. RESULTS The cost of radiologic imaging was retrospectively analyzed in 1231 trauma patients aged between one month and 15 years (mean 5.91 +/- 3.82 years). For the 996 patients who had radiological imaging, 3382 images were taken in total. Of these, only 300 (8.8%) were abnormal. The mean (and SD) total cost of radiologic imaging was US$ 40.42 ($34.38) (range $4.67 to $139.26). Total cost correlated inversely with Glasgow Coma Scale (GCS) (r=-0.37, p<0.001), directly with Injury Severity Score (ISS) (r=0.27, p<0.001) and was not correlated with the Pediatric Trauma Score (PTS) (r=-0.16, p>0.05). The mean (and SD) duration of hospital stays was 8.54 (10.91) hours. CONCLUSION Advanced radiological images may help in early diagnosis of trauma cases. However, periodic education programs to prevent unnecessary radiological imaging in emergency departments are also necessary to decrease the cost of these imaging modalities.Öğe Dermatological Emergencies in Childhood: 5 Years Experience(Aves, 2009) Guzel, Ahmet; Karasalihoglu, Serap; Sayar, Esra Hazar; Arican, Ozer; Kavalci, CemilBackground and design: The aim of this study was to examine clinical and demographic characteristics of patients who presented with dermatological lesions to the pediatric emergency department. Materials and Methods: All patients who presented with the complaint of dermatological problems within the last 5 years were evaluated retrospectively. Results: Of 498 patients applied to our clinic, 273 (54.8%) were males, 225 (45.2%) were females. The median age of the patients was 5.56 +/- 3.72 years. Our study included four (0.8%) neonates, seventy (14.1%) infants, one hundred sixty one (32.3%) preschool and two hundred sixty three (52.8%) school going children who were applied for the management of dermatological emergencies. The most common dermatological diseases were infectious skin disorders, urticaria, and drug reactions. Systemic therapy was prescribed in 66.9% and local therapy in 9.9% of the patients. Combined local and systemic therapy was given to 17.3% of the cases. Of all patients, 23 (4.6%) were consulted by dermatology. Of all patients, 20.9% were admitted to our hospital, while 78.9% treated with outpatient and one patient left hospital before completion of his therapy. Conclusion: The most common dermatological diseases are infectious skin disorders in pediatric emergency department. Common skin diseases in pediatric emergency department must be established and periodical education programs associated with these diseases were planned.Öğe Edirne Basic Education Module: The Result and the Evaluation of Activity for Three Year(Emergency Medicine Physicians Assoc Turkey, 2009) Kavalci, Cemil; Guzel, Ahmet; Cevik, Yunsur; Durukan, PolatBACKGROUND: Physicians should be improved their knowledge and ability due to represent for equal and reliable health setting. We aimed to present the three-year evaluation results of the Basic Education Module, which is the first step of the certification program for physicians in Edirne. MATERIAL AND METHODS: All physicians were performed with test contained twenty questions before and after of course. The differences between the values before and after course were tested with depend and independent t test. A p value less than 0.05 was accepted as the level of significance. RESULTS: Two hundred seventy-seven physicians were included in this study. Differences in values of all physicians which admitted to education programs between before and after course in were statistically significant (p <0.001). Although differences in values of control groups between before and after course in were not statistically significant (p >0.05). CONCLUSION: Basic Education Module programs are necessary to represent for quality and reliable health setting by all physiciansÖğe The effect of hyperbaric oxygen treatment on aspiration pneumonia(Springer, 2011) Sahin, Sevtap Hekimoglu; Kanter, Mehmet; Ayvaz, Suleyman; Colak, Alkin; Aksu, Burhan; Guzel, Ahmet; Basaran, Umit NusretWe have studied whether hyperbaric oxygen (HBO) prevents different pulmonary aspiration materials-induced lung injury in