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Öğe Anemone cinsinin Trakya'da rastlanan türlerinin sistematik, dış morfoloji ve yayılışları üzerinde araştırmalar(Trakya Üniversitesi, 1987) Dane, Feruzan; Demiriz, HüsnüÖZET 1986 yılı ilkbaharında A 1(E) karesi içinde kalan? Edirne, Kırklareli, Tekirdağ ve Çanakkale çevresinden toplanan taze ve 'kuru herbaryum materyali üzerinde sistematik ve morfolojik araştırmalar yapıldı. Trakya'da varlığı bilinen Anemone L. türlerinden: 1, A.nemorosa L. 2.- A.ranunculoides L. subsp. ranunouloides. 3. A.blanda Schott et Kotsehy. 4. A. coronaria L. 5. A. pavonina Lam. üzerinde çalışıldı. Araştırma sırasında bu türlere ilave olarak A. pavonina Lam. türünün yeni bir varyetesi (A.pavonina Lam. var. alba var. nov. ) bulundu. Türlerin adları sinonimleriyle birlikte verildi. Sistematik ve morfolojik özellikleri alman ölçüm sonuçları da değerlendirilerek belirtildi. Toplanan materyalin yetiştiği ortamın ekolojik özellikleri arazi çalışmaları sırasında gözlendi. Trakya'da yayılış alanları ile ilgili açıklamalar yapıldı. Örneklerin toplandığı alanlar haritada işaretlendi. şekiller floralarda yayınlanan ikonografyalara uygun şekil de düzenlendi ve sunuldu.Öğe Anther ontogeny in Campsis radicans (L.) Seem. (Bignoniaceae)(Springer Wien, 2013) Konyar, Sevil Tutuncu; Dane, FeruzanIn this study anther ontogeny of Campsis radicans (L.) Seem. was investigated by transmission electron microscopy and light microscopy with special reference to the development of the anther wall. The anther wall formation follows the dicotyledonous type. The differentiation in anther starts with the appearance of archesporial cells which undergo periclinal divisions to give primary parietal layer to the epidermal site and the primary sporogenous cells to the inside. The primary parietal layer also divides to form two secondary parietal layers. Later, the outer secondary parietal layer (spl1) forms the endothecium and the middle layer by periclinal division whereas the inner one (spl2) directly develops into the outer tapetum forming the inner most layer of the anther wall. The sporogenous tissue is generally organized in two rows of cells with a horseshoe-shaped outline. The remainder of the tapetum lining the sporogenous mass is derived from the connective tissue. The tapetum thus has dual origin and dimorphic. Anthers are tetrasporangiate. The wall of the anther consists of an epidermis, endothecium, middle layer, and the secretory type tapetum. Tapetal cells are usually binucleated. Epidermis and Endothecium layers of anther wall remain intact until the end of anther and pollen development; however, middle layer and tapetum disappear during development.Öğe Calcium oxalate crystals in generative organs of Astragalus hamosus and Astragalus glycyphyllos(2013) Yılmaz, Gülden; Aksoy, Özlem; Dane, FeruzanBu çalışmada, Astragalus hamosus L. and Astragalus glycyphyllos L. un generatif organlarındaki kalsiyum okzalat kristal tiplerinin dağılımı ışık mikroskobu kullanılarak incelenmiştir. A. glycyphyllos taki sitoembryolojik çalışmalar boyunca generatif organlarda CaOx kristalleri gözlenmiştir. A. hamosus ta gözlenmediği halde A. glycyphyllos un çiçeklerinin farklı bölgelerinde prizmatik ve druz tip kristaller gözlenmiştir. Bu çalışma incelenen Astragalus türlerinde CaOx kristallerinin varlığını göstermektedir.Öğe A COMPARATIVE STUDY ON THE SIGNIFICANCE OF LEAD, NICKEL AND CADMIUM ON ROOT GROWTH, THE KINETICS OF METAL ION TRANSPORT AND pH CHANGES IN ONION (ALLIUM CEPA) ROOT(Parlar Scientific Publications (P S P), 2016) Akbas, Halide; Dane, Feruzan; Meric, CilerIn this study, the effect of different concentrations of lead (0.25, 0.50, 1.00 and 2.00 mM) uptake by roots, on root growth of Allium cepa, and the transport of lead ion (Pb2+), iron ion (Fe2+), manganese ion (Mn2+) and hydrogen ion (H+) were investigated spectrophotometrically. The uptake of Pb2+, Fe2+ and Mn2+ by Allium cepa were monitored by a Unicam model 929 flame atomic absorption