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Öğe Comparison of two different enzyme immunoassays in the diagnosis of fasciola hepatica infections(2004) Şakru N.; Korkmaz M.; Kuman H.A.In this study, the serum samples of 37 patients who were diagnosed as fascioliasis (=fasciolosis) by parasitologic, pathologic and/or radiographic methods, 51 patients who were previously infected with parasites other than Fasciola hepatica, and 31 healthy subjects, have been investigated for the presence of F.hepatica specific lgG antibodies with two different enzyme immunoassay (ELISA) methods. For this purpose, in-house DOT-ELISA and excretory-secretory (ES)-ELISA techniques have been performed by using F.hepatica ES antigens. All of the 37 fasciolosis patients found to be seropositive with ES-ELISA, while 36 were found positive with DOT-ELISA method. ES-ELISA method yielded 3 (5.9%) positive results for the patients with other parasitosis (2 toxocariasis, 1 hydatic cyst), and DOT-ELISA method gave positive results for 2 (3.9%) toxocariasis patients. In 31 healthy subjects, the seropositivity rates were detected as 3.2% and 16.1% for ES-ELISA and DOT-ELISA methods, respectively. As a result, the sensitivity and specificity of DOT-ELISA method were found as 97.3% and 93.3%, respectively, while these rates were 100% and 97.0% for ES-ELISA, respectively. The highest positive predictive value was detected for ES-ELISA (90.2%), while both of the methods showed high negative predictive values (100% for ES-ELISA, 99.1% for DOT-ELISA). In conclusion, ELISA methods would be useful especially for the laboratory diagnosis of fasciolosis, in the clinically suspected patients where the etiologic agent could not be identified.Öğe Genotyping of Giardia intestinalis isolates in the Thrace region, Turkey(Ankara Microbiology Society, 2015) Çiçek C.; Şakru N.Giardia intestinalis is a common protozoon that infects humans and may cause water and food-borne outbreaks. It is regarded as a major public health problem worldwide and in Turkey as well. Molecular techniques are widely used to determine the epidemiology, genetic populations and taxonomy of G.intestinalis. It has two genotypes including genotype A and genotype B in humans. The purpose of the present study is to implement the molecular analysis and genotyping of the isolates of G.intestinalis obtained from human stool samples. A total of 39 isolates obtained from the stool samples of persons (30 male, 9 female; age range: 1-74 years, median age: 20) who have admitted to Trakya University Medical Research and Practice Health Center and Edirne State Hospital between September 2011- April 2013 were included in the study. The average number of cysts were identified both with native and lugol methods among all microscopically detected samples by screening at least 50 field with x4OO magnification. The samples were then analyzed through loop-mediated isothermal amplification method (LAMP) for the presence of elongation factor-1 alpha (EF-l?) gene, and polymerase chain reaction (PCR) method for the presence of beta-giardin (bg) gene regions. In addition, sequence analysis of bg gene was performed. Of 39 samples, 32 (82%) and 19 (48.7%) were found to be positive for G.intestinalis EF-1? and bg genes by LAMP and PCR methods, respectively. Genotyping was implemented in 17 out of 19 samples yielding nine genotype A and eight genotype B strains. The sub-genotypes of these strains were identified as A2 (n= 6), A3 (n= 3), B2 (n= 6), B3 (n= 1) and B4 (n= 1). In eight isolates that could be typed among 21 symptomatic patients, genotype B (n= 5) and in nine isolates that could be typed among 18 asymptomatic patients, genotype A (n= 6) were more frequently observed. There was no significant association between symptomatic or asymptomatic status and genotypic patterns of the cases (p= 0.347). The PCR positivity rate showed a significant difference between patients with higher cyst density and lower cyst density (p= 0.0001). In conclusion, molecular methods such as LAMP and PCR might have the potential to provide a substantial guidance for the analysis of outbreaks. In addition, the determined subtypes of C.intestinalis in our region is expected to contribute to the global epidemiological data.