rats. The experiments were designed in 60 Sprague-Dawley rats, ranging in weight from 250 to 300 g, randomly allotted into one of six groups (n = 10): saline control, Biosorb Energy Plus (BIO), hydrochloric acid (HCl), saline + HBO treated, BIO + HBO treated, and HCl + HBO treated. Saline, BIO, HCl were injected into the lungs in a volume of 2 ml/kg. A total of seven HBO sessions were performed at 2,4 atm 100% oxygen for 90 min at 6-h intervals. Seven days later, rats were sacrificed, and both lungs in all groups were examined biochemically and histopathologically. Our findings show that HBO inhibits the inflammatory response reducing significantly (P < 0.05) peribronchial inflammatory cell infiltration, alveolar septal infiltration, alveolar edema, alveolar exudate, alveolar histiocytes, interstitial fibrosis, granuloma, and necrosis formation in different pulmonary aspiration models. Pulmonar aspiration significantly increased the tissue HP content, malondialdehyde (MDA) levels and decreased (P < 0.05) the antioxidant enzyme (SOD, GSH-Px) activities. HBO treatment significantly (P < 0.05) decreased the elevated tissue HP content, and MDA levels and prevented inhibition of SOD, and GSH-Px (P < 0.05) enzymes in the tissues. Furthermore, there is a significant reduction in the activity of inducible nitric oxide synthase, TUNEL and arise in the expression of surfactant protein D in lung tissue of different pulmonary aspiration models with HBO therapy. It was concluded that HBO treatment might be beneficial in lung injury, therefore, shows potential for clinical use.Öğe Effects of methylene blue in reducing cholestatic oxidative stress and hepatic damage after bile-duct ligation in rats(Elsevier Gmbh, Urban & Fischer Verlag, 2010) Aksu, Burhan; Umit, Hasan; Kanter, Mehmet; Guzel, Ahmet; Aktas, Cevat; Civelek, Sabiha; Uzun, HafizeThe aim of this study was to evaluate the effects of methylene blue against cholestatic oxidative stress and liver damage after ligation of the common bile duct in male Wistar rats. Eight animals were included in each of the following five groups: untreated control, methylene blue control, sham-operated, bile-duct ligation, and bile-duct ligation plus methylene blue. Methylene blue was administered intraperitoneally for 14 days at a daily dose of 2 mg/kg per day. All rats were sacrificed 2 weeks following the experimental treatment and the livers of all groups were examined biochemically and histopathologically. The severity of cholestasis and hepatic injury were determined by changes in the plasma, including enzymatic activities: aspartate aminotransferase, alanine aminotransferase, gamma glutamine transferase, and also bilirubin levels. Malondialdehyde, nitric oxide and superoxide dismutase were measured to indicate the oxidative status in the liver tissue. Myeloperoxidase activity and levels of tissue hydroxyproline were determined as measures of neutrophil activation and collagen accumulation, respectively. Liver damage was significantly prevented in the bile-duct ligated rats treated with methylene blue compared with the control bile-duct ligated rats without methylene blue. Treatment with methylene blue markedly reduced activities of serum transaminase, gamma glutamine transferase and bilirubin levels as compared to bile-duct ligated rats without methylene blue. Positive immunolabelling for alpha-smooth muscle actin (alpha-SMA) was increased, especially in vascular smooth muscle cells, fibrotic septa and also around the proliferated bile ducts, after bile-duct ligation. Only weak alpha-SMA immunolabelling was seen in livers of rats treated with methylene blue. These results indicate that methylene blue can attenuate hepatic damage in extrahepatic cholestasis by reducing oxidative stress and inflammatory processes. (C) 2008 Elsevier GmbH. All rights reserved.