spectrophotometer (AAS) and pH values were measured by a Jenway 3040 Ion Analyzer with a combination glass electrode. The mineral content of the solution was measured instead of the mineral content of the root material. Lead ions had inhibitory effect on the root growth in all concentrations during the entire treatment. The EC50 was found at 0.50 mM Pb2+. A large amount of Pb2+ was translocated into A. cepa roots. The kinetics of metal ion transport was described by a pseudo-first order reaction at 1.00 and 2.00 mM metal ion concentrations. Pb2+, Fe2+ and H+ ions transfer into plant from solution but Mn2+ ion transfer to the solution from the plant. The amount of H+ in the solution is increased at all Pb+2 concentrations except 0.25 mM Pb+2 concentration. The results of this study are compared with previous studies with nickel and cadmium treatment.Öğe Cytochemistry of pollen development in Campsis radicans (L.) Seem. (Bignoniaceae)(Springer Wien, 2013) Konyar, Sevil Tutuncu; Dane, FeruzanIn this study, cytochemical staining methods were used to follow the cytochemical modifications of microspore cytoplasm and sporoderm in Campsis radicans (L.) Seem. from tetrad stage to mature pollen. Flower buds were collected at different stages of development, and the anthers were fixed and embedded in Araldite. To make cytochemical observations under light microscope, semithin sections were cut and stained with different dyes. Cytochemical methods provided the opportunity to localize the reserve material in the microspore and pollen cytoplasm, to distinguish the different layers of the sporoderm, and to determine its chemical structure at different developmental stages. Microspore cytoplasm contains variable amounts of proteins, lipids, and insoluble carbohydrates at different stages of microsporogenesis. Sporoderm formation starts at tetrad stage by the formation of primexine and is completed at vacuolated microspore stage by the addition of sporopollenin from tapetum. During the vacuolization and enlargement of the microspores, the structure and the chemical composition of the exine are modified. The endexine becomes chemically different from the ectexine. The ectexine is composed of sporopollenin and a small amount of protein, whereas the endexine is composed of sporopollenin, proteins, and traces of polysaccharides.Öğe Cytological and histological studies on female gametophyte of Leucojum aestivum (Amaryllidaceae)(Springer, 2008) Ekici, Nuran; Dane, FeruzanIn this study, gynoeceum, development of megasporangium, megasporogenesis, megagametogenesis and female gametophyte of Leucojum aestivum were examined cytologically and histologically. Ovules of L. aestivum are of anatropous, bitegmic and crassinucellate type. Inner integument forms the micropyle. Archesporial cell develops directly into a megasporocyte. Embryo sac development is of bisporic Allium type. Filiform apparatus is observed in synergids. Polar nuclei fuse before fertilization to form secondary nucleus near the antipodals.Öğe Cytological and histological studies on reproductive system of hexaploid Bellevalia edirnensis Ozhatay & Mathew (Hyacinthaceae)(Akademiai Kiado, 2006) Dane, FeruzanIn this study anther wall, meiosis, sporogenesis and the development of female gametophytes of Bellevalia edirnensis were examined cytologically and histologically. The young anther wall consists of an epidermis, a single layered fibrous endothecium, 1-2 middle layers which disappear early, and a single-layered tapetum. In the glandular tapetum; normal mitosis, the sticky type of division and secondary nucleus divisions were observed. In most microspore mother cells the course of meiosis is regular. In some cells irregularities were observed including chromosome bridges and univalents. Cytokinesis is of the successive type and the tetrads are isobilateral. The ovules are anatrapous, bitegmic and crass-inucellate. The micropyle is formed by the inner integument. The female archesporium is unicelled and directly functions as the megaspore mother cell. The female gametophyte formed by the functioning chalazal megaspore is monosporic, of the Polygonum type.