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    Antidiabetic activity evaluation of Onobrychis species on alloxan-induced diabetic mice
    (Univ Sao Paulo, Conjunto Quimicas, 2019) Ozbek, Hanefi; Acikara, Ozlem Bahadir; Oz, Burcin Ergene; Ozbilgin, Serkan; Kirmizir, Neriman Ipek; Ozrenk, Bade Cevriye; Tekin, Mehmet
    In the current study, four Onobrychis species, O. albiflora Hub.-Mor., O. argyrea Boiss. subsp. argyrea Boiss., O. galegifolia Boiss., and O. tournefortii (Willd.) Desv. were collected from Anatolia to be evaluated for their antidiabetic activities. Methanol water extracts of the aerial parts were used for experiments. An alloxan-induced diabetic mice test model was used. Phytochemical analysis of the tested extracts was investigated using the HPLC method. The highest activity was observed with treatment of O. albiflora aerial part extract. Significant decrements were detected in the blood glucose levels as follows: 180.83 +/- 47.48 and 252.83 +/- 50.47mg/dL at 100 mg/kg and 200 mg/kg doses of O. albiflora, respectively, when compared to the isotonic saline solution control group, eliciting a blood glucose level of 494.20 +/- 27.32. Among the tested standard compounds, rutin and isoquercetin were detected in the examined species. The highest amount of rutin (1.1981 +/- 0.0017%) and isoquercetin (0.7318 +/- 0.0197%) were found in O. albiflora and O. argyrea subsp. argyrea, respectively. Antidiabetic activities of the tested Onobrychis species seem to indicate a possible correlation with their rutin and isoquercetin contents. Therefore, rutin and isoquercetin may be the antidiabetic compounds that contribute to the antidiabetic activity of the tested Onobrychis species.
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    Bioactivities of Alchemilla mollis, Alchemilla persica and Their Active Constituents
    (Univ Sao Paulo, Conjunto Quimicas, 2022) Kurtul, Ekin; Eryilmaz, Mujde; Sarialtin, Sezen Yilmaz; Tekin, Mehmet; Acikara, Ozlem Bahadir; Coban, Tulay
    The aim of this study was to determine antimicrobial activities of Alchemilla mollis, Alchemilla persica as well as ellagic acid and miquelianin against Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans by using microbroth dilution method and anti-inflammatory activity by using human red blood cell (HRBC) membrane stabilization method. Microbroth dilution method was used to determine the antimicrobial activities. Extracts possessed activity having MIC values of 2.5-5-10mg/mL, compounds possessed activity having MIC values of 1.25-2.5- 4-5mg/mL. A.mollis aerial parts displayed the highest anti-inflammatory activity (IC50=1.22 +/- 0.07mg/mL). Ellagic acid and miquelianin were also determined as anti-inflammatory agents with 0.57 +/- 0.01mg/mL and 1.23 +/- 0.02mg/mL IC50 values, respectively. Total phenolic content and tannin content of the A.mollis and A.persica were determined as 357.00 +/- 75.80mg, 282.50 +/- 28.70mg PGE/g plant material and 18.02%, 18.63% respectively according to the method described by European Pharmacopoeia. Ellagic acid, miquelianin and catechin were analyzed by HPLC. The highest catechin content was detected in A.persica roots (6.69 +/- 0.05g/100g plant material). A.mollis aerial parts contain higher miquelianin (0.39 +/- 0.02g/plant material) and ellagic acid (1.56 +/- 0.01g/plant material) than A.persica.
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    In vivo activity assessment of some Tanacetum species used as traditional wound healer along with identification of the phytochemical profile by a new validated HPLC method
    (Mashhad Univ Med Sciences, 2018) Ozbilgin, Serkan; Akkol, Esra Kupeli; Oz, Burcin Ergene; Ilhan, Mert; Saltan, Gulcin; Acikara, Ozlem Bahadir; Tekin, Mehmet
    Objective(s): Tanacetum species are traditionally used as insecticide, and externally wound healer as well as for anti-inflammatory and antihistaminic properties. The in vivo wound-healing and anti-inflammatory potential of four Tanacetum species, Tanacetum argenteum (Lam.) Willd. subsp. argenteum (TA), Tanacetum heterotomum (Bornm.) Grierson (TH), Tanacetum densum (Lab.) Schultz Bip. subsp. sivasicum (TD), and Tanacetum vulgare L. (TV) was investigated. Materials and Methods: The chloroform (CHCl3) and methanol: water (80: 20) extracts were prepared from the aerial parts of each plant. For assessment of the wound-healing activity, linear incision on rats and circular excision on mice wound models were used and histopathological analyses were conducted on the tissues treated with the test materials. For the evaluation of the anti-inflammatory activity, Whittle Method based on the inhibition of the acetic acid-induced increase in capillary permeability was used. In order to elucidate the phytochemical contents of the extracts, HPLC profiles of active fractions were screened and quantitative analysis was conducted within the scope of HPLC analysis. Results: The CHCl3 extracts of TD, TA and TV were found to have significant wound healing activity (37.1%, 30.8% and 26.1% tensile strength; 88.05%, 72.93% and 44.88% contraction values, respectively) and anti-inflammatory activities (31.5% and 26.6% inhibition values for TD and TA). Parthenolide content of the CHCl3 extracts of TA, TH and TV were found 242.66 +/- 1.53, 190.16 +/- 5.62 and 177.51 +/- 3.73 mu g/100 mg plant material, respectively. Conclusion: According to the results, the other secondary metabolites present in the aerial parts of the Tanacetum species possibly exerted synergistic effects on the observed healing of the wounds.
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    Isolation and identification of f lavonol glycosides from Lathyrus armenus (Boiss. & Huet)
    (Briefland, 2020) Heydari, Hajar; Acikara, Ozlem Bahadir; Tekin, Mehmet; Iscan, Gulcin Saltan
    In the last five decades study on plant secondary metabolites have been increasing. Higher plants with a wide range of secondary metabolites have been very important in the search of new therapeutic agents. In this study secondary metabolites of Lathyrus armenus (Boiss. & Huet) which are endemic in Turkey, were studied. Flavonol glycosides (Rhamnocitrin-3-O-rhamninoside, Rhamnetin-3-O-rhamninoside, Rhamnazin-3-O-rhamninoside, kaempferol3-O-rhamninoside and, kaempferol-3-O-glucosyl (1 -> 2) rhamnoside) were isolated by different chromatographic methods and identified by H-1, C-13 NMR, as well as 2D NMR and Mass spectroscopy techniques from ethyl acetate and aqueous fractions of L. armenus's methanolic extract. This is the first study about secondary metabolites of Turkish Lathyrus species.