Öğe Effects of sphingosylphosphorylcholine against cholestatic oxidative stress and liver damage in the common bile duct ligated rats(W B Saunders Co-Elsevier Inc, 2009) Aksu, Burhan; Umit, Hasan; Kanter, Mehmet; Guzel, Ahmet; Inan, Mustafa; Civelek, Sabiha; Aktas, CevatThe goal of this study was to evaluate the possible protective effects of sphingosylphosphorylcholine (SPC) against cholestatic oxidative stress and liver damage in the common bile duct ligated rats. Fifty-six animals were included in each of the following 7 groups: control, SPC control, phosphate-buffered solution control, sham operated, bile duct ligation (BDL), BDL plus phosphate-buffered solution, and BDL plus SPC. Sphingosylphosphorylcholine was administered 14 days at a daily dose of 2 mu m/mL intraperitoneally. The severity of cholestasis and hepatic injury was determined by changes in the plasma enzyme activities of aspartate aminotransferase, alanine aminotransferase, gama glutamin transferase, and levels of total bilirubin and direct bilirubin. Malondialdehyde, nitric oxide, and superoxide dismutase were determined to evaluate the oxidative status in the liver tissue. Myeloperoxidase activity and levels of tissue hydroxyproline were determined to assess neutrophil activation and collagen accumulation, respectively. Treatment with SPC markedly reduced serum transaminase activities as compared to BDL rats. Sphingosylphosphorylcholine also inhibited the increase in liver malondialdehyde; nitric oxide levels significantly and also attenuated the depletion of superoxide dismutase in the liver after BDL. Similarly, the increase in tissue myeloperoxidase activity and hydroxyproline owing to BDL was also attenuated by the SPC treatment. These data were supported by histopathologic findings. The a-smooth muscle actin-positive cells in the BDL were observed to be reduced with the SPC treatment. In conclusion, these findings suggested that SPC can attenuate hepatic damage in extrahepatic cholestasis by prevention of oxidative stress, and inflammatory process. All these findings suggest that SPC may be a promising new therapeutic agent for cholestatic liver injury. (C) 2009 Elsevier Inc. All rights reserved.Öğe Evaluation of the fall-related trauma cases applied to our pediatric emergency department(Turkish Assoc Trauma Emergency Surgery, 2007) Guzel, Ahmet; Karasalihoglu, Serap; Kucukugurluoglu, YaseminBACKGROUND The aim of this study was to evaluate the fall-related trauma cases who were admitted to our pediatric emergency unit. METHODS We retrospectively evaluated the files of 161 cases (100 males [62.1%], 61 females [37.9%]) who had sustained falls and were admitted to our pediatric emergency unit. Data on the patients' age, sex, arrival type, type of fall, height fallen, type of injuries, radiological findings and hospitalization rates were investigated. RESULTS Of 161 cases mean age was 4.29 +/- 3.33 years and height fallen was 135 +/- 95 centimeters. The common types of fall were from furniture (35.4%), stairs (17.4%) and balconies and windows (11.2%). Major injuries included head trauma (84.4%), soft tissue (72.7%) and extremity (37.9%). Thirty cases (18.6%) were hospitalized, while 112 cases (69.6%) were treated as outpatient and 11.8% of them were discharged due to their own demand. CONCLUSION The majority of fall-related injuries in childhood mostly occurs in private houses and head trauma is the most frequent injury. To decrease the frequency of the fall injuries, strategies should include parents' education about the mechanism of falls and increase prevention strategies.Öğe Evaluation of Triage Tox Drug Kit in Intoxication Cases(Galenos Yayincilik, 2009) Kavalei, Cemil; Guzel, Ahmet; Cevik, Yunsur; Durukan, PolatPoisonings are important Causes of emergency room visits It especially very common among adolescents and women. in this study we airved to define the value of tox drug screen kit in the diagnosis and treatment of poisoning cases. Poisoning cases admitted to emergency department of Trakya University Hospital between january and June 2009 were evaluated prospectively. Demographic features of patients poisoning agents of intake of poisonous agent admission times treatment methods results of toxicologic screen durations of emergency department treatment and outcomes were recorded. Data were expressed as mean +/- standard deviation median and percentage. During the study period. 