Öğe Demonstration of the effects of Raxil (tebuconazole) on the cell wall (glycocalyx) of the root tip cells in onion (Allium cepa L.) using Alcian blue(Parlar Scientific Publications (P S P), 2007) Aktac, Tulin; Yilmaz, Gulden; Dane, Feruzan; Bakar, ElvanIn this study, the effect of Raxil FS 060 (60 g/L tebuconazole) on the cell wall of root tip cells of Allium cepa was investigated using the Alcian blue-specific interaction with glycosaminoglycans (GAGs). The bulbs with roots of A. cepa were treated with different concentrations of Raxil for two days. Germinated root tips of onions were stained with 1% Alcian blue 8 GX, and counter-stained with 2% aceto orcein solution for 5 min (acetic orcein squash method). In the root tips treated with different concentrations of Raxil (0.8%, 6.6% and 26.4% doses), it could be shown that the cell walls were thickened by increasing doses. Constrictions of protoplasm and dissolutions of nuclei in root tip cells were clearly observed. According to these results, it was concluded that the triazole fungicide, tebucanozole, might be toxic on the root tip cells of Allium cepa.Öğe Distribution of insoluble polysaccharides, neutral lipids, and proteins in the developing anthers of Campsis radicans (L.) Seem. (Bignoniaceae)(Springer Wien, 2013) Konyar, Sevil Tutuncu; Dane, Feruzan; Tutuncu, SerpilIn this study, distribution of polysaccharides, lipids, and proteins in the developing anthers of Campsis radicans (L.) Seem. was examined from sporogenous cell stage to mature pollen, using cytochemical methods. To detect the distribution and dynamic changes of insoluble polysaccharides, lipid bodies, and proteins in the anthers through progressive developmental stages, semi-thin sections of anthers at different developmental stages were stained with periodic-acid-Schiff (PAS) reagent, Sudan black B, and Coomassie brilliant blue, respectively, and examined under light microscope. Ultrastructural observations with TEM were also carried out to determine the storage form of starch in the connective tissue, and storage form of lipids in the tapetal cells. In sporogenous cell stage, anther wall contains numerous insoluble polysaccharides. However, from the sporogenous cell stage to the vacuolated microspore stage, the amount of insoluble polysaccharides in the anther wall decreases gradually. At bicellular pollen stage, tapetum degenerates completely and polysaccharides are not seen in the anther wall. Lipid bodies are observed in the cytoplasm of both middle layer and tapetal cells at tetrad stage, whereas they disappear in the vacuolated microspore stage. Compared with polysaccharides, proteins are limited in the anther wall at early stages of development. During pollen development, polysaccharides, proteins, and lipid bodies are scarce in the cytoplasm of sporogenous cells, but their amount increases at premeiotic stage. From tetrad stage to bicellular pollen stage, microspore cytoplasm contains variable amount of insoluble polysaccharide grains, lipid and protein bodies. At bicellular pollen stage, plentiful amount of starch granules are stored in the cytoplasm of the pollen grains. Proteins and lipid bodies are also present in the cytoplasm.Öğe Effect of nickel on root growth and the kinetics of metal ions transport in onion (Allium cepa) root(Natl Inst Science Communication-Niscair, 2009) Akbas, Halide; Dane, Feruzan; Meric, CilerThe effect of different concentrations of nickel nitrate (0.25, 0.50, 1.00 and 2.00 mM) uptake by the roots, on root growth of onion (Allium cepa) and the transport of Ni2+, Fe2+, Mn2+, Zn2+, K+, Na+ and H+ ions were investigated spectrophotometrically. The uptake of Ni2+, Fe2+, Mn2+ and Zn2+ was monitored by flame atomic absorption spectrometry with a 24-h period for 7 days and the amounts of K+ and Na+ were determined in solutions by flame