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Öğe Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells(Walter De Gruyter Gmbh, 2017) Eser, Ferda; Altundag, Ergul Mutlu; Gedik, Gulsah; Demirtas, Ibrahim; Onal, Adem; Selvi, BedrettinAim: D-pinitol, a natural compound has shown various biological and pharmacological effects. Last studies are focused on the determination of its further pharmacological activities including mainly biological activity. Therefore, isolation of D-pinitol from the leaves of Colutea cilicica Boiss et Bal. and investigation of its apoptotic and anti-inflammatory activity on K562 cell lines were aimed in the concept of the study. Materials and methods: Isolation of D-pinitol was performed by column chromatography. Chemical structure of the compound was confirmed by spectroscopic methods including 1H NMR, 13C NMR, 2D NMR, HPLC-TOF/MS, and IR. Cell viability was evaluated by dose and time dependent in K562 cell lines. D-pinitol was isolated from C. cilicica leaves for the first time. Results: Stimulation of cells with D-pinitol (0-80 M) was observed for 24, 48 and 72 h. It is determined that D-pinitol inhibited protein expression of Cox-2 in K562 cells. We observed that Poly (ADP-ribose) polymerase (PARP) protein expression did not change, but Cox-2 protein expression reduced with non-cytotoxic concentrations of D-pinitol. Conclusion: It is concluded that D-pinitol did not affect cell proliferation and apoptosis in K562 cells however reduced the inflammation, significantly. These results show that D-pinitol may be anti-inflammatory agent for the treatment of K562 cells.Öğe Development of bergamot flavors according to their enantiomeric state(Marmara Univ, 2022) Gedik, Gulsah; Gulhan, Nagihan; Elhadef, Ralf; Kutuk, Yasemin; Gokdeniz, Gozde; Yakak, M. Onur; Emirle, HilalIn our study, enantiomers of bergamot essence components were investigated. Afterwards, the flavoring effects of each of its optical isomers were investigated. Our aim is to find and develop the product with the strongest aroma effect. The effects of components that determine the quality of bergamot oil on values such as odor, taste and colour were investigated by gas chromatography-mass spectrometry (GC-MS) and sensory analysis evaluation. Moreover, application studies taken place in which shelf life studies be monitored depending on the use of antioxidants. Application and GC results were examined. In line with these results, it was determined that butylated hydroxy anisole (BHA), caffeic acid and gallic acid kept the product stable until the end of the 18th month.Öğe Effect of gel formulation obtained from Fomes fomentarius on bleeding and clotting time: A pilot study(Istanbul Univ, Fac Pharmacy, 2020) Gedik, Gulsah; Asan, Hulya; Ozyurt, Anil; Alli, Hakan; Asan, Ahmet; Nazli, Hakan; Sarp, OnderBackground and Aims: Fomes fomentarius (L.) Fr. (jar fungus) has been used extensively in the past by surgeons, barbers, dentists, and is therefore called surgeon's fungus. The hemostatic properties of F. fomentarius extracts are important in medicine. The aim of this study was to investigate the effect of the gel formulation obtained from Fames Famentarius on bleeding and clotting time in rat models. Methods: The gel was made for this purpose and a cross-linked polymer of acrylic acid was used as a gelling agent. Results: The outcomes of the characterization analysis indicate the development of a successful gel formulation with optimum characteristics. Data from the antimicrobial study showed a good inhibition zone, with the strongest values against Klebsietta pneumaniae, Candida krusei, C. tropicalis, C. guitliermondii. The gel administration shortened the hemostasis time after bleeding from skin incisions by 63.9% and tail incisions by 55.63% from the original time. For coagulation time, these results are determined as 71.56% from the original time. The gel administration shortened the coagulation time after bleeding from the tail incisions by 69.9% from the original time. Conclusion: Our preliminary study showed that the gel formulation obtained from F. fomentarius is a potent hemostatic agent in a rat-bleeding model.Öğe Formulation and evaluation of intimate wash containing manuka oil and salix bark extract(Marmara Univ, 2022) Gedik, GulsahMost of the feminine hygiene products sold in the market contain heavy chemicals that damage the mucosa. The well-designed intim formulation cleans without damage, supports the flora, protects against dryness, and maintenances of balanced microflora. For this purpose, we designed formulations made with natural raw materials such as manuka oil and Salix alba bark extract and investigated their irritation potential and effectiveness. A healthy vagina is dominated by Lactobacillus that produces lactic acid, resulting in a characteristic acidic environment (pH 34), as it correlates with vaginal health by inhibiting the growth of bacteria and may also play a part in local immune defense. This new manuka oil- salix bark extract containing wash formulation was specifically formulated to achieve a formulation pH of 4.6-4.8 to be compatible with the normal skin pH range and to help maintain vulvovaginal skin homeostasis and provide protection against harmful bacteria. The formulation we designed was used by volunteers for 4 weeks and was found suitable in terms of gentle clean, refreshing feeling, shooting the skin, smell and irritation properties. None of the volunteers have experienced any side effects during product application. Our study findings further support other studies that have found that women who use intim wash attitudes and perceptions about hygiene.