1.7% of emergency department visits were due to poisoning. 100 patients were included in the study. Mean age of the patients was 24.3 +/- 9.2 years. 32 of the patients were male (32%) and 68 were female (68%). Mean admission time to the hospital was 187.6 +/- 146.8 minutes. It was detected that 30% of the patients were poisoned with a single agent. The most common poisoning agents were tablets (94%). 52% of the patients were discharged from the hospital, 48% were hospitaliceed. No deaths were observed. We suggest the tox drug screen Kit is useful in the diagnosis and treatment of poisoning.Öğe A fat headache(New Zealand Medical Assoc, 2009) Karasalihoglu, Serap; Guzel, Ahmet; Ugurluoglu, Yasemin Kucuk; Unlu, Ercuement[Abstract Not Available]Öğe Fatal Acute Diclofenac-Induced Rhabdomyolysis in A Pediatric Patient(Galenos Publ House, 2011) Guzel, Ahmet; Biner, Betul Orhaner; Karasalihoglu, Serap; Aylanc, HakanDiclofenac, a widely used nonsteroidal anti-inflammatory drug, has been reported to cause fatal drug-induced rhabdomyolysis very rarely. We report a case of a 13-month-old girl who developed fatal rhabdomyolysis after administration of intramuscular diclofenac for relief of pain caused by a scalding burn injury. Rhabdomyolysis due to diclofenac has not been reported in children. Here, we present the first case report to demonstrate rhabdomylysis secondary to diclofenac administration.Öğe Indications for Brain Computed Tomography and Hospital Admission in Pediatric Patients with Minor Head Injury: How Much Can We Rely upon Clinical Findings?(Karger, 2009) Guzel, Ahmet; Hicdonmez, Tufan; Temizoz, Osman; Aksu, Burhan; Aylanc, Hakan; Karasalihoglu, SerapObjectives: The aim of this study was to describe the characteristics of patients with a minor head injury (MHI) who were admitted to a pediatric emergency unit and to identify the clinical signs and symptoms that most reliably predict the need for cranial computed tomography (CCT) and hospital admission following MHI. Methods: All patients were retrospectively evaluated according to age, gender, details of injury, presenting symptoms, physical examination findings, radiological investigations ordered and results, length of stay, outcome of the injury and hospitalization rates. Results: The factors affecting indications for computed tomography and hospitalization were retrospectively analyzed in 916 patients - 585 males and 331 females, aged between 1 month and 15 years (mean: 5.01 +/- 3.58 years), with MHI. A multivariate analysis revealed significant correlations between CCT abnormalities and Glasgow Coma Scale scores of 13 or 14, headache, posttraumatic amnesia, blurred vision, cephalohematomas, periorbital ecchymoses, otorrhea and abnormal neurological findings. CCT abnormalities were identified in 67 (19.8%) of the 338 CCT scans. Twenty of the 67 patients (29.9%) with CCT scan abnormality had no clinical signs. Of all cases, 125 (13.6%) were hospitalized, 617 (67.4%) were treated as outpatients, and 174 (19.0%) left the emergency department based on a personal decision. Conclusion: Some clinical risk factors can be used as predictors of abnormalities in CCT scans following MHI, but the absence of such clinical findings does not exclude the possibility of intracranial injuries. Copyright (C) 2009 S. Karger AG, BaselÖğe Post-Operative Malignant Hyperthermia in a Child after Colon Interposition(Aves, 2015) Sahin, Sevtap Hekimoglu; Inan, Mustafa; Aksu, Burhan; Oner, Naci; Colak, Alkin; Guzel, AhmetMalignant hyperthermia (MH) is a rare and potentially life threatening fatal complication of anaesthesia. We present a 2-year-old boy with late onset MH after colon interposition to replace the oesophagus under sevoflurane anaesthesia. The patient was treated with intravenous dantrolene sodium as well as cooling and controlled ventilation. Despite treatment, the patient developed cardiopulmonary arrest at 21 hours after the operation and died. It should be kept in mind that post-operative MH may develop during these types of operations with ischaemia-reperfusion injuries.