photometer, The mineral content of the solution, instead of the root material was measured. Ni2+ ions showed inhibitory effect oil the root growth at all concentrations during the entire treatment. The EC50 (effective concentration that reduced root growth by 50%) was found at 0.25 mM Ni2+. No significant change in inhibitory effect was observed after at 0.50 mM Ni2+ concentrations. A large amount of Ni2+ was translocated into the roots. The kinetics of metal ion transport followed a pseudo-first order reaction in all metal ion concentrations. Ni2+, Zn2+ Fe2+ Mn2+ and H+ ions transferred together into plant, but Na+ and K+ ions transferred to the solution from the plant. The amount of H+ in the solution decreased at all Ni2+ concentrations.Öğe The effects of Fusilade (Fluazifop-p-butyl) on germination, mitotic frequency and ?-amylase activity of lentil (Lens culinaris Medik.) seeds(Springer, 2007) Aksoy, Ozlem (Dalgic); Dane, Feruzan; Sanal, Filiz Ekinci; Aktac, TulinIn this study, seed germination percentages, effects on phases of mitosis and alpha-amylase enzyme activity of lentil seeds treated with four different concentrations (0.25, 0.5, 1 and 1.5%) of Fusilade (Fluazifop-p-butyl) were determined. Median EC ( effective concentration) values were calculated according to seed germination percentages after treatment for 72 h. Germination percentages of primary lentil roots decreased with increasing Fusilade concentrations. Cytological observations showed that the mitotic frequency in root meristematic cells were decreased parallel to the increase in concentrations and all Fusilade concentrations applied decreased the activity of a-amylase enzyme in lentil seeds. The obtained results indicate that the herbicide Fusilade had the ability to cause reduction in seed germination, mitotic frequency and also alpha-amylase activity of lentil seeds.Öğe The effects of Fusilade (Fluazifop-p-butyl) on root and shoot growth of lentil (Lens culinaris medik.)Seedlings(2007) Aksoy, Dalgic Özlem; Dane, FeruzanThe present study has been carried out to investigate the effects of the herbicide Fusilade (Fluazifop-p-butyl) on root and shoot growth of lentil seedlings. Four different doses of Fusilade (0.25%, 0.5%, 1% and 1.5%) were used in (Fluazifop-p-butyl) Lens culinaris seeds and leaves. The obtained results indicate that shoot growth and lateral root growth was reduced in Fusilade treated groups. Leaf deformations like chlorosis, curling, expansion and asymmetry was observed in Fusilade treated leaves. It was also seen that leaf treatment was more sensitive than seed treatment against the effects of herbicide Fusilade.Öğe Examination of pollen morphology of some exotic trees and shrubs found in the parks and the gardens of Edirne (European Turkey) I.(2007) Dane, Feruzan; Tütüncü, Sevil; Tütüncü, SerpilThis study was undertaken to identify some exotic shrubs and trees found in the parks and the gardens in Edirne and to examine morphology of their pollen. In the present work five plant species were studied in this respect, namely: Tamarix parviflora (Tamaricaceae), Campsis radicans (Bignoniaceae), Prunus serrulata (Rosaceae), Laburnum anagyroides (Fabaceae) and Lagerstroemia indica (Lytraceae). Pollen grains belonging to these species were examined with Olympus BH-2 light photomicroscope as regards some morphological features, principally: pollen shape; size; aperture characters; sculpture and structure of the exine.Öğe Hekzaploid (2n=24) Bellevalia edirnensis Özhatay & Mathew’in polen mitozu ve polen morfolojisinin incelenmesi(1999) Dane, FeruzanHekzaploid (2n=24) Bellevalia edirnensis Özhatay & Mathew’in polen mitozu ve polen morfolojisi incelendi. Mikrosporların büyüklük bakımından varyasyon gösterdikleri, ekzin ve apertürün polen mitozu baslamadan önce gelistigi görüldü. Polen mitozunda polarite, asimetrik ig ve normal mitoz bölünmelerle birlikte bazı anormalliklerde gözlendi. Polaritede hem nukleus göçünün hemde vakuol olusumunun rol oynadıgı saptandı. Vegetatif hücrenin distal kutupta, generatif hücrenin ise proksimal kutupta yer aldıgı görüldü. Olgun polenlerin iki hücreli, polen seklinin sphaeroidea, polen tipinin trichotomosulcatae, ekzin strüktürünün (yapısının) tectatae, skulpturun (ekzin süslerinin) ise reticulatae oldugu belirlendi. B. edirnensis’in incelenen bu özellikleri ilk defa bu çalısmada saptandı.