Öğe In-vitro evaluation of dendrimeric formulation of oxaliplatin(Taylor & Francis Ltd, 2021) Nazli, Hakan; Gedik, GulsahThe aim of this study is, preparing various dendrimeric formulations of oxaliplatin and investigating their properties. First of all, the solubility enhancement capabilities of polyamidoamine (PAMAM) G3.5 and PAMAM G4.5 dendrimers were investigated. The results showed that oxaliplatin solubility mostly increasing linearly with dendrimer concentration. Additionally, the increase was more notable in PAMAM G4.5 dendrimers. Then, drug-dendrimer complexes were prepared in different mediums, since the medium used can affect the amount of drug-loaded to dendrimers. Prepared complexes were examined for loading capacity and loading efficiency. It was found that PAMAM G4.5 dendrimers can complex with 2- to 5-fold more oxaliplatin than PAMAM G3.5. Finally, oxaliplatin was modified to a platinum (IV) compound to prepare chemical drug-dendrimer conjugates. Ester bonds were established by Steglich esterification through the hydroxyl group of modified oxaliplatin and the carboxyl groups of the dendrimers. The formulations were characterized by UV, IR, NMR spectroscopy, and dynamic light scattering techniques. PAMAM G3.5 conjugate was further evaluated for the cytotoxicity test. The IC50 value of PAMAM G3.5 conjugate was found as 0.72 mu M. For unmodified oxaliplatin, this value was 14.03 mu M. As a result, a dendrimer-based drug delivery system that has been found promising for further improvement has been developed successfully.Öğe Öğe Potential problems of biosimilars in clinical practice(Marmara Univ, Fac Pharmacy, 2016) Korucu, Fatma Ceyda; Nazli, Hakan; Gedik, Gulsah; Cicin, IrfanBiopharmaceuticals are macro and complex medicines obtained from biological sources using biotechnology. In the production of biopharmaceuticals, due to the use of living organisms and the complex method of production, in different series made by the same manufacturer even consist differences. Thus different manufacturers can produce similar molecules to the originator, biosimilar definition is used to indicate these differences. Nowadays biosimilars are used in treatment of many diseases especially cancer, hematologic diseases and endocrine diseases. Using biosimilars in treatment more frequently and enalarge of their market share encourage to produce copies of patent expired products. The objective of studies in the licensing process is to show rather than to prove that biosimilars are better than their originator, in terms of structure, safety and efficiency. Immunogenicity is the biggest problem encountered in clinical practice for biopharmaceutical products and it may affect medicine activity and safety. In clinical practice, health authoritarian should establish well balance of costs, patient benefit, uncertainty.Öğe A Preliminary Screening Study with Dermal Tea Formulations Against 311 nm Ultraviolet B Radiation(Soc Cosmetic Chemists, 2022) Gedik, Gulsah; Ugurlu, TimucinOne potential health benefit of Camellia sinensis extracts on the skin is protection from the detrimental effects of UV radiation. Tea polyphenols both absorb the UV and also alleviate the UV-induced damage in human skin. In this study, we aimed to test the protection of black and green tea gels against UV. The gels were prepared using a carbomer resin and freeze-dried black or green tea extracts. In formulations, total phenolic content, antioxidant activity, and free radical scavenging activity were tested. The sites were irradiated with an artificial narrow band UVB source 30 min after topical application of the formulations on separate regions of the upper back of 21 subjects, Black and green tea gels, and the commercial sunscreen protected the skin of volunteers against the UV erythema. Caffeine gel and carbopol control gels did not provide any protection. Commercial sunscreens can only protect the skin by absorbing or scattering UV radiation. However, tea extracts both absorb the UV radiation and also have the potential to repair the UV damage inside the skin due to their strong antioxidant contents. Tea extracts are safe for humans as well as for aqueous environments without toxicological concerns.
