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Öğe Calcium oxalate crystals during development of male and female gametophyte in Leucojum aestivum (Amaryllidaceae)(2009) Ekici N.; Dane F.In this study, calcium oxalate crystal content of Leucojum aestivum was investigated during its life cycle. Ca-oxalate crystals were observed in adventative roots and leaves in all life cycle of L. aestivum but they were seen in pedicel and tepals from budding to flowering stages. During sporogenesis, gametogenesis and mature embryo sac phase, crystals were observed in ovary, style and tepals. Ca-oxalate crystals were found only at pollen mitosis phase in anthers. It is established that calcium oxalate crystals were of the raphide type.Öğe Calcium oxalate crystals in floral organs of Helianthus annuus L. and H. tuberosus L. (Asteraceae)(2004) Meric C.; Dane F.Helianthus annuus L. and Helianthus tuberosus L. belong to Asteraceae that is one of the greatest families of plant kingdom. Calcium oxalate crystals are found in most organs and tissues of many plant species. The type, morphology and distribution of calcium oxalate crystals in floral organs of H. annuus and H. tuberosus were studied. Crystals were investigated at light and electron microscopy levels. CaOx crystals in calyx and bracts both of H. annuus and H. tuberosus were not observed. The ligulate and tubulate corollas of H. annuus had styloid and prismatic crystals. Also in both of the ligulate and tubulate corollas of H. tuberosus were observed prismatic and styloid crystals as similar with H. annuus. Styloid and prismatic types of CaOx crystals in filaments of H. annuus and H. tuberosus were determined. In endothecial layer and tapetum cells of anthers of both of taxa only styloid type crystals were observed. The ovary was not contains CaOx crystals in H. annuus and H. tuberosus, Style of both of taxa had styloid shape crystals. But in stigma trichomes of H. annuus and H. tuberosus druses were found. The raphides were not observed in both of taxa. This study provides additional knowledge about the presence of CaOx crystals in Asteraceae.Öğe Calcium oxalate crystals in vegetative and floral organs of Galanthus sp. (Amaryllidaceae)(Asian Network for Scientific Information, 2007) Ekici N.; Dane F.In this study Ca-oxalate crystals in vegetative and floral organs of Galanthus plicatus L., Galanthus gracilis L., Galanthus elwesii L. were investigated. The crystals were detected in all vegetative organs but they were only detected in tepal and ovary of floral organs. They were not observed in mature anthers and old adventative roots. Calcium oxalate crystals were of the raphide type. © 2007 Asian Network for Scientific Information.Öğe Chorological studies on Verbascum ovalifolium subsp. ovalifolium, subsp. thracicum and V. purpureum growing around Edirne(2007) Yilmaz G.; Dane F.In this study, morphological and chorological studies were made on V. ovalifolium Donn ex Sims subsp. ovalifolium, V. ovalifolium Donn ex Sims subsp. thracicum (Velen.) Murb and V. purpureum Hub- Mor. which are all rare taxa for the flora of Turkey. Descriptions of the species and descriptive illustrations scanned with BearPaw1200CUPlus (Mustek) were also given.Öğe Cytological and cytoembryological studies on Paeonia tenuifolia L.(1997) Dane F.The cytology and cytoembryology of Paeonia tenuifolia L. (Paeoniaceae) at Ortakçi Village near Edirne during the years 1989-1991 are considered in this study. Chromosome counts were done on adventive roots, and the results were found to be 2n=10. In the karyotype, 3 median (M), 1 submedian (SM) and 1 subterminal (ST) chromosome pairs were found, in two of which SAT-chromosomes were detected. The anther wall of P. tenuifolia consists of an epidermis , a 1-3 layered endothecium, 1-2 middle layers and a 1-2 layered tapetum. In the secretory tapetum, normal mitosis and the "sticky" type of division were observed. In most microspore mother cells, the course of meiosis is regular. In some cells irregularities were observed, including chromosome bridges, univalent and fragments. Microspore tetrads are isobilateral and tetrahedral, polen grains are tricalporate and are shed at the 2 celled stage. In rare cases, polyspory and multinucleate pollen grains were observed. The course of meiosis in the megaspore mother cell is regular. The ovules are anatrapous, bitegmic and crassinucleate. The female archesporium is multicelled. The female gametophyte formed by the functioning chalazal megaspore is monosporic, of the Polygonum type, in the mature embryo sac, the egg apparatus, the normal appearence of the secondary nucleus and of the antipodal cells. The occurence of double and triple embryo sacs due to the further development of chalazal megaspores of double and triple tetrads was observed.Öğe Cytological and histological studies on reproductive system of hexaploid Bellevalia edirnensis Ozhatay & Mathew (hyacinthaceae).