Öğe In situ germination of pollen tetrads in Periploca graceae L. (Periplocaeae)(2000) Dane, FeruzanPeriploca graceae L. (Periplocaceae) bitkisinin büyük çiçek tomurcuklarındaki olgun anterler sitolojik olarak incelendi. Polenlerin tetradlar seklinde bulundugu görüldü. Polen tertradlarının genellikle isobilateral nadiren linear seklinde oldugu gözlendi. Ayrıca polen tetradlarının anter lokusunda çimlendikleri görüldü. Tetrad polenlerinin iyi boyandıgı ve çimlenme yüzdesinin 90 % gibi oldugu saptandı. In situ çimlenen polen tetradlarında olusan tüp sayısının 1-4 arasında degistigi gözlendi. Polen tetradlarının çimlenmesi sırasında nukleusların normal davranıs gösterdikleri saptandı. Polen tüpü olusumunun erken fazında tüpte vegetatif nukleus ve 2 sperm hücresi görüldü. Polen tüplerinde normal gelisimler gözlendigi gibi bazı anormallikler de gözlendi. Bu anormalliklerin hücre çeperinde kalınlasma ve kalloz çeperde artma ile tüp ucu çatallanması seklinde oldugu saptandı.Öğe In vitro pollen germination of some plant species in basic culture medium(2004) Olgun, Göksel; Dane, Feruzan; Dalgıç, ÖzlemBu çalışmada Malva sylvestris L. (Malvaceae), Ecballium elaterium (L.) A. Rich. (Cucurbitaceae), Lonicera fragrantissima Lindley (Caprifoliaceae), Cyclamen hederifolium Aiton (Primulaceae), Linaria vulgaris Miller (Scrophulariaceae), Antirrhinum majus L. (Scrophulariaceae), Yucca filamentosa L. (Liliaceae)'ye ait toplam 7 bitki türünün polenlerine temel kültür ortamında in vitro koşullarda çimlenme testi uygulanmıştır. Çimlenme ortamı olarak %10'luk sakkaroz ve %0,01'lik borik asit kullanılarak, petrilerde çimlendirilmiştir. Türler arasında in vitro çimlenme yüzdeleri oldukça farklı bulunmuştur. Çimlenme yüzdeleri; Anthirrinum majus'ta. %80, Linaria vulgaris 'de %70, Cyclamen hederifolium 'da %30, Lonicera fragrantissima'da %15 ve Ecballium elaterium'da. %5 olarak bulunmuştur. Malva sylvestris ve Yucca filamentosa'nm polenlerinde ise çimlenme gözlenmemiştir.Öğe Micellization and Related Behaviours of Polyoxyethylene-Type Non-ionic Surfactants on Root Elongation of Allium cepa L.(Asian Journal Of Chemistry, 2012) Yilmaz, Gulden; Akbas, Halide; Dane, Feruzan; Leventer, Sinem; Ozturk, FatmanurIn this study, polyoxyethylenated fatty alcohol ether (POE) nonionic surfactants polyoxyethylene (23) lauryl ether (C12E23) (Brij 35); polyoxyethylene (10) cetyl ether (C16E10) (Brij 56); polyoxyethylene (10) stearyl ether (C18E10) (Brij 76); polyoxyethylene (10) lauryl ether (C12E10) (POE 10). A large proportion nonionic surfactants are made hydrophilic by the presence of a polyethylene glycol chain obtained by the polycondensation of ethylene oxide. They are called polyethoxylated nonionics. In this study the effects of polyoxyethylene-type nonionic surfactants on root elongation of Allium cepa L. were determined for a week under laboratory conditions in the concentration ranges of 5.00, 2.50 and 1.25 g/L (w/v) at 16 +/- 0.5 degrees C. It was determined that the stimulatory effect on root elongation becomes stronger when the number of ethylene oxide group (C2H4O) was increased in the POE surfactant. The stimulatory effect decreases in the order C18E10 > C16E10 > C12E23 > C12E10 due to the decreasing of the number hydrocarbons in the series.