Öğe Preventive effects of curcumin on different aspiration material-induced lung injury in rats(Springer, 2009) Guzel, Ahmet; Kanter, Mehmet; Aksu, Burhan; Basaran, Umit Nusret; Yalcin, Oemer; Guzel, Aygul; Uzun, HafiseWe have studied whether curcumin protects different pulmonary aspiration material-induced lung injury in rats. The experiments were designed in 60 Sprague-Dawley rats, randomly allotted into one of six groups (n = 10): normal saline (NS, control), enteral formula (Biosorb Energy Plus, BIO), hydrochloric acid (HCl), NS + curcumin-treated, BIO + curcumin-treated, and HCl + curcumin-treated. NS, BIO, HCl were injected in to the lungs. The rats received curcumin twice daily only for 7 days. Seven days later, both lungs in all groups were examined histopathologically, immunohistochemically, and biochemically. Histopathologic examination was performed according to the presence of peribronchial inflammatory cell infiltration, alveolar septal infiltration, alveolar edema, alveolar exudate, alveolar histiocytes, interstitial fibrosis, granuloma, and necrosis formation. Immunohistochemical assessments were examined for the activity of inducible nitric oxide synthase (iNOS) and the expression of surfactant protein D (SP-D). Malondialdehyde (MDA), hydroxyproline (HP), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity were measured in the lung tissue. Our findings show that curcumin inhibits the inflammatory response reducing significantly (P < 0.05) all histopathological parameters in different pulmonary aspiration models. Pulmonary aspiration significantly increased the tissue HP content, MDA levels and decreased the antioxidant enzyme (SOD, GSH-Px) activities. Curcumin treatment significantly decreased the elevated tissue HP content, and MDA levels and prevented inhibition of SOD, and GSH-Px enzymes in the tissues. Furthermore, our data suggest that there is a significant reduction in the activity of iNOS and a rise in the expression of SP-D in lung tissue of different pulmonary aspiration models with curcumin therapy. Our findings support the use of curcumin as a potential therapeutic agent in acute lung injury.Öğe Protective effect of curcumin on acute lung injury induced by intestinal ischaemia/reperfusion(Sage Publications Inc, 2013) Guzel, Aygul; Kanter, Mehmet; Guzel, Ahmet; Yucel, Ahmet Fikret; Erboga, MustafaThe aim of this study is to evaluate the role of curcumin on acute lung injury induced by intestinal ischaemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into 3 groups: sham, I/R, and I/R + curcumin; each group contains 10 animals. Sham group animals underwent laparotomy without I/R injury. After I/R groups animals underwent laparotomy, 1 h of superior mesenteric artery ligation were followed by 1 h of reperfusion. In the curcumin group, 3 days before I/R, curcumin (100 mg/kg) was administered by gastric gavage. All animals were killed at the end of reperfusion and lung tissue samples were obtained for biochemical and histopathological investigation in all groups. To date, no more biochemical and histopathological changes on intestinal I/R injury in rats by curcumin treatment have been reported. Curcumin treatment significantly decreased the elevated tissue malondialdehyde levels and increased reduced superoxide dismutase, and glutathione peroxidase enzyme activities in lung tissue samples. Intestinal I/R caused severe histopathological injury including oedema, haemorrhage, increased thickness of the alveolar wall, and infiltration of inflammatory cells into alveolar spaces. Curcumin treatment significantly attenuated the severity of intestinal I/R injury. Furthermore, there is a significant reduction in the activity of inducible nitric oxide synthase and increase in the expression of surfactant protein D in lung tissue of acute lung injury induced by intestinal I/R with curcumin therapy. It was concluded that curcumin treatment may have beneficial effects in acute lung injury, and therefore has potential for clinical use.