(2006) Dane F.In this study anther wall, meiosis, sporogenesis and the development of female gametophytes of Bellevalia edirnensis were examined cytologically and histologically. The young anther wall consists of an epidermis, a single layered fibrous endothecium, 1-2 middle layers which disappear early, and a single-layered tapetum. In the glandular tapetum; normal mitosis, the "sticky" type of division and secondary nucleus divisions were observed. In most microspore mother cells the course of meiosis is regular. In some cells irregularities were observed including chromosome bridges and univalents. Cytokinesis is of the successive type and the tetrads are isobilateral. The ovules are anatrapous, bitegmic and crassinucellate. The micropyle is formed by the inner integument. The female archesporium is unicelled and directly functions as the megaspore mother cell. The female gametophyte formed by the functioning chalazal megaspore is monosporic, of the Polygonum type.Öğe The effect of waste water on root growth and mitosis in onion (Allium cepa) root apical meristem(Asian Network for Scientific Information, 2006) Dane F.; Ekici N.; Aktaş Y.K.In this study, the phytotoxic and genotoxic effects of waste waters and also mitotic index were investigated in onion (Allium cepa) root tip cells during germination. For this aim, samples of water from effluent channels of Olmuksa-paper, paddy and Textile-thread Factories were used in Edirne-Turkey on March 2002. The mineral content of the effluent was monitored by flame AAS and pH values of samples were measured. Tap water was used as control. The mitotic frequency of the onion root tip meristematic cells increased in samples of water from effluent channels. On the basis of present findings it may concluded that low concentrations of minerals in waters of effluent channels of textile-thread, paddy and Olmuksa-paper mill has some positive effects on root growth and mitotic divisions in onion root tip cells. © 2006 Asian Network for Scientific Information.Öğe The effects of different musical elements on root growth and mitosis in onion (Allium cepa) root apical meristem (musical and biological experimental study)(Asian Network for Scientific Information, 2007) Ekici N.; Dane F.; Mamedova L.; Metin I.; Huseyinov M.In this study effects of strong, complex, rhythmic accent classical music with sekunda and kvarta intervals and frequently reprized and opus with rhythmic dynamically changing lyrics which contain more extensive kvinta septa oktava intervals on mitotic index and root growth were investigated in onion (Allium cepa) root tip cells during germination. For this aim, music samples from Wagner, Mozart, Musorgsky, (Boris Godunov) Chopin, Tchaikovski, Schubert were chosen. We found correlation between root elongation and Mitotic Index (MI). Both kinds of music have positive effects on root growth and mitotic divisions in onion root tip cells but rhythmic dynamically changing lyrics affected much better. In this study light microscopy techniques were used but ultrastructure of root tip cells will be studied with electron microscope in the following study. © 2007 Asian Network for Scientific Information.Öğe Histological Aspects of Anther Wall in Male Fertile and Cytoplasmic Male Sterile Sunflower (Helianthus Annuus L.)(2003) Meric C.; Dane F.; Olgun G.In this study, development of anther wall of Helianthus annuus L. male fertile HA 89 "B" line and cytoplasmic male sterile HA 89 "A" line were compared by light and electron microscopy. It was observed that there was no difference between male fertile HA 89 "B" line and male sterile HA 89 "A" line development of anther wall until tetrads were formed. After tetrad stage it was observed in male fertile HA 89 "B" line that the middle layer was lost, tapetum was parted from anther wall and it surrounded the microspores. In cytoplasmic male sterile HA 89 "A" line, middle layer was vacuolated and widened, tapetum also enlarged and it filled anther sac. It was determined that middle layer and tapetum were permanent with epidermis and endothecial cell during the further development of anther wall, and also there was no secondary thickening in endothecial cells. In conclusion it was found that the plasmodial tapetum which developed in fertile HA 89 "B" line transferred nutrients to microspores and helped them develop normally, but in cytoplasmic male sterile HA 89 "A" line the plasmodial structure did not develop. The inner tangential wall of the tapetum which was related with tetrads was not degenerated, sufficient nutrient were not transferred to microspores and thus they degenerated into the callose wall. © 2003, by Walter de Gruyter Berlin/Boston. All rights reserved.