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    Development of callus colonies from the isolated microspore culture of capsicum annuum l.
    (2003) Bal U.; Abak K.; Büyükalaca S.; Comlekcioglu N.
    Androgenesis in Capsicum annuum L. via isolated microspore culture, with a limited scope, was studied. Donor genotype, i.e. U-130, was a doubled haploid. Intact flower buds containing microspores from early to late uninucleate stages were pretreated with a mannitol starvation medium both at 10 and 35°C for 7 days. High temperature treatment tested here, although considered effective in Capsicum anther culture, was detrimental and therefore flower buds from this treatment were not used. Following pretreatment at 10°C microspores were isolated and cultured on NLN medium (20) without growth regulators. Osmoticum and carbon was supplied to the microspores with 100, 130, and 170 g/1 sucrose. Only 170 g/1 culture was maintained and the rest of the cultures were lost to contamination. Callus colonies were obtained on the 30th and 60th days at rate of 40.5 and 67%, respectively. Callus colonies were subcultured to MS media containing 2, 4-D at 1 and 2 mg/1, but further development was not obtained. It was concluded that the approach adopted earlier on the cellular and molecular characterization studies of induced multinucleate Capsicum microspores (12, 14, 15) may lead to high frequency regeneration of haploids via callus colonies, as obtained in the present study, hence, isolated microspore culture using mannitol starvation as pretreatment has potentiality in Capsicum annum L. breeding. © 2003 Taylor and Francis Group, LLC.
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    Induction of symmetrical nucleus division and multicellular structures from the isolated microspores of lycopersicon esculentum mill
    (2005) Bal U.; Abak K.
    Effects of lactalbumin hydolysate (LH), casein hydrolysate (CH) and a starvation pretreatment using mannitol in the induction of symmetrical nucleus division of uninucleate microspores and formation of multicellular structures from the isolated microspores of tomato were studied. Flower buds containing uninucleate microspores were incubated on media for the pretreatment containing either mannitol or sucrose at 10 °C for seven days. Subsequently microspores were isolated and cultured on NLN basic media in the presence of BAP, NAA, LH and CH. Microspores from the cv. Fantastic, cultured in the presence of BAP at 0.5 mg l?1 and NAA at 0.5, 1.0 and 1.5 mg l?1 together with LH at 2 or 4 ml l?1 proceeded in the sporophytical pathway, microspore nuclei dividing symmetrically. Formation of multicellular structures were induced which was also the case for other cultivars cultured in the NLN medium containing 0.5 mg l?1 BAP and 0.5 mg l?1 NAA together with CH either at 5 or 10 g l?1. Mannitol pretreatment induced microspore starvation and stress therefore microspores from the mannitol pretreatment were more responsive to the symmetrical division than those from the sucrose pretreatment. © 2005 Taylor and Francis Group, LLC.