Öğe Retrospective Evaluation of Toxoplasma gondii Serological Test Results of Trakya University Health Center for Medical Research and Practice(Galenos Publishing House, 2023) Eryıldız C.; Çakmakçı B.K.; Aldı F.İ.; Şakru N.Objective: Toxoplasma gondii (T. gondii) is an obligate intracellular parasite distributed worldwide. Serological tests investigating antibodies specific to T. gondii are widely used in diagnosis. The aim of this study was to evaluate the results of anti-T. gondii IgG, anti-T. gondii IgM, and anti-T. gondii IgG avidity tests, which were sent to the Serology Laboratory of Trakya University Health Center for Medical Research and Practice, retrospectively. Methods: Anti-T. gondii IgM, anti-T. gondii IgG, and anti-T. gondii IgG avidity tests were studied by enzyme-linked fluorescent assay or electrochemiluminescence immunoassay method between January 2012 and December 2021. The test results were evaluated retrospectively from laboratory records. Results: Of 18,659 serum samples were studied for anti-T. gondii IgG, 5,127 (27.5%) samples were positive, whereas 721 (3.4%) of 21,108 samples were positive for anti-T. gondii IgM. Of the 593 serum samples tested for IgG avidity, 206 (34.7%) samples had low avidity, 118 (19.9%) had borderline, and 269 (45.4%) had high avidity. Conclusion: Our study, compatible with other studies, showed that seropositivity is high in our region, which is not negligible. Especially in women of reproductive age population, T. gondii should be considered in suspected clinical cases. © 2023 Turkish Society for Parasitology.Öğe Serological monitoring of paediatric visceral leishmaniasis By IFA and ELISA methods.(2011) Şakru N.; Töz S.O.; Korkmaz M.; Özbel Y.Visceral leishmaniasis (VL) is endemic in all Mediterranean countries including Turkey, and children are at greater risk than adults in endemic areas. In VL patients, serological assays are considered to be sensitive for the diagnosis and/or follow up. The aim of this study was to assess the usefulness of serology for following up of childhood VL in Turkey. Sera obtained from twenty parasitologically confirmed children with VL were tested using IFAT and ELISA. The patients were monitored clinically and serologically (range: 20-500 days) during and after treatment. All VL patients were treated with meglumine antimonate. Anti-Leishmania antibodies in successfully treated VL patients showed a steep decline but, in three patients who had relapsed, an increase was detected. Significantly lower values were observed after treatment with both serological techniques. Mean ELISA optical density values before and after treatment were: 0.78 ± 0.36 (0.26-1.76) and 0.38 ± 0.24 (0.09-0.83) respectively, (p < 0.001) and mean IFAT values (log10 transformed titers) before and after treatment were: 3.02 ± 0.90 (1.81-4.51) and 2.16 ± 0.75 (1.20-3.90) respectively, (p < 0.001). ELISA and IFAT are valuable not only for diagnosis but also for monitoring of drug therapy in childhood visceral leishmaniasis as rapid and non-invasive techniques.Öğe Seroprevalence of Leishmaniasis Among Dogs Living in a Municipal Dog and Cat Shelter in Edirne(2016) Düzbeyaz A.; Şakru N.; Töz S.OBJECTIVE: In this study, we aimed to investigate the seroprevalence of canine leishmaniosis among dogs that live in the town center due to a lack of data on the prevalence of canine leishmaniasis (CanL) in Edirne.METHODS: In the present study, 37 dogs living in a municipal dog and cat shelter in Edirne were screened for leishmaniosis by the ındirect fluorescent antibody test.RESULTS: All samples were found to be seronegative.CONCLUSION: Our study is a preliminary study for Edirne. We wish to perform a large-scale seroepidemiological study with a larger number of dogs from different regions and identify Phlebotomus species.Öğe A Small Bite on the Eyelid(NLM (Medline), 2019) Şakru N.; Taylan Özkan A.; Benian Ö.; Aksoy M.D.[No abstract available]