Öğe Karyological studies on Vicia sativa L. subsp. incisa (Bieb.) Arc. var. incisa(1999) Meriç Ç.; Dane F.Vicia sativa L. subsp. incisa (Bieb.) Arc. var. incisa was investigated karyologically (chromosome number, karyotype and mitotic division). The chromosome number of Vicia sativa subsp. incisa var. incisa was counted for the first time and it was found to be 2n=14. In the karyotype, 1 submedian (SM) and 6 subterminal (ST) chromosome pairs were found, in one of which a SAT-chromosome was detected. A satellite was on the short arm of the 3rd chromosome pair. In the cells of the root tips, the course of mitosis was regular. The presence of subsp. incisa var. incisa in the flora of Turkey was also confirmed with the specimens (A1 (E) Edirne: EDTU 6851, EDTU 6852, EDTU 6853, EDTU 7287, ISTE 74462; A2(E) Istanbul: EDTU 7288).Öğe Mitotic changes in root meristems of Lens culinaris treated with fusilade (fluazifop-p-butyl)(2008) Aksoy O.; Ekici N.; Dane F.In this study the genotoxic effects of Fusilade (Fluozifop-p-butyl), a selective herbicide were investigated in the mitotic cell division in lentil (Lens culinaris Medik.) root tip cells. For this aim, different doses (0.25, 5, 1 and 1.5%) of Fusilade solutions were used. Root tips after having grown to a certain length were stained according to aceto orcein squash procedure and the number of abnormal cells was counted in each phase of mitosis. The obtained results indicate that Fusilade had the ability to cause production of a large number of mitotic abnormalities. These abnormalities appeared in varying degrees depending on the dose. The types of abnormalities produced were laggards of the chromosomes, bridges, c-metaphase, stickiness, chromatin granulation and nucleus deformation. According to our findings we can say that Fusilade can produce negative effects on mitotic divisions in lentil root tip cells. © 2008 Academic Journals Inc.Öğe Morphological and physiological effects of herbicide attribut (propoxycarbazone-sodium) and surfactant biopower (sodium alkylether-sulphate) on triticum aestivuml. CV. Pehlivan(2012) Yilmaz G.; Dane F.; Turan F.N.The goals of the study were to investigate morphological and physiological effects of herbicide Attribut (propoxycarbazone-sodium) and surfactant BioPower (sodium alkylether-sulphate) on Triticum aestivum L. cv. Pehlivan. For the experiments, Triticum aestivum were treated with Attribut at 4 different doses of 0.21, 0.42, 0.82 and 1.68 mM.. In order to evaluate the combined effects of BioPower and Attribut, 0.25% BioPower was added in each of applied Attribut doses. The laboratory studies showed that the EC (effective concentration) value corresponded to farmer value of (0.42 mM Attribut). Applications were done either by seed treatments or spreading. The toxic effects of each doses applied on wheat plants were monitored in terms of morphological and physiological parameters. STATISTICA AXA 7.1 statistical program which has number AXA507C775506FAN3 was used for statistical analysis. The results showed that root and shoot lengths during different growth periods of wheat and germination rates decreased, amounts of chlorophyll in leaves decreased while amount of protein in seeds increased with (0.82 mM Attribut), (0.82 mM Attribut + 0.25% BioPower) doses and more of that concentrations were found to be toxic for wheat plants. These toxic effects increased in use with BioPower. © by PSP.Öğe Oxalis europaea Jordan, O. stricta L. and O.corymbosa D.C. (Oxalidaceae): New records for the flora of Turkey(Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade, 2015) Dane F.; Yalçin S.; Yilmaz G.In this study, three new species Oxalis europaea Jordan, O. stricta L. and O. corymbosa D.C. (Oxalidaceae) are recorded for the flora of Turkey with the specimens collected from A1(E) Edirne in European Turkey. Short morphological descriptions including photographs of the species are given. In addition, a new key that contains the new Oxalis species added to the flora of Turkey has been constructed. © 2015 Institute of Botany and Botanical Garden Jevremovac, Belgrade.Öğe Phytotoxic effects of six surfactants on Allium cepa L. plantlets(2012) Akbaş H.; Dane F.; Yilmaz G.; Öztürk F.; Leventer S.In this study, the effects of two anionic surfactants, sodium dodecyl benzene sulfonate (DBSNa) and sodium dodecyl sulfate (SDS), one meric and one dimeric cationic surfactant, cetyltrimethylammonium bromide (CTAB) and gemini surfactant (16-2-16), and two non-ionic surfactants, polyoxyethylene tert-octylphenyl ether (Triton X-100) and polyoxyethylene (23) lauryl ether (Brij 35) were evaluated under laboratory conditions using onions (Allium cepa L.) as test material. The phytotoxic effects of surfactant solutions were observed. The phytotoxic effects on roots were determined by measuring root elongations of onions for each day in a week under laboratory conditions at 16.0±0.5°C, after treated them by different surfactants. All surfactants used in this study were tested at three different concentrations, 5.00, 2.50 and 1.25 g/L (w/v), and some differences were observed between the effects of them. The phytotoxic effects were observed on onions which treated by anionic, cationic surfactants and nonionic surfactant Triton X-100. Stimulatory effects only occurred on onions treated by Brij 35 while inhibitory effects were observed on onions treated by all concentrations of Triton X-100. It was observed that inhibitory effects increased with increasing concentrations of Triton X-100. © by PSP.