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Öğe Effects of caffeic acid phenethyl ester (CAPE) on membrane potential and intracellular calcium in human endothelial cells(Verduci Publisher, 2013) Burgazli, M. Kamil; Aydogdu, N.; Rafiq, A.; Mericliler, M.; Chasan, R.; Erdogan, A.BACKGROUNG AND OBJECTIVES: Caffeic acid phenethyl ester (CAPE) is an active component of the resin propolis obtained from beehives. Propolis has a long history of medicinal use and a number of studies have already reported on some of its pharmaceutical properties. This study aimed to explore the effects of CAPE on the cytosolic Ca2+ concentration, cell proliferation, membrane potential and NO levels in human endothelial cells. MATERIALS AND METHODS: Isolated human umbilical vein endothelial cells (HUVEC) were incubated with CAPE (1-100 mu M) at 37 degrees C for 48 hours. Cell proliferation was estimated by counting cell numbers with use of a Neubauer chamber. The effect of CAPE (1-100 mu M) on the membrane potential was measured with the fluorescence dye DIBAC4(3) whereas its effect on the cytosolic Ca2+ concentration was measured by use of the fluorescence probe Fluo-3 AM (Invitrogen, Leiden, Netherlands). NO production was assayed using the flourophore DAF similar to AM (Invitrogen, Leiden, Netherlands). Changes in fluorescence intensity was determined with the GENios plate reader (Genios, Tecan, Austria). RESULTS: A Dose-dependent hyperpolarization of the endothelial cell membrane was observed with CAPE stimulation. The initial increase in the intracellular Ca2+ concentration showed a subsequent decrease over time. CAPE stimulation also resulted in an increase in NO production; however, at higher doses a decrease in NO levels was observed. HUVEC proliferation was inhibited by CAPE. CONCLUSIONS: Here we report on the effect of CAPE stimulation on the cytosolic Ca2+ concentration, cell proliferation, membrane potential and NO production in HUVEC in a dose-dependent manner. These findings provide important insights into some potential key roles that both calcium and the membrane potential play in the CAPE activation of endothelial cells in a concentration-dependent manner.Öğe The effects of relaxin on myoglobinuric acute kidney injury in rats(Wiley, 2017) Tarhan, A. Ilhan; Aydogdu, N.; Tastekin, E.; Sut, N.[Abstract Not Available]Öğe The effects of Tarantula cubensis extract on renal ischemia/reperfusion injury in the rats(Wiley-Blackwell, 2014) Aydogdu, N.; Tastekin, E.; Cukur, Z.; Poyraz, M. D.; Yavuz, O. Y.; Kaya, O.[Abstract Not Available]Öğe Histopathological and biochemical comparisons of the protective effects of amifostine and L-carnitine against radiation-induced acute testicular toxicity in rats(Wiley, 2017) Aktoz, T.; Caloglu, M.; Yurut-Caloglu, V.; Yalcin, O.; Aydogdu, N.; Nurlu, D.; Arda, E.The aim of this study was to compare the radioprotective efficacies of amifostine (AMI) and L-carnitine (LC) against radiation-induced acute testicular damage. Thirty Wistar albino rats were randomly assigned to four groups: control (n = 6), AMI plus radiotherapy (RT) (n = 8), LC plus RT (n = 8) and RT group (n = 8). The rats were irradiated with a single dose of 20 Gy to the scrotal field. LC (300 mg/kg) and AMI (200 mg/kg) were given intraperitoneally 30 min before irradiation. The mean seminiferous tubule diameters (MSTDs) were calculated. Testicular damage was evaluated histopathologically using Johnsen's mean testicular biopsy score criteria. Malondialdehyde (MDA) and glutathione levels were measured in tissue samples. AMI plus RT and LC plus RT groups had significantly higher MSTDs than those in the RT group (p =.003 and p =.032 respectively). MDA values of both AMI plus RT and LC plus RT groups were significantly lower than those in RT group (p <.004 and p <.012 respectively). As a result, AMI and LC have a similar radioprotective effect against radiation-induced acute testicular damage, histopathologically and biochemically.Öğe Investigation of the possible role of luteolin in breast cancer(Wiley-Blackwell, 2016) Erbas, H.; Kandemir, N.; Dibirdik, I.; Aydogdu, N.; Cakir, E.[Abstract Not Available]Öğe The protective effect of L-carnitine on ionizing radiation-induced free oxygen radicals(Scandinavian Soc Laboratory Animal Science, 2006) Dokmeci, D.; Akpolat, M.; Aydogdu, N.; Uzal, C.; Doganay, L.; Turan, F. N.Ionizing radiation is known to generate reactive oxygen species (ROS) that can be removed by antioxidants. L-carnitine, a natural component of mammalian tissue, is a necessary factor in the utilization of long-chain fatty acids to produce energy. Furthermore it has been shown that L-carnitine is an antioxidant which has a scavenger effect on ROS and a stabilizing effect on damaged cell membranes. The aim of the study was to evaluate the potential protective effect of L-carnitine on radiation-induced free radicals in hamsters. L-carnitine was given by gavage at a dose of 50 mg/kg for 15 consecutive days before irradiation with a single dose of 8 Gy. 24 h after radiation exposure, the hamsters were sacrificed and samples were taken from blood and tissues, and the biochemical and histopatological determinations were carried out. In the irradiated group, there were significant increases in plasma and liver malondialdehyde (MDA) with marked reduction in glutathione (GSH) levels in the liver, compared with controls. In red blood cells, superoxide dismutase (SOD) and catalase activities were also reduced. All these effects were reversed by L-carnitine. In conclusion, L-carnitine with its antioxidant and free radical scavenging properties could play a modulatory role against the cellular damage produced by free radicals induced by ionizing radiation.Öğe PROTECTIVE EFFECTS OF AMIFOSTINE AND L-CARNITINE AGAINST RADIATION INDUCED ACUTE TESTICULAR TOXICITY(Elsevier Ireland Ltd, 2012) Caloglu, M.; Aktoz, T.; Caloglu, V. Yurut; Yalcin, O.; Aydogdu, N.; Nurlu, D.; Arda, E.[Abstract Not Available]Öğe The role of kisspeptin in myoglobinuric acute kidney injury in rats(Wiley, 2019) Iskan, N. G.; Soyleyici, B.; Kandemir, N.; Aydin, Ali M.; Aydogdu, N.[Abstract Not Available]