Öğe Protective effects of S-methylisothiourea sulfate on different aspiration materials-induced lung injury in rats(Elsevier Ireland Ltd, 2008) Guzel, Ahmet; Basaran, Umit Nusret; Aksu, Burhan; Kanter, Mehmet; Yalcin, Omer; Aktas, Cevat; Guzel, AygulObjectives: The aim of this study was to evaluate the efficiency of inducible nitric oxide synthase (iNOS) specific inhibitor, S-methylisothiourea sulfate (SMT) in preventing lung injury after different pulmonary aspiration materials in rats. Material and methods: The experiments were performed in 80 Sprague-Dawley rats, ranging in weight from 220 to 250 g, randomly allotted into one of the eight groups (n = 10): normal saline (NS, control), Biosorb Energy Plus (BIO), sucralfate (SUC), hydrochloric acid (HCl), NS + SMT treated, BIO + SMT treated, SUC + SMT treated, and HCl + SMT treated. NS, BIO, SUC, HCl were injected in to the Lungs in a volume of 2 ml/kg. The rats received twice daily intraperitoneal injections of 20 mg(kg day) SMT (Sigma Chemical Co.) for 7 days. Seven days Later, rats were killed, and both lungs in all groups were examined immunohistochemically and histopathologically. Results: Our data show that SMT inhibits the inflammatory response significantly reducing (p < 0.05) peribronchial inflammatory cell infiltration, alveolar septal, infiltration, alveolar edema, alveolar exudate, alveolar histiocytes, interstitial fibrosis, granuloma, and necrosis formation in different pulmonary aspiration models. Furthermore, our data suggest that there is a significant reduction in the activity of MOS and arise in the expression of surfactant protein D in lung tissue of different pulmonary aspiration models with SMT therapy. Conclusion: it was concluded that SMT treatment might be beneficial in Lung injury, therefore shows potential for clinical use. Crown Copyright (c) 2008 Published by Elsevier Ireland Ltd. All rights reserved.Öğe A Rare Cause of Epilepsy: Two Cases of Dyke-Davidoff-Masson Syndrome(Galenos Yayincilik, 2009) Guzel, Ahmet; Gezgin, Tuba; Unlu, Ercument; Vatansever, Binay; Karasalihoglu, SerapDyke-Davidoff-Masson syndrome (DDMS) is clinical characterized by epilepsy, contralateral hemiplegia, hemiparesia, difficulty in learning, mental retardation, cerebral hemiatrophy, in nevroimaging, unilateral calvarial thickening, enlargement of paranasel sinus and increased of aeration in radiologic imaging. Cogenital disorders, infections, vascular diseases, trauma, tumor, ischemic and hemorrmagic reasons have been implicated in the etiology in this report, we discussed as a reason of resistant epilepsy in a10 myear old female and 2 month old male patient with DDMS that has been found comparatively rarerly.Öğe The role of curcumin on intestinal oxidative stress, cell proliferation and apoptosis after ischemia/reperfusion injury in rats(Springer, 2011) Yucel, Ahmet Fikret; Kanter, Mehmet; Pergel, Ahmet; Erboga, Mustafa; Guzel, AhmetThe aim of this study was to demonstrate the role of curcumin on oxidative stress, cell proliferation and apoptosis in the rat intestinal mucosa after ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: sham, I/R and I/R? curcumin; each group contain 10 animals. Sham group animals underwent laparotomy without I/R injury. After I/R groups animals underwent laparotomy, 1 h of superior mesenteric artery ligation were followed by 1 h of reperfusion. In the curcumin group, 3 days before I/R, curcumin (100 mg/kg) was administered by gastric gavage. All animals were sacrificed at the end of reperfusion and intestinal tissues samples were obtained for biochemical and histopathological investigation in all groups. Curcumin treatment significantly decreased the elevated tissue malondialdehyde levels and increased of reduced superoxide dismutase, and glutathione peroxidase enzyme activities in intestinal tissues samples. I/R caused severe histopathological injury including mucosal erosions and villous congestion and hemorrhage. Curcumin treatment significantly attenuated the severity of intestinal I/R injury, with inhibiting of I/R-induced apoptosis and cell proliferation. These results suggest that curcumin treatment has a protective effect against intestinal damage induced by intestinal I/R. This protective effect is possibly due to its ability to inhibit I/R- induced oxidative stress, apoptosis and cell proliferation.