Öğe Phytotoxicity induced by herbicide and surfactant on stomata and epicuticular wax of wheat(2012) Yilmaz G.; Dane F.This study was performed in order to determine some of toxic effects of different doses of Attribut (propoxycarbazone-sodium) used in wheat fields and of BioPower (sodium alkylether-sulphate) surfactant added Attribut on epicuticular wax deposition and stomata on Triticum aestivum L. cv Pehlivan by scanning electron microscope. The doses used in the study were (0.42 mM Attribut) (Farmer Dose) (FD), (0.82 mM Attribut) (FD × 2), (1.68 mM Attribut) (FD×4) and the same doses including 0.25% of BioPower surfactant. Applications were done by seed and leaf treatments under field conditions. In SEM studies, in (FD × 2), (FD × 4), (FDS × 2) and (FDS × 4) applications, plasmolysis of the epiderma and morphological changes in the cuticle, unlike the control, were determined. It was observed in (FD × 2) and (FD × 4) applications that volumes of the stomata narrowed and they went into plasmolysis. In (FDS × 2) and (FDS × 4) applications, especially reduction in wax and alterations in wax crystals were determined.Öğe Pollen morphology of exotic trees and shrubs of Edirne II(2012) Tütüncü Konyar S.; Dane F.This study was undertaken to examine the pollen morphology of some of the exotic shrubs and trees found in the parks and the gardens of Edirne. In the present work five plant species were studied in this respect, namely: Aesculus hippocastanum, Buddleia davidii, Crataegus oxycantha, Elaeagnus angustifolia and Philadelphus coronarius. Pollen samples belonging to these species were examined with light photomicroscope as regards some morphological features, principally: pollen shape; size; aperture characters; sculpture and structure of the exine. Pollen grains of examined species were spheroidal in shape and most often tricolporate type. Exine layer of examined grains exhibited eutectate structure except the exine of the Philadelphus coronarius which exhibited semitectate structure. Exine ornemantation (Sculpture) of pollen grains had determined as striate in Aesculus hippocastanum, Rugulate-Perforate in Buddleia davidii, Striate - Perforate in Crataegus oxycantha, Psilate-verrucate in Elaeagnus angustifolia and reticulate in Philadelphus coronarius.Öğe Pollen tube growth of Paeonia tenuifolia L. (Paeoniaceae) in vitro and in vivo(Bangladesh Botanical Society, 2011) Dane F.; Ekici N.In vitro and in vivo studies on pollen germination of Paeonia tenuifolia L. (Paeoniaceae) revealed that pollen grains are shed at two-celled stage. Normal and abnormal pollens were observed. Pollen viability was recorded between 55 and 75%. In vitro studies revealed 85% germination and usually the germination was monosphonic. Some pollen tubes with swollen tube tip and undulations were found. Styles and stigma were found to Contain many pollen tubes 24 hrs after pollination.Öğe Some ultrastructural observations on calcium oxalate raphide crystal idioblasts and meristematic cells of the adventive root tips of Sternbergia lutea (L.) Ker-Gawl. ex Sprengel (Amaryllidaceae)(2000) Dane F.; Meriç C.; Hüseyinova G.The adventive root tip cells of a bulbous plant. Sternbergia lutea (L.) Ker-Gawl. ex Sprengel (Amaryllidaceae), were examined by LM (light microscope) and EM (electron microscope). At the meristematic area of the root tips, which was 1 mm in length, two types of cell were observed: meristematic cells and Ca-oxalate raphide crystal idioblasts. Ca-oxalate raphide crystal idioblasts were observed to lie in two lines between the inner and outer ground meristems. Some similarities and some differences were found between those cells in their ultrastructure. It was seen that the membrane thickness of crystal idioblasts and meristematic cells were the same. They were also found to be similar in cell content, both containing nuclei, cytoplasm, vacuoles, mitochondria and proplastids. However the organization of the cell contents, the numbers, the forms and sizes of vacuoles were different between these cell types. In some meristematic cells and idioblasts of certain roots, some degenerated changes were seen due to necrosis. Those changes were in the form of plasmolysis. karyolysis, karyorrhexis. chromatin roughness, karyopyknosis and organelle loss.Öğe Verbascum samniticum Ten. (Scrophulariaceae): A new record for the flora of Turkey(2008) Yilmaz G.; Dane F.Verbascum samniticum Ten. (Scrophulariaceae) is recorded for the first time for the flora of Turkey with the specimen collected from A1 (E) Kirklareli. A description of the species and descriptive illustrations are given based on the collected specimen. Its relationship with V. phlomoides L. is discussed. © TÜBİTAK.