Öğe Morphological and anatomical contributions to the taxonomical identification of two Ornithogalum taxa (O. nutans and O. boucheanum) from Flora of Turkey(Springer, 2011) Meric, Ciler; Aksoy, Ozlem; Dane, FeruzanIn this study, morphological and anatomical features of Ornithogalum nutans and O. boucheanum, two relative and morphologically similar species growing in European Turkey, were investigated. These species showed some important anatomical differences with regard to leaf anatomy while they have identical features in stem. The stem anatomy of these two species displays the common properties of monocotyledons. The mesophyll is unifacial and contains monotypic chlorenchyma cells in the leaf of O. nutans. It has no lacunae. The mesophyll in O. boucheanum is equifacial and it has lacunae. This anatomical distinction may be useful for the identification of these similar-looking species.Öğe MORPHOLOGICAL AND PHYSIOLOGICAL EFFECTS OF HERBICIDE ATTRIBUT (PROPOXYCARBAZONE-SODIUM) AND SURFACTANT BIOPOWER (SODIUM ALKYLETHER-SULPHATE) ON TRITICUM AESTIVUM L. CV. PEHLIVAN(Parlar Scientific Publications (P S P), 2012) Yilmaz, Gulden; Dane, Feruzan; Turan, Fatma NesrinThe goals of the study were to investigate morphological and physiological effects of herbicide Attribut (propoxycarbazone-sodium) and surfactant BioPower (sodium alkylether-sulphate) on Triticum aestivum L. cv. Pehlivan. For the experiments, Triticum aestivum were treated with Attribut at 4 different doses of 0.21, 0.42, 0.82 and 1.68 mM.. In order to evaluate the combined effects of BioPower and Attribut, 0.25% BioPower was added in each of applied Attribut doses. The laboratory studies showed that the EC (effective concentration) value corresponded to farmer value of (0.42 mM Attribut). Applications were done either by seed treatments or spreading. The toxic effects of each doses applied on wheat plants were monitored in terms of morphological and physiological parameters. STATISTICA AXA 7.1 statistical program which has number AXA507C775506FAN3 was used for statistical analysis. The results showed that root and shoot lengths during different growth periods of wheat and germination rates decreased, amounts of chlorophyll in leaves decreased while amount of protein in seeds increased with (0.82 mM Attribut), (0.82 mM Attribut + 0.25% BioPower) doses and more of that concentrations were found to be toxic for wheat plants. These toxic effects increased in use with BioPower.Öğe Occurrence, types and distribution of calcium oxalate crystals in leaves and stems of some species of poisonous plants(Springeropen, 2014) Konyar, Sevil Tutuncu; Ozturk, Necla; Dane, FeruzanBackground: Calcium oxalate crystals, which are found in many organs of plants, have different morphological forms: as druses, prism, styloids, raphides and crystal sand. In this study, the distribution, type and specific location of calcium oxalate crystals in the leaves and stems of the eight species of poisonous plants and one species of nonpoisonous plant were investigated with light microscopy. During study special attention was given to the possible correlation between the presence and types of calcium oxalate crystals and toxic plant organs. The plants examined in this study were Hedera helix L. (Araliaceae), Aristolochia clematitis L. (Aristolochiaceae), Humulus lupulus L. (Cannabaceae), Saponaria officinalis L. (Caryophyllaceae), Chelidonium majus L. (Papaveraceae), Hypericum perforatum L. (Hypericaceae), Tribulus terrestris L. (Zygophyllaceae), Cynanchum acutum L. (Asclepiadaceae), and Nerium oleander L. (Apocynaceae). Results: Three types of crystals: druses, prismatic crystals and crystal sands were observed. Druses were identified in the leaves and stems of six species of studied plants. In contrast to druses, crystal sands and prismatic crystals were rare. Prismatic crystals were observed in the leaf mesophlly cells of both Nerium oleander and Cynanchum acutum. However, crystal sands were observed only in the pith tissue of Humulus lupulus. On the other hand, leaves and stems of Chelidonium majus, Aristolochia clematitis and Hypericum perforatum were devoid of crystals. Conclusion: There is no absolute correlation between the presence and type of calcium oxalate crystals and toxic plant organs. However druse crystals may function as main irritant in toxic organs of the plants.
